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The Histochemical journal1986; 18(1); 1-4; doi: 10.1007/BF01676191

Increased acidophilia of eosinophil granules after EDTA treatment.

Abstract: The acidophilic reaction of eosinophil leucocyte granules from human, pig and horse blood smears was investigated by using May-Grünwald-Giemsa staining after previous treatment with EDTA and sodium citrate solutions. The same peak at 530 nm, but absorption values considerably higher than those of controls, were found in eosinophil granules after application of chelating agents, indicating that removal of metal cations could unmask basic groups in these structures.
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Publication Date: 1986-01-01 PubMed ID: 3086258DOI: 10.1007/BF01676191Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the impact of EDTA (a chelating agent) on the reaction of eosinophil leucocyte granules (a type of white blood cell) from blood samples of humans, pigs, and horses. The findings suggest that the removal of metal cations through the use of EDTA results in higher absorption values compared to controls, which implies the involvement of basic groups in these cell structures.

Research Objective and Approach

  • This research aimed to investigate the role of chelating agents, specifically EDTA (Ethylenediaminetetraacetic acid) and sodium citrate, on the acidophilic reaction of eosinophil leucocyte granules.
  • The researchers used May-Grünwald-Giemsa staining, a common technique used for blood smear staining, to observe the changes in granules after treatment with these agents.
  • Blood smears were taken from humans, pigs, and horses to ensure a broader investigation and applicability of results.

Findings and Interpretation

  • It was found that after applying chelating agents, the eosinophil granules displayed the same peak at 530 nm but had considerably higher absorption values when compared with controls. The peak’s wavelength refers to the absorbance of light, indicating the granules’ reaction to the treatment.
  • The increase in absorption values implies that the chelating agents were able to remove metal cations, unmasking basic groups within these structures. Metal cations are positively charged particles; their removal may unveil previously hidden or inaccessible components of the granules, leading to higher absorption.

Implications of the Study

  • The findings suggest a possible mechanism of eosinophil granule reaction involving basic groups. These basic groups may play a key role in how these blood cells react and function.
  • This research provides additional insight into the internal structures and functionalities of eosinophil leucocytes, potentially helping with understanding diseases that involve these cells like allergies and asthma.
  • Further research can utilize these findings in developing more effective treatment methods for conditions influenced by these cells.

Cite This Article

APA
Gomez-Perretta C, Armas-Portela R, Stockert JC, Tato A, Ferrer JM. (1986). Increased acidophilia of eosinophil granules after EDTA treatment. Histochem J, 18(1), 1-4. https://doi.org/10.1007/BF01676191

Publication

The Histochemical journal
ISSN: 0018-2214
NlmUniqueID: 0163161
Country: Netherlands
Language: English
Volume: 18
Issue: 1
Pages: 1-4

Researcher Affiliations

Gomez-Perretta, C
    Armas-Portela, R
      Stockert, J C
        Tato, A
          Ferrer, J M

            MeSH Terms

            • Animals
            • Azure Stains
            • Cytophotometry
            • Cytoplasmic Granules / analysis
            • Edetic Acid
            • Eosinophils / analysis
            • Horses
            • Humans
            • Hydrogen-Ion Concentration
            • Phenothiazines
            • Swine

            References

            This article includes 14 references
            1. Sissoëff I, Grisvard J, Guillé E. Studies on metal ions-DNA interactions: specific behaviour of reiterative DNA sequences.. Prog Biophys Mol Biol 1976;31(2):165-99.
              pubmed: 185659doi: 10.1016/0079-6107(78)90008-1google scholar: lookup
            2. Yokota S, Deimann W, Hashimoto T, Fahimi HD. Immunocytochemical localization of two peroxisomal enzymes of lipid beta-oxidation in specific granules of rat eosinophils.. Histochemistry 1983;78(4):425-33.
              pubmed: 6352572doi: 10.1007/BF00496194google scholar: lookup
            3. Wittekind DH. On the nature of Romanowsky--Giemsa staining and its significance for cytochemistry and histochemistry: an overall view.. Histochem J 1983 Oct;15(10):1029-47.
              pubmed: 6196323doi: 10.1007/BF01002498google scholar: lookup
            4. Yokota S, Tsuji H, Kato K. Localization of lysosomal and peroxisomal enzymes in the specific granules of rat intestinal eosinophil leukocytes revealed by immunoelectron microscopic techniques.. J Histochem Cytochem 1984 Mar;32(3):267-74.
              pubmed: 6693757doi: 10.1177/32.3.6693757google scholar: lookup
            5. Wittekind DH, Gehring T. On the nature of Romanowsky-Giemsa staining and the Romanowsky-Giemsa effect. I. Model experiments on the specificity of azure B-eosin Y stain as compared with other thiazine dye-eosin Y combinations.. Histochem J 1985 Mar;17(3):263-89.
              pubmed: 2411682doi: 10.1007/BF01004591google scholar: lookup
            6. Stockert JC, Colman OD, Cañete M. Fluorescence reaction of leukocyte granules by morin.. Acta Histochem Suppl 1985;31:243-52.
              pubmed: 2410949
            7. ARCHER GT, HIRSCH JG. ISOLATION OF GRANULES FROM EOSINOPHIL LEUCOCYTES AND STUDY OF THEIR ENZYME CONTENT.. J Exp Med 1963 Aug 1;118(2):277-86.
              pubmed: 14074391doi: 10.1084/jem.118.2.277google scholar: lookup
            8. Ackerman SJ, Kephart GM, Habermann TM, Greipp PR, Gleich GJ. Localization of eosinophil granule major basic protein in human basophils.. J Exp Med 1983 Sep 1;158(3):946-61.
              pubmed: 6350526doi: 10.1084/jem.158.3.946google scholar: lookup
            9. Sumner AT, Evans HJ. Mechanisms involved in the banding of chromosomes with guinacrine and Giems. II. The interaction of the dyes with the chromosomal components.. Exp Cell Res 1973 Sep;81(1):223-6.
              pubmed: 4127952doi: 10.1016/0014-4827(73)90129-8google scholar: lookup
            10. Bernhard W. A new staining procedure for electron microscopical cytology.. J Ultrastruct Res 1969 May;27(3):250-65.
              pubmed: 4181256doi: 10.1016/s0022-5320(69)80016-xgoogle scholar: lookup
            11. Friedberg F. Effects of metal binding on protein structure.. Q Rev Biophys 1974 Feb;7(1):1-33.
              pubmed: 4600801doi: 10.1017/s0033583500001335google scholar: lookup
            12. Stockert JC. Uranyl-EDTA-hematoxylin: a new selective staining technique for nucleolar material.. Histochemistry 1975 Jun 9;43(4):313-22.
              pubmed: 52633doi: 10.1007/BF00490190google scholar: lookup
            13. Zipfel E, Grezes JR, Seiffert W, Zimmermann HW. [Romanowsky dyes and Romanowsky-Giemsa effect. 2. Eosin Y, erythrosin B, tetrachlorofluorescein, spectroscopic characterization of pure dyes, association of eosin Y].. Histochemistry 1982;75(4):539-55.
              pubmed: 6184340
            14. Galbraith W, Marshall PN, Bacus JW. Microspectrophotometric studies of Romanowsky stained blood cells. I. Subtraction analysis of a standardized procedure.. J Microsc 1980 Aug;119(3):313-30.
              pubmed: 6157817doi: 10.1111/j.1365-2818.1980.tb04104.xgoogle scholar: lookup

            Citations

            This article has been cited 1 times.
            1. Trigoso CI, Espada J, Stockert JC. Fluorescence of eosinophil leucocyte granules induced by 1-hydroxy-3,6,8-pyrenetrisulfonate. Visualization of differences in protein isoelectric points. Histochem Cell Biol 1995 Jul;104(1):69-73.
              doi: 10.1007/BF01464788pubmed: 7584562google scholar: lookup
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