In vitro maturation and artificial activation of donkey oocytes.
Abstract: Three media were evaluated for their ability to support in vitro maturation of donkey (Equus asinus) oocytes and their development after parthenogenetic activation. The basal medium for Medium 1 (M1) and Medium 2 (M2) was M199 and DMEM/F12 respectively, whereas, Medium 3 (M3) consisted of equal parts (v/v) of M199 and DMEM/F12. All three media were supplemented with 10% (v/v) fetal calf serum, 0.01 units/mL porcine FSH, 0.01 units/mL equine LH, 200 ng/mL insulin-like growth factor 1(IGF-I), 10 μl/mL insulin-transferrin-selenium (ITS), 0.1 mg/mL taurine, 0.1 mg/mL L-cysteine, 0.05 mg/mL L-glutamine, 0.11 mg/mL sodium pyruvate, and 25 mg/mL gentamycin. There were no significant differences among the three maturation media for oocyte maturation. Maturation rate of donkey oocytes in M1 was 53% for compact (Cp) cumulus-oocyte complexes and 75% for expanded (Ex) cumulus-oocyte complexes; in M2 these were 55 and 77%, respectively; and in M3, 58 and 75%. The percentage of cleaved parthenotes and 4- or 8-cell embryos were not significantly different for oocytes matured in the various media (61 and 24% for M1; 66 and 32% for M2; and 67 and 33% for M3). Oocytes matured in M3 tended to yield a higher rate of advanced embryo development (morula) than oocytes matured in M1 (22 vs 9%; P = 0.07). In conclusion, donkey oocytes were matured and parthenogenetically activated in vitro, using methods similar to those used in the horse.
Copyright © 2011 Elsevier Inc. All rights reserved.
Publication Date: 2011-05-23 PubMed ID: 21601263DOI: 10.1016/j.theriogenology.2010.10.039Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research is about the in vitro maturation and artificial activation of donkey oocytes using three different media supplements. The results showed no significant differences among the three media for oocyte maturation and parthenogenetic activation, although Medium 3 showed a tendency for higher embryo development.
Study Design and Methodology
- The research evaluated three media for their ability to support in vitro maturation of donkey oocytes and their development after parthenogenetic activation.
- The basal medium for Medium 1 (M1) and Medium 2 (M2) was M199 and DMEM/F12 respectively, while Medium 3 (M3) was a combination of half parts of M199 and DMEM/F12.
- All three media were complemented with several supplements including fetal calf serum, porcine FSH, equine LH, insulin-like growth factor 1(IGF-I), insulin-transferrin-selenium (ITS), taurine, L-cysteine, L-glutamine, sodium pyruvate, and gentamycin. These substances were included to provide a favorable environment for the oocytes to mature.
Findings
- The study found no significant differences in the maturation rates of donkey oocytes for the three media. The maturation rate in M1 was 53% for compact cumulus-oocyte complexes and 75% for expanded ones; in M2, 55% and 77%, respectively; and in M3, 58% and 75%.
- The percentage of cleaved parthenotes (an early stage in the development of an organism produced by parthenogenesis) and 4- or 8-cell embryos were also not significantly different. The respective rates in M1 were 61% and 24%, in M2 it was 66% and 32%, and in M3 it was 67% and 33%.
Conclusion
- The study concluded that donkey oocytes could be matured and activated in vitro using methods like those used in horses. It also indicated that oocytes matured in M3 tended to have higher rates of advanced embryo development compared to those matured in M1, though this difference was not statistically significant.
Cite This Article
APA
Zhao G, Wu K, Cui L, Zhao L, Liu Y, Tan X, Zhou H.
(2011).
In vitro maturation and artificial activation of donkey oocytes.
Theriogenology, 76(4), 700-704.
https://doi.org/10.1016/j.theriogenology.2010.10.039 Publication
Researcher Affiliations
- College of Animal Science and Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China.
MeSH Terms
- Animals
- Cell Culture Techniques
- Chi-Square Distribution
- Culture Media
- Equidae / physiology
- Female
- Oocytes / cytology
- Oocytes / physiology
- Parthenogenesis / physiology
Citations
This article has been cited 3 times.- Abdel-Salam Z, Harith MA. Laser researches on livestock semen and oocytes: A brief review. J Adv Res 2015 May;6(3):311-7.
- Abdoon AS, Abdel-Rahman HA, Shawki SM, Kandil OM, Fathalla SI. Influence of follicle size, methods of retrieval on oocytes yield and morphology in Egyptian Jennies ovaries with special reference to maturation rate in vitro. Vet Res Commun 2014 Dec;38(4):287-95.
- Gambini A, Smith JM, Gurkin RJ, Palacios PD. Current and Emerging Advanced Techniques for Breeding Donkeys and Mules. Animals (Basel) 2025 Mar 29;15(7).
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