In-vitro modulation of plasminogen activator activity, prostaglandin E and nitric oxide production by interleukin-1 in pregnant mare serum gonadotrophin-primed theca-interstitial cells.
Abstract: To examine the participation of the theca-interstitial (TI) compartment in cytokine modulation of ovarian function, the effects of interleukin-1beta (IL-1) on plasminogen activator (PA) activity and on prostaglandin E (PGE) and nitric oxide (NO) production were examined in cultures of pregnant mare serum gonadotrophin (PMSG)-primed rat TI cells. Exposure to IL-1 (10 ng/ml) resulted in a 25% reduction (P < 0.001) in PA activity, concurrent with a 4.6-fold increase in the ability of the corresponding conditioned media to inhibit exogenous urokinase activity. IL-1 also produced a 4.7-fold increase in PGE content and a 2.8-fold increase in NO generation. These effects of IL-1 were abolished by the IL-1 receptor antagonist, suggesting specific IL-1 receptor-mediated effects. Transforming growth factor (TGF)-beta1 (10 ng/ml) significantly attenuated the IL-1-stimulated PGE production and NO generation but did not affect the ability of IL-1 to suppress PA activity and stimulate urokinase inhibitor production. The NO synthase inhibitor N-nitro-L-arginine attenuated the IL-1-induced NO generation but had no effect on PA activity or PGE production. Thus, NO is not an obligatory mediator of IL-1 effects on plasminogen activation and PGE generation in rat ovary. The present observations attest to a pleiotropic response of PMSG-primed TI cells to IL-1, and suggest a paracrine/autocrine function for the TI compartment in ovulation and corpus luteum formation.
Publication Date: 1997-04-01 PubMed ID: 9159441DOI: 10.1093/humrep/12.4.774Google Scholar: Lookup
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- Journal Article
Summary
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The research investigates how the cytokine, interleukin-1beta (IL-1), influences the function of theca-interstitial cells in rat ovaries pre-treated with pregnant mare serum gonadotrophin (PMSG), particularly looking at its effects on plasminogen activator activity, prostaglandin E generation, and nitric oxide production.
Objective and Methodology
- The objective of this research was to examine the role of the theca-interstitial (TI) compartment in the ovarian function, especially how it’s modulated by longer lashes-1beta (IL-1).
- This was accomplished by observing the effects of IL-1 on plasminogen activator (PA) activity and on the production of prostaglandin E (PGE) and nitric oxide (NO) in PMSG-primed rat TI cells.
Results and Observations
- The research showed that exposure to IL-1 resulted in a 25% decrease in PA activity, this was along with an increase in the ability of the conditioned media to inhibit urokinase activity — a substance involved in breaking down blood clots.
- IL-1 also led to a significant increase in PGE content and NO generation.
- These effects were found to be linked to IL-1 receptor activation as they could be inhibited by the IL-1 receptor antagonist.
Additional Investigations
- The study also found that the results could be moderated by transforming growth factor (TGF)-beta1, which significantly reduced the IL-1 stimulated PGE production and NO generation, but did not affect the ability of IL-1 to suppress PA activity and stimulate urokinase inhibitor production.
- The effects of a NO synthase inhibitor were also tested. This attenuated IL-1-induced NO generation but had no effect on PA activity or PGE production, indicating that NO is not a key mediator in IL-1 impacts on plasminogen activation and PGE generation in rat ovaries.
Conclusions
- The study concluded that IL-1 has broad and varied effects on PMSG-primed TI cells, hinting at a paracrine/autocrine function for the TI compartment in ovulation and corpus luteum formation processes.
This study has significant implications for understanding cytokine modulation of ovarian function, particularly within the context of ovulation and corpus luteum formation processes.
Cite This Article
APA
Hurwitz A, Finci-Yeheskel Z, Milwidsky A, Yagel S, Adashi EY, Laufer N, Mayer M.
(1997).
In-vitro modulation of plasminogen activator activity, prostaglandin E and nitric oxide production by interleukin-1 in pregnant mare serum gonadotrophin-primed theca-interstitial cells.
Hum Reprod, 12(4), 774-779.
https://doi.org/10.1093/humrep/12.4.774 Publication
Researcher Affiliations
- Department of Obstetrics/Gynecology, Hadassah University Hospital, Mount Scopus, Jerusalem, Israel.
MeSH Terms
- Animals
- Cells, Cultured
- Female
- Gonadotropins, Equine / pharmacology
- Interleukin-1 / physiology
- Nitric Oxide / biosynthesis
- Plasminogen Activator Inhibitor 1 / metabolism
- Plasminogen Activators / metabolism
- Prostaglandins E / biosynthesis
- Rats
- Rats, Inbred Strains
- Theca Cells / drug effects
- Transforming Growth Factor beta / pharmacology
- Urokinase-Type Plasminogen Activator / antagonists & inhibitors
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