In vitro model for testing novel implants for equine laryngoplasty.

This study focuses on developing a laboratory-based model for testing different laryngoplasty techniques on horses. The researchers utilised cadaveric equine larynges with laryngoplasty sutures to mimic airflow and pressure conditions experienced by horses during maximum exercise.

Methods Used in the Research

• The study used a randomized complete block method.
• Postmortem equine larynges (n=10) were utilized for the research.
• Prosthetic laryngoplasty sutures were placed on the larynges bilaterally to create maximal arytenoid abduction.
• Each larynx was seated in a flow chamber with both static and dynamic flow conditions at a frequency of 2 Hz.
• The tracheal pressure and flow, as well as the pressure within the flow chamber, were measured at a sampling frequency of 500 Hz.
• The obtained data set was compared with well-established physiological values relative to horses doing maximal exercise.

Results of the Study

• The study showed that under static flow conditions, the average inspiratory tracheal pressure was -33.0+/-0.98 mm Hg, with a flow rate of 54.48+/-1.8 L/s.
• The pressure within the flow chamber reached -8.1+/-2.2 mm Hg, thus producing a translaryngeal impedance of 0.56+/-0.15 mm Hg/L/s.
• Under dynamic conditions, the model created inspiratory pharyngeal and tracheal pressures of -8.85+/-2.5 and -35.54+/-1.6 mm Hg, respectively.
• The peak inspiratory flow achieved was 51.65+/-2.3 L/s, leading to an impedance of 0.57+/-0.06 mm Hg/L/s.

Conclusion of the Study

• The laboratory model generated inspiratory pressures that closely resemble those experienced by horses during maximal exercise.
• This model proves useful in testing several new techniques for laryngoplasty, potentially leading to the evolution of improved techniques.