English
French
Inflammatory stimuli differentially modulate the transcription of paracrine signaling molecules of equine bone marrow multipotent mesenchymal stromal cells.
Abstract: Osteoarthritis (OA) is a degenerative disease of joint tissues that causes articular cartilage erosion, osteophytosis and loss of function due to pain. Inflammation and inflammatory cytokines in synovial fluid (SF) contribute to OA progression. Intra-articular (IA) injections of multipotent mesenchymal stromal cells (MSCs) are employed to treat OA in both humans and animals. MSCs secrete paracrine pro-inflammatory and anabolic signaling molecules that promote tissue repair. The objective of this study was to investigate the effects of OASF on the gene expression of paracrine signaling molecules by MSCs. Methods: The effects of Lipopolysaccharide (LPS) and interleukin (IL)-1β as well as both normal (N) and osteoarthritis (OA) SF stimulations on the expression of paracrine pro-inflammatory (tumor necrosis factor (TNF)-α, IL-1β, IL-8), modulatory (IL-6) and anabolic (vascular endothelial growth factor (VEGF), transforming growth factor (TGF)-β1 and insulin-like growth factor (IGF)-1) signaling molecules by equine bone marrow multipotent mesenchymal stromal cells (eBM-MSCs) was investigated employing reverse transcriptase-polymerase chain reaction (RT-PCR). Results: In contrast with NSF, OASF significantly up-regulated the expression of VEGF in eBM-MSCs. Both NSF and OASF significantly down-regulated the expression of IL-1β. LPS and IL-1β significantly increased the expression of pro-inflammatory cytokines (TNF-α, IL-8 and IL-6; and IL-1β and IL-8 respectively). Conclusions: We conclude that the transcription of paracrine signaling molecules in eBM-MSCs is modulated by SF. Furthermore, OA alters the properties of SF and the response of eBM-MSCs. Finally, the effects of LPS or IL-1β stimulation are distinct to that observed following stimulations with OASF.
Copyright © 2013 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
Publication Date: 2013-05-14 PubMed ID: 23685224DOI: 10.1016/j.joca.2013.05.004Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research investigates how inflammatory stimuli affect the gene expression of signaling molecules in bone marrow multipotent mesenchymal stromal cells. Key findings reveal significant up-regulation of certain molecules due to synovial fluid from osteoarthritis patients, highlighting the role of this fluid in disease progression and potential approaches to treatment.
Objectives and Methods
- The study aimed to explore the impact of the osteoarthritis synovial fluid (OASF) on the gene expression of paracrine signaling molecules produced by multipotent mesenchymal stromal cells (MSCs)
- The researchers used Lipopolysaccharide (LPS) and Interleukin-1β, along with both normal (N) and osteoarthritis (OA) synovial fluids to stimulate the MSCs
- The expression of pro-inflammatory, modulatory, and anabolic signaling molecules was then investigated using reverse transcriptase-polymerase chain reaction (RT-PCR)
Key Findings
- OASF significantly increased the expression of vascular endothelial growth factor (VEGF) in equine bone marrow multipotent mesenchymal stromal cells (eBM-MSCs)
- Both N and OASF synovial fluids led to a significant decrease in the expression of interleukin-1β, a pro-inflammatory cytokine
- LPS and interleukin-1β notably raised the production of pro-inflammatory cytokines, namely tumor necrosis factor-α, interleukin-8, and interleukin-6
Conclusions
- The study concludes that synovial fluid can alter the transcription of paracrine signaling molecules in eBM-MSCs, pointing out different responses to OASF compared to other inflammatory stimuli
- The findings suggest that osteoarthritis changes the properties of the synovial fluid, which further impacts the response of eBM-MSCs
- The effects following inflammatory stimuli using LPS or interleukin-1β are distinct from those observed when using OASF
- These findings have potential implications for the development of therapeutic approaches to OA, by manipulating the paracrine signaling molecules of MSCs
Cite This Article
APA
Vézina Audette R, Lavoie-Lamoureux A, Lavoie JP, Laverty S.
(2013).
Inflammatory stimuli differentially modulate the transcription of paracrine signaling molecules of equine bone marrow multipotent mesenchymal stromal cells.
Osteoarthritis Cartilage, 21(8), 1116-1124.
https://doi.org/10.1016/j.joca.2013.05.004 Publication
Researcher Affiliations
- Comparative Orthopedic Research Laboratory, Département de sciences cliniques, Faculté de Médecine vétérinaire, Université de Montréal, St Hyacinthe, QC, Canada.
MeSH Terms
- Animals
- Cells, Cultured
- Gene Expression Regulation / drug effects
- Horse Diseases / metabolism
- Horse Diseases / pathology
- Horses
- Inflammation Mediators / metabolism
- Inflammation Mediators / pharmacology
- Intercellular Signaling Peptides and Proteins / biosynthesis
- Intercellular Signaling Peptides and Proteins / genetics
- Interleukin-1beta / pharmacology
- Lipopolysaccharides / pharmacology
- Mesenchymal Stem Cells / drug effects
- Mesenchymal Stem Cells / metabolism
- Mesenchymal Stem Cells / physiology
- Osteoarthritis / metabolism
- Osteoarthritis / pathology
- Osteoarthritis / veterinary
- Paracrine Communication / drug effects
- Paracrine Communication / genetics
- Synovial Fluid / chemistry
- Transcription, Genetic / drug effects
Citations
This article has been cited 28 times.- Phyo H, Aburza A, Mellanby K, Esteves CL. Characterization of canine adipose- and endometrium-derived Mesenchymal Stem/Stromal Cells and response to lipopolysaccharide.. Front Vet Sci 2023;10:1180760.
- Kim YS, Oh SM, Suh DS, Tak DH, Kwon YB, Koh YG. Cartilage lesion size and number of stromal vascular fraction (SVF) cells strongly influenced the SVF implantation outcomes in patients with knee osteoarthritis.. J Exp Orthop 2023 Mar 15;10(1):28.
- Caruso M, Shuttle S, Amelse L, Elkhenany H, Schumacher J, Dhar MS. A pilot study to demonstrate the paracrine effect of equine, adult allogenic mesenchymal stem cells in vitro, with a potential for healing of experimentally-created, equine thoracic wounds in vivo.. Front Vet Sci 2022;9:1011905.
- Chen R, Jiang Y, Lu L, Wang P, Huang D, Wang J, Liu Z, Qin S, Yin F. Bibliometric analysis of research trends in stem cell therapy for knee osteoarthritis over the period 2001-2021.. Front Cell Dev Biol 2022;10:996273.
- Onoi Y, Hiranaka T, Hida Y, Fujishiro T, Okamoto K, Matsumoto T, Kuroda R. Second-Look Arthroscopic Findings and Clinical Outcomes after Adipose-Derived Regenerative Cell Injection in Knee Osteoarthritis.. Clin Orthop Surg 2022 Sep;14(3):377-385.
- Yue D, Du L, Zhang B, Wu H, Yang Q, Wang M, Pan J. Time-dependently Appeared Microenvironmental Changes and Mechanism after Cartilage or Joint Damage and the Influences on Cartilage Regeneration.. Organogenesis 2021 Oct 2;17(3-4):85-99.
- Lee S, Chae DS, Song BW, Lim S, Kim SW, Kim IK, Hwang KC. ADSC-Based Cell Therapies for Musculoskeletal Disorders: A Review of Recent Clinical Trials.. Int J Mol Sci 2021 Sep 30;22(19).
- Zheng W, Li H, Hu K, Li L, Bei M. Chondromalacia patellae: current options and emerging cell therapies.. Stem Cell Res Ther 2021 Jul 18;12(1):412.
- Bertoni L, Jacquet-Guibon S, Branly T, Desancu00e9 M, Legendre F, Melin M, Rivory P, Hartmann DJ, Schmutz A, Denoix JM, Demoor M, Audigiu00e9 F, Galu00e9ra P. Evaluation of Allogeneic Bone-Marrow-Derived and Umbilical Cord Blood-Derived Mesenchymal Stem Cells to Prevent the Development of Osteoarthritis in An Equine Model.. Int J Mol Sci 2021 Mar 2;22(5).
- Shahsavari A, Weeratunga P, Ovchinnikov DA, Whitworth DJ. Pluripotency and immunomodulatory signatures of canine induced pluripotent stem cell-derived mesenchymal stromal cells are similar to harvested mesenchymal stromal cells.. Sci Rep 2021 Feb 10;11(1):3486.
- Kim YS, Suh DS, Tak DH, Chung PK, Koh YG. Mesenchymal Stem Cell Implantation in Knee Osteoarthritis: Midterm Outcomes and Survival Analysis in 467 Patients.. Orthop J Sports Med 2020 Dec;8(12):2325967120969189.
- Groen WMGAC, Utomo L, Castilho M, Gawlitta D, Malda J, Weeren PRV, Levato R, Korthagen NM. Impact of Endotoxins in Gelatine Hydrogels on Chondrogenic Differentiation and Inflammatory Cytokine Secretion In Vitro.. Int J Mol Sci 2020 Nov 13;21(22).
- Kim YS, Suh DS, Tak DH, Chung PK, Kwon YB, Kim TY, Koh YG. Comparative matched-pair cohort analysis of the short-term clinical outcomes of mesenchymal stem cells versus hyaluronic acid treatments through intra-articular injections for knee osteoarthritis.. J Exp Orthop 2020 Nov 13;7(1):90.
- Cifu00f9 A, Domenis R, Pozzi-Mucelli M, Di Benedetto P, Causero A, Moretti M, Stevanato M, Pistis C, Parodi PC, Fabris M, Curcio F. The Exposure to Osteoarthritic Synovial Fluid Enhances the Immunomodulatory Profile of Adipose Mesenchymal Stem Cell Secretome.. Stem Cells Int 2020;2020:4058760.
- Bundgaard L, Stensballe A, Elbu00e6k KJ, Berg LC. Mass spectrometric analysis of the in vitro secretome from equine bone marrow-derived mesenchymal stromal cells to assess the effect of chondrogenic differentiation on response to interleukin-1u03b2 treatment.. Stem Cell Res Ther 2020 May 20;11(1):187.
- Kim GB, Shon OJ. Current perspectives in stem cell therapies for osteoarthritis of the knee.. Yeungnam Univ J Med 2020 Jul;37(3):149-158.
- Li J, Shao Q, Zhu X, Sun G. Efficacy of autologous bone marrow mesenchymal stem cells in the treatment of knee osteoarthritis and their effects on the expression of serum TNF-u03b1 and IL-6.. J Musculoskelet Neuronal Interact 2020 Mar 3;20(1):128-135.
- Colbath AC, Dow SW, Hopkins LS, Phillips JN, McIlwraith CW, Goodrich LR. Single and repeated intra-articular injections in the tarsocrural joint with allogeneic and autologous equine bone marrow-derived mesenchymal stem cells are safe, but did not reduce acute inflammation in an experimental interleukin-1u03b2 model of synovitis.. Equine Vet J 2020 Jul;52(4):601-612.
- Bertoni L, Branly T, Jacquet S, Desancu00e9 M, Desquilbet L, Rivory P, Hartmann DJ, Denoix JM, Audigiu00e9 F, Galu00e9ra P, Demoor M. Intra-Articular Injection of 2 Different Dosages of Autologous and Allogeneic Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stem Cells Triggers a Variable Inflammatory Response of the Fetlock Joint on 12 Sound Experimental Horses.. Stem Cells Int 2019;2019:9431894.
- Lee WS, Kim HJ, Kim KI, Kim GB, Jin W. Intra-Articular Injection of Autologous Adipose Tissue-Derived Mesenchymal Stem Cells for the Treatment of Knee Osteoarthritis: A Phase IIb, Randomized, Placebo-Controlled Clinical Trial.. Stem Cells Transl Med 2019 Jun;8(6):504-511.
- Hill ABT, Bressan FF, Murphy BD, Garcia JM. Applications of mesenchymal stem cell technology in bovine species.. Stem Cell Res Ther 2019 Jan 24;10(1):44.
- Broeckx SY, Seys B, Suls M, Vandenberghe A, Mariu00ebn T, Adriaensen E, Declercq J, Van Hecke L, Braun G, Hellmann K, Spaas JH. Equine Allogeneic Chondrogenic Induced Mesenchymal Stem Cells Are an Effective Treatment for Degenerative Joint Disease in Horses.. Stem Cells Dev 2019 Mar 15;28(6):410-422.
- Cortu00e9s-Araya Y, Amilon K, Rink BE, Black G, Lisowski Z, Donadeu FX, Esteves CL. Comparison of Antibacterial and Immunological Properties of Mesenchymal Stem/Stromal Cells from Equine Bone Marrow, Endometrium, and Adipose Tissue.. Stem Cells Dev 2018 Nov 1;27(21):1518-1525.
- Yang Z, Concannon J, Ng KS, Seyb K, Mortensen LJ, Ranganath S, Gu F, Levy O, Tong Z, Martyn K, Zhao W, Lin CP, Glicksman MA, Karp JM. Tetrandrine identified in a small molecule screen to activate mesenchymal stem cells for enhanced immunomodulation.. Sci Rep 2016 Jul 26;6:30263.
- Carmona JU, Ru00edos DL, Lu00f3pez C, u00c1lvarez ME, Pu00e9rez JE, Bohu00f3rquez ME. In vitro effects of platelet-rich gel supernatants on histology and chondrocyte apoptosis scores, hyaluronan release and gene expression of equine cartilage explants challenged with lipopolysaccharide.. BMC Vet Res 2016 Jul 1;12(1):135.
- Barrachina L, Remacha AR, Romero A, Vu00e1zquez FJ, Albareda J, Prades M, Ranera B, Zaragoza P, Martu00edn-Burriel I, Rodellar C. Inflammation affects the viability and plasticity of equine mesenchymal stem cells: possible implications in intra-articular treatments.. J Vet Sci 2017 Mar 30;18(1):39-49.
- Lam J, Lu S, Lee EJ, Trachtenberg JE, Meretoja VV, Dahlin RL, van den Beucken JJ, Tabata Y, Wong ME, Jansen JA, Mikos AG, Kasper FK. Osteochondral defect repair using bilayered hydrogels encapsulating both chondrogenically and osteogenically pre-differentiated mesenchymal stem cells in a rabbit model.. Osteoarthritis Cartilage 2014 Sep;22(9):1291-300.
- Wu L, Liu H, Zhang R, Li L, Li J, Hu H, Huang H. Chondroprotective Activity of Murraya exotica through Inhibiting u03b2 -Catenin Signaling Pathway.. Evid Based Complement Alternat Med 2013;2013:752150.
Secure Payments
