Incidence of cutaneous habronemosis in Manipuri ponies in India.
Abstract: Information pertaining to parasitic fauna and parasitic diseases in Manipuri ponies in India is not available. Moreover, no systematic studies have been undertaken on cutaneous habronemosis in Manipuri ponies which is a common skin problem of Manipuri ponies as reported by pony owners. Keeping in the view of the importance of parasitic infections in veterinary health coverage particularly in Manipuri ponies, the present study was planned. A survey of natural cases of cutaneous habronemosis followed by molecular confirmation of species involved and treatments were done. Out of 200 ponies examined, nine cases (4.5%) of cutaneous habronemosis was recorded. Gross examination revealed raised and ulcerated wounds with necrotic tissues covered with yellowish-tan granulation. Histopathological study revealed eosinophilic granuloma and in the center of the granuloma with necrotic debris. Remnants of the Hebronema larvae with infiltrating neutrophils surrounded by proliferating fibrous tissue with numerous eosinophils, macrophages and lymphocytes were also observed. Molecular detection of Habronema sp. was confirmed by semi-nested PCR. Sequence analysis revealed larvae of H. muscae was the common spirurid species responsible for producing cutaneous habronemosis in Manipuri ponies. Subsequently, sequence submitted to NCBI GenBank and accession number obtained (MH038181). Surgical removal of necrotic tissue, ivermectin injection along with antibiotics successfully cured all the lesions in infected ponies.Results confirmed occurrence of cutaneous habronemosis in Manipuri ponies in India.
Copyright © 2019 Elsevier B.V. All rights reserved.
Publication Date: 2019-04-24 PubMed ID: 31303226DOI: 10.1016/j.vprsr.2019.100295Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article discusses the incidence of cutaneous habronemosis, a parasitic skin disease, in Manipuri ponies in India and the effectiveness of certain treatments in curing the lesions caused by the disease.
Objective and Methods
- The researchers aimed to explore the incidence and particulars of cutaneous habronemosis in Manipuri ponies, a topic that has not been systematically studied before. This disease is frequently reported among these ponies by their owners.
- For the study, a survey was conducted on naturally occurring cases of cutaneous habronemosis, followed by molecular confirmation of the species involved and treatments of the affected ponies.
- A sample of 200 ponies were examined, which resulted in the identification of nine cases of cutaneous habronemosis, implying a rate of 4.5%.
Findings
- Upon physical examination, raised and ulcerated wounds with necrotic tissues covered with a yellowish-tan granulation were observed.
- A histopathological examination revealed eosinophilic granuloma, a type of inflammation, and presence of necrotic debris in the center of the granuloma.
- Parts of Hebronema larvae were found surrounded by proliferating fibrous tissue with numerous eosinophils, macrophages, and lymphocytes – indicating an active immune response.
Molecular Detection and Analysis
- The parasitic organism was confirmed to be of the Habronema species through semi-nested PCR, a molecular detection technique.
- Upon sequencing analysis, the larvae were found to belong to H. muscae, a spirurid species commonly responsible for cutaneous habronemosis.
- The sequence was submitted to the NCBI GenBank, a genetic sequence database, and it was given an accession number MH038181.
Treatment and Conclusion
- Employing surgical removal of necrotic tissue, ivermectin injections and antibiotics successfully cured all the lesions caused by the disease in the infected ponies.
- The study confirms the occurrence of cutaneous habronemosis in the Manipuri ponies in India.
Cite This Article
APA
Devi CN, Borthakur SK, Patra G, Singh NS, Tolenkhomba TC, Ravindran R, Ghosh S.
(2019).
Incidence of cutaneous habronemosis in Manipuri ponies in India.
Vet Parasitol Reg Stud Reports, 17, 100295.
https://doi.org/10.1016/j.vprsr.2019.100295 Publication
Researcher Affiliations
- Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India.
- Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India. Electronic address: sanjoy_barthakur@rediffmail.com.
- Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India.
- Department of Animal Genetics and Breeding, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India.
- Department of Animal Genetics and Breeding, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India.
- Department of Veterinary Pathology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India.
- Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India.
MeSH Terms
- Amoxicillin-Potassium Clavulanate Combination / administration & dosage
- Animals
- Antiparasitic Agents / administration & dosage
- Base Sequence
- Biopsy / veterinary
- Cloning, Molecular
- DNA, Helminth / chemistry
- DNA, Helminth / isolation & purification
- DNA, Ribosomal / chemistry
- DNA, Ribosomal / genetics
- Eosinophilic Granuloma / parasitology
- Eosinophilic Granuloma / pathology
- Eosinophilic Granuloma / veterinary
- Feces / parasitology
- Female
- Horse Diseases / epidemiology
- Horse Diseases / parasitology
- Horse Diseases / pathology
- Horse Diseases / therapy
- Horses
- Incidence
- India / epidemiology
- Ivermectin / administration & dosage
- Male
- Necrosis / veterinary
- Polymerase Chain Reaction
- Sequence Alignment / veterinary
- Skin Diseases, Parasitic / epidemiology
- Skin Diseases, Parasitic / parasitology
- Skin Diseases, Parasitic / pathology
- Skin Diseases, Parasitic / veterinary
- Spirurida Infections / epidemiology
- Spirurida Infections / parasitology
- Spirurida Infections / pathology
- Spirurida Infections / veterinary
- Spiruroidea / genetics
- Spiruroidea / isolation & purification
Citations
This article has been cited 3 times.- Salem MA, El-Gameel SM, Kamel MS, Elsamman EM, Ramadan RM. Innovative diagnostic strategies for equine habronemiasis: exploring molecular identification, gene expression, and oxidative stress markers. Parasit Vectors 2025 Aug 2;18(1):325.
- Américo L, Aquino LPCT, Moura AB, Ribeiro GSN, Fonteque JH, Chryssafidis AL. Cutaneous and conjunctival habronemosis in horses treated at the Veterinary Hospital of the Santa Catarina State University, Brazil. Rev Bras Parasitol Vet 2024;33(3):e004224.
- Palozzo A, Traversa D, Marruchella G, Celani G, Morelli S, Petrizzi L. Summer Sores Secondary to a Hoof Crack in an Andalusian Stallion. Pathogens 2021 Aug 16;10(8).
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