Increased acidophilia of eosinophil granules after EDTA treatment.
Abstract: The acidophilic reaction of eosinophil leucocyte granules from human, pig and horse blood smears was investigated by using May-Grünwald-Giemsa staining after previous treatment with EDTA and sodium citrate solutions. The same peak at 530 nm, but absorption values considerably higher than those of controls, were found in eosinophil granules after application of chelating agents, indicating that removal of metal cations could unmask basic groups in these structures.
Publication Date: 1986-01-01 PubMed ID: 3086258DOI: 10.1007/BF01676191Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article investigates the impact of EDTA (a chelating agent) on the reaction of eosinophil leucocyte granules (a type of white blood cell) from blood samples of humans, pigs, and horses. The findings suggest that the removal of metal cations through the use of EDTA results in higher absorption values compared to controls, which implies the involvement of basic groups in these cell structures.
Research Objective and Approach
- This research aimed to investigate the role of chelating agents, specifically EDTA (Ethylenediaminetetraacetic acid) and sodium citrate, on the acidophilic reaction of eosinophil leucocyte granules.
- The researchers used May-Grünwald-Giemsa staining, a common technique used for blood smear staining, to observe the changes in granules after treatment with these agents.
- Blood smears were taken from humans, pigs, and horses to ensure a broader investigation and applicability of results.
Findings and Interpretation
- It was found that after applying chelating agents, the eosinophil granules displayed the same peak at 530 nm but had considerably higher absorption values when compared with controls. The peak’s wavelength refers to the absorbance of light, indicating the granules’ reaction to the treatment.
- The increase in absorption values implies that the chelating agents were able to remove metal cations, unmasking basic groups within these structures. Metal cations are positively charged particles; their removal may unveil previously hidden or inaccessible components of the granules, leading to higher absorption.
Implications of the Study
- The findings suggest a possible mechanism of eosinophil granule reaction involving basic groups. These basic groups may play a key role in how these blood cells react and function.
- This research provides additional insight into the internal structures and functionalities of eosinophil leucocytes, potentially helping with understanding diseases that involve these cells like allergies and asthma.
- Further research can utilize these findings in developing more effective treatment methods for conditions influenced by these cells.
Cite This Article
APA
Gomez-Perretta C, Armas-Portela R, Stockert JC, Tato A, Ferrer JM.
(1986).
Increased acidophilia of eosinophil granules after EDTA treatment.
Histochem J, 18(1), 1-4.
https://doi.org/10.1007/BF01676191 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Azure Stains
- Cytophotometry
- Cytoplasmic Granules / analysis
- Edetic Acid
- Eosinophils / analysis
- Horses
- Humans
- Hydrogen-Ion Concentration
- Phenothiazines
- Swine
References
This article includes 14 references
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Citations
This article has been cited 1 times.- Trigoso CI, Espada J, Stockert JC. Fluorescence of eosinophil leucocyte granules induced by 1-hydroxy-3,6,8-pyrenetrisulfonate. Visualization of differences in protein isoelectric points. Histochem Cell Biol 1995 Jul;104(1):69-73.
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