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Veterinary surgery : VS2021; 50(5); 1117-1127; doi: 10.1111/vsu.13608

Influence of a novel scaffold composed of polyurethane, hydroxyapatite, and decellularized bone particles on the healing of fourth metacarpal defects in mares.

Abstract: To determine the effect of a novel scaffold, designed for use in bone regeneration, on healing of splint bone segmental defects in mares. Methods: In vivo experimental study. Methods: Five adult mares (4-10 years old; mean weight, 437.7 kg ± 29 kg). Methods: Bilateral 2-cm full-thickness defects were created in the fourth metacarpal bones (MCIV) of each horse. Each defect was randomly assigned to either a novel scaffold treatment (n = 5) or an untreated control (n = 5). The scaffold was composed of polyurethane, hydroxyapatite, and decellularized bone particles. Bone healing was assessed for a period of 60 days by thermography, ultrasonography, radiography, and computed tomography (CT). Biopsies of each defect were performed 60 days after surgery for histological evaluation. Results: On the basis of radiographic analysis, scaffold-treated defects had greater filling (67.42% ± 26.7%) compared with untreated defects (35.88% ± 32.7%; P = .006). After 60 days, CT revealed that the density of the defects treated with the scaffolds (807.80 ± 129.6 Hounsfield units [HU]) was greater than density of the untreated defects (464.80 ± 81.3 HU; P = .004). Evaluation of histology slides provided evidence of bone formation within an average of 9.43% ± 3.7% of the cross-sectional area of scaffolds in contrast to unfilled defects in which connective tissue was predominant throughout the biopsy specimens. Conclusions: The novel scaffold was biocompatible and supported bone formation within the MCIV segmental defects. Conclusions: This novel scaffold offers an effective option for filling bone voids in horses when support of bone healing is indicated.
© 2021 The Authors. Veterinary Surgery published by Wiley Periodicals LLC on behalf of American College of Veterinary Surgeons.
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Publication Date: 2021-05-05 PubMed ID: 33948951PubMed Central: PMC8360067DOI: 10.1111/vsu.13608Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study aimed to examine the impact of a new scaffold, designed to aid bone regeneration, on the healing of splint bone segmental defects in mares. The results indicated that the new scaffold was biocompatible and assisted in bone formation within the segmental defects.

Study Design and Methodology

  • The research was an in vivo experimental study conducted on five adult mares aged between four and ten. These mares had a mean weight of 437.7 kg.
  • The researchers created bilateral full-thickness defects, measuring 2 cm, in the mares’ fourth metacarpal bones. These defects were randomly designated to be either scaffold treatment or untreated control.
  • The novel scaffold utilised in the study was made from polyurethane, hydroxyapatite and decellularized bone particles. Bone healing was evaluated for sixty days with the aid of thermography, radiography, ultrasonography, and computed tomography (CT).
  • Biopsies of each defect were conducted sixty days after the surgery to aid histological examination.

Outcome and Results

  • Based on radiographic analysis, defects treated with the scaffold demonstrated greater filling (67.42%) in comparison with the untreated ones (35.88%).
  • Post sixty days, CT scans revealed that the defects treated with the scaffold displayed greater density than the density observed in untreated defects.
  • Histological examination of the slides showed evidence of bone formation within an average area of 9.43% of the scaffold. In contrast, untreated defects predominantly contained connective tissue throughout the biopsy samples.

Conclusions

  • The study concluded that the new scaffold was biocompatible and facilitated bone formation within the segmental defects of the metacarpal bones in mares.
  • The findings suggest that this novel scaffold may serve as an effective solution for filling bone voids in horses when there is an indicated need for bone healing support.

Cite This Article

APA
Grzeskowiak RM, Alghazali KM, Hecht S, Donnell RL, Doherty TJ, Smith CK, Anderson DE, Biris AS, Adair HS. (2021). Influence of a novel scaffold composed of polyurethane, hydroxyapatite, and decellularized bone particles on the healing of fourth metacarpal defects in mares. Vet Surg, 50(5), 1117-1127. https://doi.org/10.1111/vsu.13608

Publication

Veterinary surgery : VS
ISSN: 1532-950X
NlmUniqueID: 8113214
Country: United States
Language: English
Volume: 50
Issue: 5
Pages: 1117-1127

Researcher Affiliations

Grzeskowiak, Remigiusz M
  • Department of Large Animal Clinical Sciences, The University of Tennessee College of Veterinary Medicine, Knoxville, Tennessee, USA.
Alghazali, Karrer M
  • Center for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, Little Rock, Arkansas, USA.
Hecht, Silke
  • Department of Small Animal Clinical Sciences, The University of Tennessee College of Veterinary Medicine, Knoxville, Tennessee, USA.
Donnell, Robert L
  • Department of Biomedical and Diagnostic Sciences, The University of Tennessee College of Veterinary Medicine, Knoxville, Tennessee, USA.
Doherty, Thomas J
  • Department of Large Animal Clinical Sciences, The University of Tennessee College of Veterinary Medicine, Knoxville, Tennessee, USA.
Smith, Christopher K
  • Department of Small Animal Clinical Sciences, The University of Tennessee College of Veterinary Medicine, Knoxville, Tennessee, USA.
Anderson, David E
  • Department of Large Animal Clinical Sciences, The University of Tennessee College of Veterinary Medicine, Knoxville, Tennessee, USA.
Biris, Alexandru S
  • Center for Integrative Nanotechnology Sciences, University of Arkansas at Little Rock, Little Rock, Arkansas, USA.
Adair, Henry S
  • Department of Large Animal Clinical Sciences, The University of Tennessee College of Veterinary Medicine, Knoxville, Tennessee, USA.

MeSH Terms

  • Animals
  • Biocompatible Materials
  • Bone Regeneration
  • Bone and Bones
  • Durapatite
  • Female
  • Guided Tissue Regeneration / veterinary
  • Horse Diseases / surgery
  • Horses
  • Metacarpal Bones / diagnostic imaging
  • Metacarpal Bones / injuries
  • Metacarpal Bones / pathology
  • Polyurethanes
  • Tissue Scaffolds / veterinary
  • Wound Healing

Grant Funding

  • Karen Gilhousen Equine Bone Regeneration Fund, Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee.

Conflict of Interest Statement

D.E. Anderson and A. S. Biris A.S. have co‐ownership in the regenerative novel scaffold technology used in this research that has been licensed by the University of Arkansas at Little Rock to NuShores Biosciences LLC. All other authors declare no conflicts of interest related to this report.

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Citations

This article has been cited 2 times.
  1. Schulze F, Lang A, Schoon J, Wassilew GI, Reichert J. Scaffold Guided Bone Regeneration for the Treatment of Large Segmental Defects in Long Bones. Biomedicines 2023 Jan 24;11(2).
    doi: 10.3390/biomedicines11020325pubmed: 36830862google scholar: lookup
  2. Yazdanian A, Jahandideh A, Hesaraki S. The effect of green synthesis of TiO(2) nanoparticles/collagen/HA scaffold in bone regeneration: As an animal study. Vet Med Sci 2023 Sep;9(5):2342-2351.
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