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Home / Equine Research Bank / Int J Mol Sci / In Vitro Immunomodulatory Effects of Equine Adipose Tissue-D...
International journal of molecular sciences2025; 26(9); doi: 10.3390/ijms26094208

In Vitro Immunomodulatory Effects of Equine Adipose Tissue-Derived Mesenchymal Stem Cells Primed with a Cannabidiol-Rich Extract.

Abstract: Cell-based therapy using mesenchymal stem cells (MSCs) shows promise for treating several diseases due to their anti-inflammatory and immunomodulatory properties. To enhance the therapeutic potential of MSCs, in vitro priming strategies have been explored. Cannabidiol (CBD), a non-psychoactive compound derived from cannabis, may influence MSC proliferation, differentiation, and immunomodulatory properties. This study evaluates the immunomodulatory potential of equine adipose tissue-derived MSCs (EqAT-MSCs) primed with a CBD-rich cannabis extract. EqAT-MSCs (P3) were primed with CBD concentrations of 5 µM and 7 µM for 24 h. Morphological analysis, MTT assay, β-galactosidase activity, apoptosis assays, and gene expression of interleukins IL-1β, IL-6, IL-10, interferon-gamma (IFN-γ), and tumor necrosis factor-alpha (TNF-α) were conducted. Additionally, cannabinoid receptor 1 (CB1) and 2 (CB2) expression were evaluated in naïve EqAT-MSCs (P2-P5). The naïve EqAT-MSCs expressed CB1 and CB2 receptors. Priming with 5 µM significantly increased the expression of IL-10, TNF-α, and IFN-γ, while 7 µM decreased IL-1β and IL-6 expression. No significant changes were observed in other cytokines, MTT, β-galactosidase activity, or apoptosis. These findings demonstrate that naïve EqAT-MSCs express CB1 and CB2 receptors and priming with the extract modulates the expression of pro- and anti-inflammatory cytokines, highlighting its potential immunomodulatory role in EqAT-MSC-based therapies.
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Publication Date: 2025-04-29 PubMed ID: 40362445PubMed Central: PMC12071624DOI: 10.3390/ijms26094208Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates how priming horse fat-derived stem cells with Cannabidiol (CBD), a non-intoxicating compound from cannabis, may enhance their therapeutic potential due to modifying their proliferation, differentiation, and immune regulating properties.

Objective of the Research

  • The main objective of this research was to study the immunomodulatory potential of equine adipose tissue-derived mesenchymal stem cells (MSCs) that had been treated with a CBD-rich extract. This was to understand if CBD has the potential to enhance the therapeutic application of these MSCs by changing their properties like proliferation and differentiation.

Research Methodology

  • The equine MSCs were treated with CBD concentrations of 5 µM and 7 µM for 24 hours and subsequently subjected to multiple tests including morphological analysis, MTT assay, β-galactosidase activity, apoptosis assays, and gene expression analysis.
  • The gene expression of various interleukins (IL-1β, IL-6, IL-10) and other factors like interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) were examined.
  • The research also looked at the expression of cannabinoid receptors 1 (CB1) and 2 (CB2) in untreated (naïve) equine MSCs.

Research Findings

  • The study found that untreated equine MSCs already expressed CB1 and CB2 receptors.
  • Treating the cells with 5 µM of CBD significantly increased the expression of IL-10, TNF-α, and IFN-γ. On the other hand, a 7 µM treatment decreased IL-1β and IL-6 expression.
  • No significant changes were observed in other cytokines, in the MTT, β-galactosidase activity, or apoptosis.

Implication of the Findings

  • The research shows that treating equine MSCs with CBD-rich extract modulates the expression of certain pro- and anti-inflammatory cytokines, indicating that CBD could potentially have an immunomodulatory role in MSC-based therapy.
  • This could enhance the therapeutic potential of stem cell therapy in treating various diseases that involve inflammation and immune system activity.

Cite This Article

APA
Battistin L, Moya LFA, Ferreira LVO, Braz AMM, Carvalho M, Golim MA, Amorim RM. (2025). In Vitro Immunomodulatory Effects of Equine Adipose Tissue-Derived Mesenchymal Stem Cells Primed with a Cannabidiol-Rich Extract. Int J Mol Sci, 26(9). https://doi.org/10.3390/ijms26094208

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 26
Issue: 9

Researcher Affiliations

Battistin, Lorena
  • Department of Veterinary Clinic, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618-681, SP, Brazil.
Moya, Luís Felipe Arantes
  • Department of Veterinary Clinic, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618-681, SP, Brazil.
Ferreira, Lucas Vinícius de Oliveira
  • Department of Veterinary Clinic, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618-681, SP, Brazil.
  • Center for Translational Research in Regenerative Medicine, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618-681, SP, Brazil.
Braz, Aline Márcia Marques
  • Laboratory of Applied Biotechnology, Clinical Hospital of the Medical School, São Paulo State University (UNESP), Botucatu 18618-687, SP, Brazil.
Carvalho, Márcio de
  • Department of Veterinary Clinic, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618-681, SP, Brazil.
Golim, Marjorie de Assis
  • Laboratory of Applied Biotechnology, Clinical Hospital of the Medical School, São Paulo State University (UNESP), Botucatu 18618-687, SP, Brazil.
Amorim, Rogério Martins
  • Department of Veterinary Clinic, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618-681, SP, Brazil.
  • Center for Translational Research in Regenerative Medicine, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu 18618-681, SP, Brazil.

MeSH Terms

  • Animals
  • Cannabidiol / pharmacology
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / immunology
  • Mesenchymal Stem Cells / metabolism
  • Horses
  • Adipose Tissue / cytology
  • Plant Extracts / pharmacology
  • Immunomodulation / drug effects
  • Apoptosis / drug effects
  • Cytokines / metabolism
  • Cell Differentiation / drug effects
  • Cells, Cultured
  • Receptor, Cannabinoid, CB2 / metabolism
  • Cell Proliferation / drug effects

Grant Funding

  • This study was funded by the São Paulo Research Foundation (FAPESP) (2022/08919-2 and 2022/16418-3). Additionally, the study was supported by a doctoral scholarship granted by FAPESP (2024/19980-0). / FAPESP

Conflict of Interest Statement

The authors declare no conflicts of interest.

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