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Home / Equine Research Bank / Z Parasitenkd / In vitro induction of lymphocyte responsiveness by a Strongy...
Zeitschrift fur Parasitenkunde (Berlin, Germany)1984; 70(2); 229-242; doi: 10.1007/BF00942226

In vitro induction of lymphocyte responsiveness by a Strongylus vulgaris-derived mitogen.

Abstract: Proliferation in vitro of peripheral blood lymphocytes both from horses infected with Strongylus vulgaris and from helminth-free ponies was observed in the presence of extracts of the fourth and fifth stage larvae and adults of S. vulgaris. In addition, S. vulgaris extracts induced transformation in cultures of peripheral blood lymphocytes from sheep and dogs and in mouse spleen cell cultures. Nylon wool non-adherent, T cell enriched fractions of lymphocytes from both mice and horses were stimulated by the S. vulgaris larval mitogen while no proliferation was observed in cultures containing nylon wool adherent, B cell enriched fractions. Macrophage co-operation appeared not to be necessary for S. vulgaris mitogen-induced transformation of spleen cells. The S. vulgaris mitogen stimulated a subpopulation of mouse spleen cells different from those responsive to PHA, Con A and LPS. These cells might be T helper cells since B cells were stimulated to proliferate in the presence of both T cells and S. vulgaris larval mitogen. In addition, the supernatant of in vitro cultured larvae of S. vulgaris induced slight, but significant transformation of equine peripheral blood lymphocytes. Therefore, it is possible that the S. vulgaris mitogen released by both viable parasites and degenerating larvae might induce T cell dependent production of immunoglobulin in vivo and account for the beta-globulinaemia, of which IgG(T) is a major component, in S vulgaris infected horses.
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Publication Date: 1984-01-01 PubMed ID: 6609495DOI: 10.1007/BF00942226Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper discusses the observation that the larval and adult extracts of the parasite Strongylus vulgaris can stimulate the proliferation of different types of lymphocytes from various animals in a laboratory setting, which may shed light on the body’s immune response to this parasite and the resulting infection.

Objective of the Research

  • This research sought to understand the behavior of particular immune cells, lymphocytes, in the presence of antigens derived from different stages of Strongylus vulgaris, a parasite that affects horses.

Research Findings

  • The authors found that lymphocytes not only from horses infected with S. vulgaris but also from helminth-free ponies, proliferated in the presence of extracts from the larvae and adult S. vulgaris.
  • Moreover, the study also found that the S. vulgaris extracts induced the transformation of other animals’ lymphocytes, specifically from sheep, dogs, and mice.
  • The study identified that the mitogen, an agent that encourages the division and proliferation of cells, from S. vulgaris stimulated non-adherent (floating), T-cell enriched fractions of lymphocytes in mice and horses.
  • In contrast, the mitogen did not stimulate proliferation in those lymphocytes that clung to the nylon wool, a.k.a, B cells, another type of lymphocyte.
  • The researchers found that the interaction of the S. vulgaris mitogen did not seem to require any macrophage cooperation, macrophages are cells that eat cellular debris and pathogens.
  • The S. vulgaris mitogen affected a subpopulation of spleen cells in mice differently than other known mitogens, including PHA, Con A, and LPS. This suggests that the mitogen targets a specific group of immune cells that might be T helper cells since B cells began to proliferate when T cells were present along with the S. vulgaris mitogen.
  • Finally, this research also discovered that the supernatant, the fluid containing the dissolved substances, of the in vitro cultured larvae of S. vulgaris caused a slight but significant transformation of equine peripheral blood lymphocytes.

Implications of the Research

  • The authors proposed that the S. vulgaris mitogen released by both living and degenerating parasites might stimulate in vivo T-cell dependent production of immunoglobulin, an immune protein that the body produces in response to an antigen, which could explain the overproduction of beta-globulin (where IgG(T) is a major component) in horses infected with S. vulgaris.

Cite This Article

APA
Bailey M, Lloyd S, Martin SC, Soulsby EJ. (1984). In vitro induction of lymphocyte responsiveness by a Strongylus vulgaris-derived mitogen. Z Parasitenkd, 70(2), 229-242. https://doi.org/10.1007/BF00942226

Publication

Zeitschrift fur Parasitenkunde (Berlin, Germany)
ISSN: 0044-3255
NlmUniqueID: 8710749
Country: Germany
Language: English
Volume: 70
Issue: 2
Pages: 229-242

Researcher Affiliations

Bailey, M
    Lloyd, S
      Martin, S C
        Soulsby, E J

          MeSH Terms

          • Animals
          • Antigens / immunology
          • B-Lymphocytes / immunology
          • Dogs / immunology
          • Horses / immunology
          • Larva / immunology
          • Lectins / pharmacology
          • Lymphocyte Activation
          • Lymphocytes / immunology
          • Mice / immunology
          • Sheep / immunology
          • Spleen / cytology
          • Strongyle Infections, Equine / immunology
          • Strongyloidea / immunology
          • T-Lymphocytes / immunology

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          Citations

          This article has been cited 3 times.
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