In vivo determination of surface tension in the horse trachea and in vitro model studies.
Abstract: We measured the surface tension in the trachea of the non-anaesthetised horse from the spreading behaviour of fluid drops, using videotracheoscopy. To do this, we placed small oil drops onto the tracheal wall with a thin Teflon tubing inserted into a videocolonoscope used in humans. Either 5 ml of saline (control) or 5 ml of bovine lipid extract surfactant (BLES) at 4 mg/ml were administered. Tracheal surface tension was 31.9 +/- 0.54 mN/m (Mean +/- SEM, n = 30) in the control experiments and 24.5 +/- 0.51 mN/m (Mean +/- SEM, n = 21) in the entire trachea after the administration of BLES. These values were determined from calibration curves relating film surface tension to the relative diameter of test fluid droplets. In the calibration experiments, the test fluid droplets were placed onto a surfactant film at various surface tensions in either a modified Langmuir-Wilhelmy balance or a captive bubble surfactometer. The spreading behaviour of a given test fluid droplet in the model studies did not only depend on the film surface tension but also on the thickness of the aqueous layer below the surfactant film. Hence, the computed surface tensions in the trachea depend on the choice of which in vitro model is applied.
Publication Date: 1997-07-01 PubMed ID: 9271810DOI: 10.1016/s0034-5687(97)84032-7Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article discusses a method to measure surface tension in the trachea of a non-anesthetized horse using videotracheoscopy and the effects of using a lipid extract surfactant on the measurement.
Methodology
- The researchers used videotracheoscopy to study and measure the surface tension in the horse trachea.
- Small oil drops were placed on the tracheal wall using thin Teflon tubing inserted into a videocolonoscope typically used for humans.
- To compare results, 5 ml of saline were used in control experiments while 5 ml of Bovine Lipid Extract Surfactant (BLES) at a concentration of 4 mg/ml was used in test experiments.
Results
- The surface tension measured in the control experiments was found to be 31.9 mN/m.
- When BLES was administered, the surface tension in the tracheal wall significantly dropped to 24.5 mN/m.
- The researchers deduced these values from calibration curves that relate the film’s surface tension to the test fluid droplets’ relative diameters.
In Vitro Calibration Experiments
- The measurements were calibrated using test fluid droplets placed onto a surfactant film at various surface tensions.
- Two in vitro methods were used in the calibration: a modified Langmuir-Wilhelmy balance and a captive bubble surfactometer.
- The researchers observed that the model parameters, such as the spreading behavior of a given test fluid droplet, depended not only on the film’s surface tension but also on the thickness of the aqueous layer below the surfactant layer.
- Thus, it was concluded that the computed surface tensions in the trachea would rely greatly on the choice of which in vitro calibration method is employed.
Cite This Article
APA
Im Hof V, Gehr P, Gerber V, Lee MM, Schürch S.
(1997).
In vivo determination of surface tension in the horse trachea and in vitro model studies.
Respir Physiol, 109(1), 81-93.
https://doi.org/10.1016/s0034-5687(97)84032-7 Publication
Researcher Affiliations
- Department of Internal Medicine, University Hospital, Inselspital, Berne, Switzerland. vinzenz.im.hof@insel.ch
MeSH Terms
- Animals
- Horses
- In Vitro Techniques
- Models, Biological
- Respiratory Physiological Phenomena
- Surface Tension
- Trachea / physiology
Citations
This article has been cited 15 times.- Hages ND, Sembrat JC, Weber L, Johnston DJ, Stetten AZ, Sauleda M, Mulhern B, Tilton RD, Garoff S, Rojas M, Corcoran TE. Effect of a Surfactant Additive on Drug Transport and Distribution Uniformity After Aerosol Delivery to Ex Vivo Lungs.. J Aerosol Med Pulm Drug Deliv 2022 Jun;35(3):146-153.
- Iasella SV, Stetten AZ, Corcoran TE, Garoff S, Przybycien TM, Tilton RD. Aerosolizing Lipid Dispersions Enables Antibiotic Transport Across Mimics of the Lung Airway Surface Even in the Presence of Pre-existing Lipid Monolayers.. J Aerosol Med Pulm Drug Deliv 2018 Aug;31(4):212-220.
- Vasquez PA, Jin Y, Palmer E, Hill D, Forest MG. Modeling and Simulation of Mucus Flow in Human Bronchial Epithelial Cell Cultures - Part I: Idealized Axisymmetric Swirling Flow.. PLoS Comput Biol 2016 Aug;12(8):e1004872.
- Sharma R, Khanal A, Corcoran TE, Garoff S, Przybycien TM, Tilton RD. Surfactant Driven Post-Deposition Spreading of Aerosols on Complex Aqueous Subphases. 2: Low Deposition Flux Representative of Aerosol Delivery to Small Airways.. J Aerosol Med Pulm Drug Deliv 2015 Oct;28(5):394-405.
- Khanal A, Sharma R, Corcoran TE, Garoff S, Przybycien TM, Tilton RD. Surfactant Driven Post-Deposition Spreading of Aerosols on Complex Aqueous Subphases. 1: High Deposition Flux Representative of Aerosol Delivery to Large Airways.. J Aerosol Med Pulm Drug Deliv 2015 Oct;28(5):382-93.
- Farnoud AM, Fiegel J. Calf Lung Surfactant Recovers Surface Functionality After Exposure to Aerosols Containing Polymeric Particles.. J Aerosol Med Pulm Drug Deliv 2016 Feb;29(1):10-23.
- Hamed R, Fiegel J. Synthetic tracheal mucus with native rheological and surface tension properties.. J Biomed Mater Res A 2014 Jun;102(6):1788-98.
- Bartlett JA, Gakhar L, Penterman J, Singh PK, Mallampalli RK, Porter E, McCray PB Jr. PLUNC: a multifunctional surfactant of the airways.. Biochem Soc Trans 2011 Aug;39(4):1012-6.
- Gakhar L, Bartlett JA, Penterman J, Mizrachi D, Singh PK, Mallampalli RK, Ramaswamy S, McCray PB Jr. PLUNC is a novel airway surfactant protein with anti-biofilm activity.. PLoS One 2010 Feb 9;5(2):e9098.
- Rubin BK. The role of mucus in cough research.. Lung 2010 Jan;188 Suppl 1:S69-72.
- Alonso C, Waring A, Zasadzinski JA. Keeping lung surfactant where it belongs: protein regulation of two-dimensional viscosity.. Biophys J 2005 Jul;89(1):266-73.
- Edwards DA, Man JC, Brand P, Katstra JP, Sommerer K, Stone HA, Nardell E, Scheuch G. Inhaling to mitigate exhaled bioaerosols.. Proc Natl Acad Sci U S A 2004 Dec 14;101(50):17383-8.
- Alonso C, Alig T, Yoon J, Bringezu F, Warriner H, Zasadzinski JA. More than a monolayer: relating lung surfactant structure and mechanics to composition.. Biophys J 2004 Dec;87(6):4188-202.
- Geiser M, Matter M, Maye I, Im Hof V, Gehr P, Schürch S. Influence of airspace geometry and surfactant on the retention of man-made vitreous fibers (MMVF 10a).. Environ Health Perspect 2003 Jun;111(7):895-901.
- Tarran R, Grubb BR, Gatzy JT, Davis CW, Boucher RC. The relative roles of passive surface forces and active ion transport in the modulation of airway surface liquid volume and composition.. J Gen Physiol 2001 Aug;118(2):223-36.
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