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Home / Equine Research Bank / Theriogenology / Influence of bacteria and gentamicin on cooled-stored stalli...
Theriogenology2006; 67(5); 912-918; doi: 10.1016/j.theriogenology.2006.11.004

Influence of bacteria and gentamicin on cooled-stored stallion spermatozoa.

Abstract: This study investigated effects of bacteria from the genital tract of horses and the effect of gentamicin in semen extender on spermatozoal function in cooled-stored stallion semen. Semen was collected from healthy stallions and processed with a milk-based extender with or without gentamicin (1g/l). Pseudomonas (Ps.) aeruginosa, Staphylococcus (St.) aureus, Streptococcus (Sc.) equi subsp. equi (Sc. equi), Sc. equi subsp. zooepidemicus (Sc. zooepidemicus), Sc. dysgalactiae subsp. equisimilis (Sc. equisimilis) or culture medium alone (control) were added. Immediately after addition of bacteria and after storage at 5 degrees C for 24, 48 and 72h, motility, velocity and membrane integrity of diluted semen were determined with a CASA system. After 24h, semen with Ps. aeruginosa and Sc. equisimilis showed significantly lower motility and velocity compared to all other groups; after 72h these differences still existed for Ps. aeruginosa (p<0.05). The percentage of membrane-intact spermatozoa was significantly lower after 24h of storage in spermatozoa incubated with Sc. equisimilis and after 72h with Sc. equisimilis and Ps. aeruginosa. Addition of gentamicin to extender resulted in decreased motility and velocity in semen without addition of bacteria and did not improve motility parameters in semen with bacteria added. In conclusion, certain bacteria may have detrimental effects on semen quality during cooled-storage. These effects are not reduced by addition of gentamicin. Gentamicin can negatively affect spermatozoal function in extended semen during cooled-storage and therefore, optimal concentrations have to be tested for the respective extender medium.
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Publication Date: 2006-12-01 PubMed ID: 17141306DOI: 10.1016/j.theriogenology.2006.11.004Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about a study on how bacteria from the genital tract of horses, and the addition of gentamicin to semen extender, affect the function of spermatozoa in cooled-stored stallion semen.

Research Objective and Methodology

  • The goal of this research was to understand how the presence of different bacteria in the genital tract of horses and the use of gentamicin (an antibiotic) in semen extenders impact the quality of cooled-stored stallion semen.
  • Semen was collected from healthy stallions and processed either with a milk-based extender with or without the addition of 1g/l of gentamicin.
  • The researchers then added different types of bacteria to the semen, including Pseudomonas aeruginosa, Staphylococcus aureus, varieties of Streptococcus, or a control culture medium with no additional bacteria.
  • The researchers assessed the motility, velocity, and membrane integrity of the semen immediately after the addition of bacteria and after storage at 5 degrees C for 24, 48, and 72 hours using a Computer-Assisted Sperm Analysis (CASA) system.

Main Findings

  • After 24 hours, the researchers found that semen samples that had been exposed to Pseudomonas aeruginosa and Streptococcus dysgalactiae subsp. equisimilis exhibited significantly lower motility and velocity compared to other groups.
  • 72 hours after the introduction of bacteria, these differences in motility and velocity persisted for samples that had been contaminated with Pseudomonas aeruginosa.
  • The proportion of membrane-intact spermatozoa was notably lower after 24 hours of storage in semen samples which had been treated with Streptococcus dysgalactiae subsp. equisimilis, and after 72 hours in those that had been treated with Streptococcus dysgalactiae subsp. equisimilis and Pseudomonas aeruginosa.
  • The use of gentamicin in the semen extender led to a decrease in motility and velocity in semen where no bacteria had been added, and did not improve these parameters in semen where bacteria had been introduced.

Conclusions

  • The study concluded that certain types of bacteria can have a harmful effect on the quality of cooled-stored stallion semen.
  • These negative effects were not mitigated by the addition of gentamicin to the semen extender. In fact, gentamicin resulted in a negative impact on the function of the spermatozoa in extended semen during cooled storage.
  • The study suggests that the optimal concentrations of gentamicin need to be tested for each specific semen extender medium.

Cite This Article

APA
Aurich C, Spergser J. (2006). Influence of bacteria and gentamicin on cooled-stored stallion spermatozoa. Theriogenology, 67(5), 912-918. https://doi.org/10.1016/j.theriogenology.2006.11.004

Publication

Theriogenology
ISSN: 0093-691X
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 67
Issue: 5
Pages: 912-918

Researcher Affiliations

Aurich, C
  • Centre for Artificial Insemination and Embryo Transfer, Department of Animal Breeding and Reproduction, University for Veterinary Sciences, Veterinärplatz 1, 1210 Vienna, Austria. christine.aurich@vu-wien.ac.at
Spergser, J

    MeSH Terms

    • Animals
    • Anti-Bacterial Agents / pharmacology
    • Bacteria / growth & development
    • Bacterial Infections / microbiology
    • Bacterial Infections / prevention & control
    • Bacterial Infections / veterinary
    • Female
    • Gentamicins / pharmacology
    • Horses / microbiology
    • Male
    • Semen Preservation / methods
    • Semen Preservation / veterinary
    • Sperm Count / veterinary
    • Sperm Motility / drug effects
    • Sperm Motility / physiology
    • Spermatozoa / microbiology

    Citations

    This article has been cited 19 times.
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