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Inability of FMDV replication in equine kidney epithelial cells is independent of integrin αvβ3 and αvβ6.
Abstract: Integrins can function as receptors for foot-and-mouth disease virus (FMDV) in epithelium. Horses are believed to be insusceptible to this disease, but the mechanism of resistance remains unclear. To detect whether FMDV can use integrin to attach to equine epithelial, we compared the utilities of αvβ3 and αvβ6 between bovine and equine kidney epithelial cells (KECs). Equine KECs showed almost equal efficiency to those of bovine. Further, the integrin αv, β3, and β6 subunits from bovine and equine were cloned and vectors were transfected into SW480 cells and COS-1 cells alone or together, and virus titers were used to determine the viral replication. In all cases, the virus reproduced successfully. Overall, FMDV can replicate in SW480 cells transfected with equine β3/β6 subunits and COS-1 cells transfected with equine αvβ3/αvβ6 integrins, but not in EKECs. These results indicated that failure of FMDV replication in EKECs was not attributed to integrin receptors.
Copyright © 2016. Published by Elsevier Inc.
Publication Date: 2016-03-21 PubMed ID: 27011223DOI: 10.1016/j.virol.2016.01.025Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research explores why horses appear to be impervious to foot-and-mouth disease virus (FMDV), finding that the failure of the virus to replicate in horse kidney cells is not due to the interaction with specific integrin receptors.
Introduction to the Study
- The research focuses on understanding why horses remain unaffected by the foot-and-mouth disease virus (FMDV). The virus is known to affect the epithelium and uses integrins as receptors.
- The study aimed to determine if the resistance in horses was due to the virus’s inability to utilize horse integrin receptors to attach to kidney epithelial cells.
Methodology
- The research compared the performance of two types of integrin receptors (αvβ3 and αvβ6) in horse and cow kidney epithelial cells.
- In order to examine the effect of these integrins, the subunits αv, β3, and β6 from bovine and equine were cloned and vectors were then injected into SW480 cells and COS-1 cells. Virus titers were then used to evaluate the level of viral replication.
Findings
- The study results showed that the FMDV was able to successfully reproduce in the SW480 cells with the horse β3/β6 subunits and in the COS-1 cells with the horse αvβ3/αvβ6 integrins.
- However, in EKECs (Equine Kidney Epithelial Cells), the virus was not capable of reproducing. This suggests that the absence of FMDV replication in horse kidney cells was not due to the interaction with the integrin receptors.
Conclusions
- This study helped to ascertain that the immunity of horses to FMD virus is not due to the virus’s failure to interact with horse integrin receptors.
- Therefore, the exact mechanism behind the horse’s resistance to FMDV still remains unclear and calls for more research.
Cite This Article
APA
Wang Y, Mao Q, Chang H, Wu Y, Pan S, Li Y, Zhang Y.
(2016).
Inability of FMDV replication in equine kidney epithelial cells is independent of integrin αvβ3 and αvβ6.
Virology, 492, 251-258.
https://doi.org/10.1016/j.virol.2016.01.025 Publication
Researcher Affiliations
- College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, PR China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
- College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, PR China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
- State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046 Gansu, PR China.
- College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, PR China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
- College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, PR China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
- College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, PR China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
- College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, PR China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, PR China. Electronic address: zhy1956@263.net.
MeSH Terms
- Animals
- Antigens, Neoplasm / genetics
- Antigens, Neoplasm / immunology
- COS Cells
- Cattle
- Chlorocebus aethiops
- Cloning, Molecular
- Cricetulus
- Disease Resistance
- Epithelial Cells / immunology
- Epithelial Cells / pathology
- Epithelial Cells / virology
- Foot-and-Mouth Disease Virus / genetics
- Foot-and-Mouth Disease Virus / growth & development
- Gene Expression
- Horses
- Host Specificity
- Integrin alphaVbeta3 / genetics
- Integrin alphaVbeta3 / immunology
- Integrins / genetics
- Integrins / immunology
- Kidney / immunology
- Kidney / pathology
- Kidney / virology
- Receptors, Virus / genetics
- Receptors, Virus / immunology
- Recombinant Proteins / genetics
- Recombinant Proteins / immunology
- Transgenes
- Virus Replication
Citations
This article has been cited 4 times.- Li K, Wang C, Yang F, Cao W, Zhu Z, Zheng H. Virus-Host Interactions in Foot-and-Mouth Disease Virus Infection. Front Immunol 2021;12:571509.
- Ren M, Zhou H, Wu J-e, Wang J-n, Wang X, Abdullah SW, Guo H, Sun S. Heat shock protein A1 inhibits the replication of foot-and-mouth disease virus by degrading viral RNA polymerase 3D through chaperone-mediated autophagy. J Virol 2025 May 20;99(5):e0016825.
- Gaboiphiwe K, Kabelo TI, Mosholombe PT, Hyera J, Fana EM, Masisi K, Lebani K. A Review of the Utility of Established Cell Lines for Isolation and Propagation of the Southern African Territories Serotypes of Foot-and-Mouth Disease Virus. Viruses 2024 Dec 30;17(1).
- Zewdie G, Akalu M, Tolossa W, Belay H, Deresse G, Zekarias M, Tesfaye Y. A review of foot-and-mouth disease in Ethiopia: epidemiological aspects, economic implications, and control strategies. Virol J 2023 Dec 15;20(1):299.
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