Induction of double ovulation in mares using deslorelin acetate.
Abstract: This study aimed to determine whether deslorelin acetate could induce double ovulation in mares. In Experiment 1, eight mares were treated with prostaglandin on Day 8 (D8) after ovulation, then treated with saline or with 100 μg of a controlled-release formulation of deslorelin acetate vehicle intramuscularly (IM) every 12h from D8 after ovulation until at least two follicles reached 33 mm. At this time, ovulation was induced with 2500 IU of hCG. Artificial insemination was performed 24h after induction, and embryos were collected on the eighth day after ovulation was first detected. In Experiment 2, 112 estrous cycles in 56 mares were studied. In this experiment, the deslorelin acetate protocol was initiated only in mares that achieved a follicle with a diameter of at least 25 mm and at least one second follicle with a diameter≥20mm was detected, at which time 100 μg deslorelin acetate or saline was administered IM every 12h. The other procedures were similar to those described in Experiment 1. The variables studied were analyzed using Student's t-test and Fisher's exact test. In Experiment 1, only two mares in deslorelin group having second follicles of 20-25 mm on responded with double ovulation. In the second experiment, 82% of treated mares responded with double ovulation, and the embryo recovery per estrous cycle was 1.12 and 0.57 in the group treated with deslorelin acetate and the control group, respectively (P<0.05). Deslorelin acetate is effective in inducing double ovulation in mares using the protocol proposed. On average, it allows for the recovery of one embryo by uterine flushing.
Copyright © 2012 Elsevier B.V. All rights reserved.
Publication Date: 2012-11-01 PubMed ID: 23182475DOI: 10.1016/j.anireprosci.2012.10.015Google Scholar: Lookup
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- Journal Article
- Randomized Controlled Trial
- Research Support
- Non-U.S. Gov't
Summary
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The research investigates if deslorelin acetate can be used to induce double ovulation in horses. The results showed that deslorelin acetate is effective in causing double ovulation and allows for the recovery of one embryo by uterine flushing.
Study Design and Methodology
- The study was conducted in two phases, each involving a different number of mares and altering the conditions for initiating the deslorelin acetate protocol.
- In the first phase, eight mares were treated with a controlled-release formulation of deslorelin acetate or a saline solution every 12 hours after the initial ovulation had occurred, until at least two follicles enlarged to 33 mm.
- Upon reaching this size, ovulation was induced using 2500 IU of human chorionic gonadotropin (hCG), a hormone that stimulates the production and release of eggs from the ovaries. Artificial insemination was followed, and embryos were collected eight days later.
- The second phase of the experiment involved 56 mares and 112 estrous cycles. It followed the same procedure as the first phase but only initiated the deslorelin acetate protocol when mares already had a follicle with a diameter of at least 25 mm and a second follicle of at least 20 mm.
- Statistical analysis was performed on the collected data using Student’s t-test and Fisher’s exact test.
Results and Conclusion
- In the first phase of the experiment, only two mares responded with double ovulation when deslorelin acetate was used.
- In contrast, the second phase’s results showed an 82% response rate with double ovulation in the group given deslorelin acetate.
- The average embryo recovery per estrous cycle was also significantly higher in the group treated with this substance compared to the control group.
- Based on these results, the research concludes that deslorelin acetate can be beneficial for inducing double ovulation in mares, considerably increasing the chances of embryo retrieval following artificial insemination.
- The article suggests that this method could play a crucial role in the reproductive management of horses, including in the thoroughbred industry.
Cite This Article
APA
Nagao JF, Neves Neto JR, Papa FO, Alvarenga MA, Freitas-Dell'Aqua CP, Dell'Aqua JA.
(2012).
Induction of double ovulation in mares using deslorelin acetate.
Anim Reprod Sci, 136(1-2), 69-73.
https://doi.org/10.1016/j.anireprosci.2012.10.015 Publication
Researcher Affiliations
- College of Veterinary Medicine e Animal Science, Department of Animal Reproduction and Veterinary Radiology, UNESP University of Sao Paulo State, Botucatu, SP. Brazil.
MeSH Terms
- Animals
- Embryo Transfer / veterinary
- Enzyme Inhibitors / pharmacology
- Female
- Horses / physiology
- Ovarian Follicle / drug effects
- Ovarian Follicle / physiology
- Ovulation / drug effects
- Ovulation Induction / methods
- Ovulation Induction / veterinary
- Triptorelin Pamoate / analogs & derivatives
- Triptorelin Pamoate / pharmacology
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