In vivo effects of an intrafollicular injection of insulin-like growth factor 1 on the mechanism of follicle deviation in heifers and mares.
Abstract: In cattle and mares, free insulin-like growth factor 1 (IGF-1) is higher in the future dominant follicle (F1) than in the future largest subordinate follicle (F2) before deviation in diameter or selection is manifested between the two follicles. The effect of IGF-1 on other follicular-fluid factors and on the destiny of F2 were studied in two experiments in each species, using a total of 40 heifers and 42 mares. An injection of IGF-1 was made into F2 at the expected beginning of deviation (heifers, F1 >or= 8.5 mm; mares, F1 >or= 20.0 mm; Hour 0). In heifers, follicular fluid was taken from F2 at Hours 3, 6, 12, or 24; each heifer was sampled only once. In mares, sequential F2 samples were taken from each mare at Hours 0, 6, and 24 or at Hours 12 and 24. Transvaginal ultrasound guidance was used for treatment and sample collection. In heifers, IGF-1 treatment of F2 stimulated the secretion of estradiol (P < 0.05) between Hours 3 and 6 and androstenedione (P < 0.05) between Hours 3 and 12. In F2 of control heifers, estradiol decreased (P < 0.05) and androstenedione did not change significantly. In mares, IGF-1 treatment of F2 did not affect the concentrations of estradiol during the 24-h posttreatment period; androstenedione decreased (P < 0.04) in the IGF-1 group and increased (P < 0.006) in the controls. Compared with control mares, the IGF-1 group had higher (P < 0.04) activin-A at Hours 12 and 24 and higher (P < 0.0006) inhibin-A at Hour 24. After ablating F1 at Hour 24 in mares, F2 became dominant and ovulated in more mares (P < 0.0002) in the IGF-1 group (12/14) than in the control group (2/14). These results are consistent with reported temporal relationships among follicular factors during deviation in both species and indicate that IGF-1 plays a key role in controlling the temporal relationships; however, no indication was found that IGF-1 stimulated estradiol production in mares during the 24 h after treatment.
Publication Date: 2003-09-03 PubMed ID: 12954722DOI: 10.1095/biolreprod.103.021949Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research examined how insulin-like growth factor 1 (IGF-1) works within follicles in cattle and mares. It showed that injecting IGF-1 into the subordinate follicle can redirect follicular development, leading to increased production of hormones and dominance among subordinate follicles, however, no such stimulation was found in mares.
The Experiment
- The researchers made use of a total of 82 animals; 40 heifers (young cows) and 42 mares (female horses).
- They studied the amount of free IGF-1 within two types of ovarian follicles, the dominant (F1) and largest subordinate (F2) follicles.
- An injection of IGF-1 was made into F2 follicles at the expected starting point of follicular deviation, defined as when F1 was larger than 8.5mm in heifers or 20.0mm in mares.
Data Collection
- The collected follicular fluid from F2 at varying intervals after IGF-1 injection, specifically at 3, 6, 12, and 24 hours, with each animal being sampled only once.
- In mares, sequential F2 samples were collected at 0, 6, and 24 hours, or at 12 and 24 hours.
Results for Heifers
- The results found that in heifers, IGF-1 treated F2 follicles showed increased estradiol secretion between 3 and 6 hours and androstenedione secretion between 3 and 12 hours.
- Conversely, in untreated heifers, estradiol levels decreased and androstenedione levels remained largely unchanged.
Results for Mares
- In mares, IGF-1 treatment of F2 follicles did not have a significant effect on the concentrations of estradiol during the 24-hour post-treatment period.
- Androstenedione concentrations did however decrease in treated mares, but increased in untreated ones.
- Following the removal of the prospective dominant (F1) follicle at 24 hours, the IGF-1 injected subordinate follicles (F2) assumed dominance and ovulated more frequently than those in the untreated group.
Conclusion
- This indicates that IGF-1 likely plays a significant role in follicular deviation, as it influences the hormonal outputs within subordinate follicles.
- However, it’s also important to note that the effects were observed to be inconsistent between the two species, specifically with estradiol production in mares seemingly unaffected.
Cite This Article
APA
Ginther OJ, Bergfelt DR, Beg MA, Meira C, Kot K.
(2003).
In vivo effects of an intrafollicular injection of insulin-like growth factor 1 on the mechanism of follicle deviation in heifers and mares.
Biol Reprod, 70(1), 99-105.
https://doi.org/10.1095/biolreprod.103.021949 Publication
Researcher Affiliations
- Eutheria Foundation, Cross Plains, Wisconsin 53528, USA. ginther@svm.vetmed.wisc.edu
MeSH Terms
- Androstenediol / metabolism
- Animals
- Cattle
- Estradiol / metabolism
- Female
- Follicular Fluid / metabolism
- Horses
- Insulin-Like Growth Factor I / pharmacology
- Ovarian Follicle / diagnostic imaging
- Ovarian Follicle / drug effects
- Ovarian Follicle / metabolism
- Ovulation / drug effects
- Progesterone / metabolism
- Ultrasonography
Citations
This article has been cited 10 times.- Gebremedhn S, Gad A, Ishak GM, Menjivar NG, Gastal MO, Feugang JM, Prochazka R, Tesfaye D, Gastal EL. Dynamics of extracellular vesicle-coupled microRNAs in equine follicular fluid associated with follicle selection and ovulation. Mol Hum Reprod 2023 Apr 3;29(4).
- Garcia-Guerra A, Wiltbank MC, Battista SE, Kirkpatrick BW, Sartori R. Mechanisms regulating follicle selection in ruminants: lessons learned from multiple ovulation models. Anim Reprod 2018 Jul-Sep;15(Suppl 1):660-679.
- Malard PF, Peixer MAS, Grazia JG, Brunel HDSS, Feres LF, Villarroel CL, Siqueira LGB, Dode MAN, Pogue R, Viana JHM, Carvalho JL. Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. Sci Rep 2020 May 15;10(1):8018.
- Sharma A, Baddela VS, Becker F, Dannenberger D, Viergutz T, Vanselow J. Elevated free fatty acids affect bovine granulosa cell function: a molecular cue for compromised reproduction during negative energy balance. Endocr Connect 2019 May 1;8(5):493-505.
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- Jung SY, Willard ST. Quantitative bioluminescence imaging of transgene expression in intact porcine antral follicles in vitro. Reprod Biol Endocrinol 2014 Jan 30;12:11.
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- Rao JU, Shah KB, Puttaiah J, Rudraiah M. Gene expression profiling of preovulatory follicle in the buffalo cow: effects of increased IGF-I concentration on periovulatory events. PLoS One 2011;6(6):e20754.
- Rawan AF, Langar H, Munetomo M, Yamamoto Y, Kawano K, Kimura K. Effects of insulin-like growth factor-1 on the mRNA expression of estradiol receptors, steroidogenic enzymes, and steroid production in bovine follicles. J Reprod Dev 2023 Dec 8;69(6):337-346.
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