In vitro susceptibility of equine-obtained isolates of Corynebacterium pseudotuberculosis to gallium maltolate and 20 other antimicrobial agents.
Abstract: This study's objective was to determine the in vitro antimicrobial activities of gallium maltolate (GaM) and 20 other antimicrobial agents against clinical equine isolates of Corynebacterium pseudotuberculosis. The growth of cultured isolates was not inhibited by any concentration of GaM. MIC data revealed susceptibility to commonly used antimicrobials.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Publication Date: 2014-05-14 PubMed ID: 24829243PubMed Central: PMC4097677DOI: 10.1128/JCM.01252-14Google Scholar: Lookup
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- Journal Article
- Research Support
- N.I.H.
- Extramural
- Research Support
- Non-U.S. Gov't
Summary
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The research focuses on testing the ability of gallium maltolate and 20 other antimicrobial substances to inhibit growth of Corynebacterium pseudotuberculosis, a pathogen that commonly affects horses. Evidently, gallium maltolate showed no effectiveness while the other antimicrobials proved to be potent.
Research Objective
- The main aim of the research was to explore and quantify the antimicrobial effectiveness of gallium maltolate, as well as that of 20 other antimicrobial agents, against clinical isolates of Corynebacterium pseudotuberculosis, a bacterial pathogen that has direct impact on the health of horses.
Methodology
- The researchers used an in vitro laboratory setup to mimic the conditions where the pathogen is usually found.
- These pathogens were cultured in a controlled environment and then exposed to various concentrations of gallium maltolate, and 20 other antimicrobial compounds.
Findings
- The findings revealed that gallium maltolate was unable to inhibit the growth of the bacteria at any concentration it was tried at. This implies that gallium maltolate may not be a suitable treatment option for controlling or eliminating Corynebacterium pseudotuberculosis infections in equines.
- However, the Minimum Inhibitory Concentration (MIC) data indicated that the bacterium showed susceptibility against other commonly used antimicrobials. This translates to the fact that the other 20 studied antimicrobials could indeed be effective at combating Corynebacterium pseudotuberculosis infections.
Importance of the Study
- The study is significant in the field of equine health as it explores potential treatment options for infections caused by the Corynebacterium pseudotuberculosis bacterium.
- It provides valuable insight into the effectiveness, or lack thereof, of gallium maltolate in this particular instance, contributing to the wider knowledge base surrounding the treatment of such infections.
- Moreover, the results about other antimicrobials can guide future research and practical applications for treating Corynebacterium pseudotuberculosis infections in horses.
Cite This Article
APA
Norman TE, Batista M, Lawhon SD, Zhang S, Kuskie KR, Swinford AK, Bernstein LR, Cohen ND.
(2014).
In vitro susceptibility of equine-obtained isolates of Corynebacterium pseudotuberculosis to gallium maltolate and 20 other antimicrobial agents.
J Clin Microbiol, 52(7), 2684-2685.
https://doi.org/10.1128/JCM.01252-14 Publication
Researcher Affiliations
- Equine Infectious Disease Laboratory, Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA tnorman@cvm.tamu.edu.
- Equine Infectious Disease Laboratory, Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
- Clinical Microbiology Laboratory, Department of Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
- Clinical Microbiology Laboratory, Department of Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
- Equine Infectious Disease Laboratory, Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
- Texas Veterinary Medical Diagnostic Laboratory, College Station, Texas, USA.
- Terrametrix, Menlo Park, California, USA.
- Equine Infectious Disease Laboratory, Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
MeSH Terms
- Animals
- Anti-Infective Agents / pharmacology
- Corynebacterium Infections / microbiology
- Corynebacterium Infections / veterinary
- Corynebacterium pseudotuberculosis / drug effects
- Corynebacterium pseudotuberculosis / isolation & purification
- Horse Diseases / microbiology
- Horses
- Microbial Sensitivity Tests
- Organometallic Compounds / pharmacology
- Pyrones / pharmacology
References
This article includes 12 references
- Gavan TL, Jones RN, Barry AL. Evaluation of the Sensititre system for quantitative antimicrobial drug susceptibility testing: a collaborative study.. Antimicrob Agents Chemother 1980 Mar;17(3):464-9.
- Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; approved standard, 3rd ed.. 2008.
- Dorella FA, Pacheco LG, Oliveira SC, Miyoshi A, Azevedo V. Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence.. Vet Res 2006 Mar-Apr;37(2):201-18.
- D'Afonseca V, Moraes PM, Dorella FA, Pacheco LG, Meyer R, Portela RW, Miyoshi A, Azevedo V. A description of genes of Corynebacterium pseudotuberculosis useful in diagnostics and vaccine applications.. Genet Mol Res 2008 Mar 18;7(1):252-60.
- Coleman M, Kuskie K, Liu M, Chaffin K, Libal M, Giguère S, Bernstein L, Cohen N. In vitro antimicrobial activity of gallium maltolate against virulent Rhodococcus equi.. Vet Microbiol 2010 Nov 20;146(1-2):175-8.
- Harrington JR, Martens RJ, Cohen ND, Bernstein LR. Antimicrobial activity of gallium against virulent Rhodococcus equiin vitro and in vivo.. J Vet Pharmacol Ther 2006 Apr;29(2):121-7.
- Stolle P, Barckhausen O, Oehlmann W, Knobbe N, Vogt C, Pierik AJ, Cox N, Schmidt PP, Reijerse EJ, Lubitz W, Auling G. Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y˙) involved in ribonucleotide reduction.. FEBS J 2010 Dec;277(23):4849-62.
- Soares SC, Silva A, Trost E, Blom J, Ramos R, Carneiro A, Ali A, Santos AR, Pinto AC, Diniz C, Barbosa EG, Dorella FA, Aburjaile F, Rocha FS, Nascimento KK, Guimarães LC, Almeida S, Hassan SS, Bakhtiar SM, Pereira UP, Abreu VA, Schneider MP, Miyoshi A, Tauch A, Azevedo V. The pan-genome of the animal pathogen Corynebacterium pseudotuberculosis reveals differences in genome plasticity between the biovar ovis and equi strains.. PLoS One 2013;8(1):e53818.
- Billington SJ, Esmay PA, Songer JG, Jost BH. Identification and role in virulence of putative iron acquisition genes from Corynebacterium pseudotuberculosis.. FEMS Microbiol Lett 2002 Feb 19;208(1):41-5.
- Costa LR, Spier SJ, Hirsh DC. Comparative molecular characterization of Corynebacterium pseudotuberculosis of different origin.. Vet Microbiol 1998 May;62(2):135-43.
- Foley JE, Spier SJ, Mihalyi J, Drazenovich N, Leutenegger CM. Molecular epidemiologic features of Corynebacterium pseudotuberculosis isolated from horses.. Am J Vet Res 2004 Dec;65(12):1734-7.
- Judson R, Songer JG. Corynebacterium pseudotuberculosis: in vitro susceptibility to 39 antimicrobial agents.. Vet Microbiol 1991 Apr;27(2):145-50.
Citations
This article has been cited 4 times.- Santos LM, Rodrigues DM, Kalil MA, Azevedo V, Meyer R, Umsza-Guez MA, Machado BA, Seyffert N, Portela RW. Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm.. Front Vet Sci 2021;8:700030.
- Kalil MA, Santos LM, Barral TD, Rodrigues DM, Pereira NP, Sá MDCA, Umsza-Guez MA, Machado BAS, Meyer R, Portela RW. Brazilian Green Propolis as a Therapeutic Agent for the Post-surgical Treatment of Caseous Lymphadenitis in Sheep.. Front Vet Sci 2019;6:399.
- Santos LM, Stanisic D, Menezes UJ, Mendonça MA, Barral TD, Seyffert N, Azevedo V, Durán N, Meyer R, Tasic L, Portela RW. Biogenic Silver Nanoparticles as a Post-surgical Treatment for Corynebacterium pseudotuberculosis Infection in Small Ruminants.. Front Microbiol 2019;10:824.
- Giacani L, Bernstein LR, Haynes AM, Godornes BC, Ciccarese G, Drago F, Parodi A, Valdevit S, Anselmi L, Tomasini CF, Baca AM. Topical treatment with gallium maltolate reduces Treponema pallidum subsp. pertenue burden in primary experimental lesions in a rabbit model of yaws.. PLoS Negl Trop Dis 2019 Jan;13(1):e0007076.
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