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Home / Equine Research Bank / Acta Histochem / Influence of mechanical fluid shear stress on the osteogenic...
Acta histochemica2019; 121(3); 344-353; doi: 10.1016/j.acthis.2019.02.002

Influence of mechanical fluid shear stress on the osteogenic differentiation protocols for Equine adipose tissue-derived mesenchymal stem cells.

Abstract: Cell-based therapies have become a promising approach to promote tissue regeneration and the treatment of musculoskeletal disorders. Bone regeneration maintains bone homeostasis, mechanical stability and physical performance. Mechanical stimulation showed to induce stem cell differentiation into the osteogenic fate. However, the effect of various osteogenic protocols on the osteogenic commitment of equine adipose-derived stem cells is not fully elucidated. Here we examined the influence of fluid-based shear stress (FSS) via mechanical rocking to assess whether mechanical stimulation promotes osteogenic differentiation of equine adipose-derived stem cells (ASCs). ASCs were induced into osteogenic fate using osteogenic differentiation medium (ODM) protocol or additional supplementation of 5 mM CaCl and 7.5 mM CaCl protocol compared to cells cultivated in basal medium (BM) up to 21 day. The ASCs proliferation pattern was evaluated using the sulforhodamine B (SRB) protein assay. Osteogenic differentiation examined via semi-quantification of alizarin red staining (ARS) and alkaline phosphatase activity (ALP) as well as, via quantification of osteocalcin (OC), alkaline phosphatase (ALP), osteopontin (OP), and collagen type-1 (COL1) gene expression using RT-qPCR. We show that mechanical FSS increased the proliferation pattern of ASCs compared to the static conditions. Mechanical FSS together with 5 mM CaCl and 7.5 mM CaCl promoted osteogenic nodule formation and increased ARS intensity compared to the standard osteogenic protocols. We observed that combined mechanical FSS with ODM protocol increase ALP activity compared to static culture conditions. We report that ALP and OC osteogenic markers expression were upregulated under mechanical FSS culture condition particularly with the ODM protocol. Taken together, it can be assumed that mechanical stress using FSS promotes the efficiency of the osteogenic differentiation protocols of ASCs through independent mechanisms.
Copyright © 2019 Elsevier GmbH. All rights reserved.
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Publication Date: 2019-02-23 PubMed ID: 30808518DOI: 10.1016/j.acthis.2019.02.002Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper focuses on the impact of mechanical fluid shear stress (FSS) on the osteogenic differentiation protocols for equine adipose tissue-derived mesenchymal stem cells (ASCs). The study finds that utilizing mechanical forces such as FSS can increase the efficacy and efficiency of stem cell differentiation to osteogenic fate.

Explanation of Bone Regeneration

  • Bone regeneration is essential for maintaining bone homeostasis, mechanical stability, and physical performance. It’s a common treatment approach for various musculoskeletal disorders.
  • Cell-based therapies, including those utilizing mesenchymal stem cells, are a common approach to promote tissue regeneration, including bone regeneration.

Role of Mechanical Stimulation and Fluid Shear Stress (FSS)

  • Mechanical stimulations like FSS can induce stem cell differentiation into osteoblasts – cells responsible for bone formation.
  • This research particularly focuses on the application of FSS on the equine adipose-derived stem cells (ASCs) to promote their osteogenic differentiation.

Experimental Design and Results

  • The research used various osteogenic protocols, like osteogenic differentiation medium (ODM) and supplementation with Calcium Chloride (5 mM CaCl, 7.5 mM CaCl).
  • The proliferation pattern of ASCs was evaluated through the sulforhodamine B (SRB) protein assay. The osteogenic differentiation was assessed through semi-quantification of alizarin red staining (ARS), alkaline phosphatase activity (ALP), and quantification of specific gene expressions.
  • The application of mechanical FSS increased the proliferation rate of ASCs compared to the static conditions. Also, FSS in combination with Calcium Chloride promoted the formation of osteogenic nodules and intensified ARS compared to the standard osteogenic protocols.
  • The combined mechanical FSS with ODM protocol led to increased ALP activity compared to static culture conditions.
  • Osteogenic markers like ALP and osteocalcin (OC) were upregulated when subjected to mechanical FSS culture condition, particularly with the ODM protocol.

Conclusions

  • The research concludes that mechanical stress using FSS enhances the efficiency of osteogenic differentiation protocols of ASCs, suggesting new potential strategies for optimizing bone regeneration.

Cite This Article

APA
Elashry MI, Gegnaw ST, Klymiuk MC, Wenisch S, Arnhold S. (2019). Influence of mechanical fluid shear stress on the osteogenic differentiation protocols for Equine adipose tissue-derived mesenchymal stem cells. Acta Histochem, 121(3), 344-353. https://doi.org/10.1016/j.acthis.2019.02.002

Publication

Acta histochemica
ISSN: 1618-0372
NlmUniqueID: 0370320
Country: Germany
Language: English
Volume: 121
Issue: 3
Pages: 344-353
PII: S0065-1281(18)30122-3

Researcher Affiliations

Elashry, Mohamed I
  • Institute of Veterinary Anatomy-, Histology and -Embryology, University of Giessen, 35392, Giessen, Germany; Anatomy and Embryology Department, Faculty of Veterinary Medicine, University of Mansoura, 35516, Egypt. Electronic address: mohammed.elashry@vetmed.uni-giessen.de.
Gegnaw, Shumet T
  • Institute of Veterinary Anatomy-, Histology and -Embryology, University of Giessen, 35392, Giessen, Germany; Institute des Neurosciences Cellulaires et Integratives (INCI), University of Strasbourg, 67084, Strasbourg, France.
Klymiuk, Michele C
  • Institute of Veterinary Anatomy-, Histology and -Embryology, University of Giessen, 35392, Giessen, Germany.
Wenisch, Sabine
  • Clinic of Small Animals, c/o Institute of Veterinary Anatomy, Histology and Embryology, Justus Liebig University of Giessen, 35392, Germany.
Arnhold, Stefan
  • Institute of Veterinary Anatomy-, Histology and -Embryology, University of Giessen, 35392, Giessen, Germany.

MeSH Terms

  • Adipose Tissue / cytology
  • Alkaline Phosphatase / metabolism
  • Animals
  • Bone and Bones / metabolism
  • Cell Differentiation / physiology
  • Cell Proliferation / physiology
  • Cells, Cultured
  • Collagen Type I / metabolism
  • Horses
  • Mesenchymal Stem Cells / cytology
  • Osteocalcin / genetics
  • Osteogenesis / physiology

Citations

This article has been cited 13 times.
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