Inhibitory effect of curcuminoids and tetrahydrocurcuminoids on equine activated neutrophils and myeloperoxidase activity.
Abstract: In the horse, the inflammation response to various pathologies (intestinal strangulations, laminitis, etc.) involves an excessive stimulation of the polymorphonuclear neutrophils releasing reactive oxygen species (ROS) and myeloperoxidase (MPO). The aim of the present work was to study the effect of natural polyphenols, curcuminoids and tetrahydrocurcuminoids (THC) on isolated stimulated equine neutrophils and on the activity of purified MPO. The ROS production and the release of MPO by activated neutrophils were measured by chemiluminescence and ELISA techniques, respectively. The activity of purified MPO was measured by studying its nitration, chlorination or oxidation capacity and by using an original method called SIEFED allowing the study of drug interaction with the enzyme without interferences of the medium. Curcuminoids and THC had dose-dependent inhibitory effects on ROS production and MPO release by activated neutrophils and on purified MPO activity. We suggest that the higher efficacy of curcuminoids versus THC could be explained, at least partially, by its chemical structure: the conjugated double bounds and the plane structure of curcuminoids made easier the neutralization of the radical species generated by activated neutrophils and the interaction of the drug with the active site of MPO. These inhibitory effects of curcuminoids on the oxidant activity of equine neutrophils and on MPO activity open therapeutic perspectives in equine pathologies with excessive inflammatory reactions.
Publication Date: 2007-07-26 PubMed ID: 17705665DOI: 10.33549/physiolres.931086Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research paper discusses the effects of curcuminoids and tetrahydrocurcuminoids (THC) on inflammation reactions in horses. The study found that these compounds can reduce the production of harmful substances by neutrophils – a type of white blood cell – which could potentially help to treat equine diseases associated with excessive inflammation.
Research Objective
- This research was aiming to investigate the potential anti-inflammatory benefits of curcuminoids and tetrahydrocurcuminoids (THC) on horses. These compounds are natural polyphenols with reputed health benefits, and the researchers sought to examine their effect on neutrophils, a type of white blood cell, and the enzyme myeloperoxidase (MPO), which is also involved in inflammation responses.
Methodology
- The researchers stimulated isolated equine neutrophils and measured the neutrophils’ release of reactive oxygen species (ROS) and MPO, using chemiluminescence and an Enzyme-Linked Immunosorbent Assay (ELISA) respectively.
- The activity of purified MPO was then measured under three conditions – nitration, chlorination and oxidation – using a method called SIEFED, which allows for the study of drug interaction with the enzyme without interference from the surrounding medium.
Findings
- The study found that both curcuminoids and THC had a dose-dependent inhibitory effect on both ROS production and MPO release by activated neutrophils. They also inhibited the activity of purified MPO.
- Interestingly, the efficacy of curcuminoids was higher than that of THC, a difference which the researchers attribute to the chemical structure of curcuminoids, namely their conjugated double bound and plane structures which are likely to facilitate neutralization of radical species generated by activated neutrophils and interaction with the active site of MPO.
Conclusion
- The research concludes that the inhibitory effects of curcuminoids on the oxidant activity of equine neutrophils and on MPO activity hint at potential therapeutic applications in treating equine diseases caused by excessive inflammatory responses.
Cite This Article
APA
Franck T, Kohnen S, Grulke S, Neven P, Goutman Y, Peters F, Pirotte B, Deby-Dupont G, Serteyn D.
(2007).
Inhibitory effect of curcuminoids and tetrahydrocurcuminoids on equine activated neutrophils and myeloperoxidase activity.
Physiol Res, 57(4), 577-587.
https://doi.org/10.33549/physiolres.931086 Publication
Researcher Affiliations
- Department of Clinical Sciences, Equine Clinic, Veterinary Institute B 41, University of Liège - Sart Tilman, Liège, Belgium. t.franck@ulg.ac.be
MeSH Terms
- Animals
- Benzothiazoles / metabolism
- Cell Separation
- Cell Survival / drug effects
- Chlorides / metabolism
- Curcumin / analogs & derivatives
- Curcumin / pharmacology
- Flavonoids / chemistry
- Flavonoids / pharmacology
- Horses / physiology
- Immunohistochemistry
- In Vitro Techniques
- Luminescence
- Neutrophil Activation / drug effects
- Nitrates / metabolism
- Oxidation-Reduction
- Peroxidase / metabolism
- Phenols / chemistry
- Phenols / pharmacology
- Polyphenols
- Reactive Oxygen Species / metabolism
- Sulfonic Acids / metabolism
- Taurine / metabolism
- Tyrosine / pharmacology
Citations
This article has been cited 9 times.- Franck T, Ceusters J, Graide H, Niesten A, Duysens J, Mickalad AM, Serteyn D. Equine Muscle Derived Mesenchymal Stem Cells Loaded with Water-Soluble Curcumin: Modulation of Neutrophil Activation and Enhanced Protection against Intracellular Oxidative Attack. Int J Mol Sci 2023 Jan 5;24(2).
- Bulfon C, Galeotti M, Volpatti D. Medicinal plant extracts modulate respiratory burst and proliferation activity of rainbow trout (Oncorhynchus mykiss) leukocytes. Fish Physiol Biochem 2018 Feb;44(1):109-117.
- Arshad L, Jantan I, Bukhari SN, Haque MA. Immunosuppressive Effects of Natural α,β-Unsaturated Carbonyl-Based Compounds, and Their Analogs and Derivatives, on Immune Cells: A Review. Front Pharmacol 2017;8:22.
- Kato Y. Neutrophil myeloperoxidase and its substrates: formation of specific markers and reactive compounds during inflammation. J Clin Biochem Nutr 2016 Mar;58(2):99-104.
- Derochette S, Mouithys-Mickalad A, Franck T, Collienne S, Ceusters J, Deby-Dupont G, Neven P, Serteyn D. NDS27 combines the effect of curcumin lysinate and hydroxypropyl-β-cyclodextrin to inhibit equine PKCδ and NADPH oxidase involved in the oxidative burst of neutrophils. FEBS Open Bio 2014;4:1021-9.
- Drábiková K, Perečko T, Nosáľ R, Harmatha J, Smidrkal J, Jančinová V. Polyphenol derivatives - potential regulators of neutrophil activity. Interdiscip Toxicol 2012 Jun;5(2):65-70.
- Tsumbu CN, Deby-Dupont G, Tits M, Angenot L, Frederich M, Kohnen S, Mouithys-Mickalad A, Serteyn D, Franck T. Polyphenol content and modulatory activities of some tropical dietary plant extracts on the oxidant activities of neutrophils and myeloperoxidase. Int J Mol Sci 2012;13(1):628-650.
- Liu YJ, Ye QW, Jin YL, Zhang Q, Zou X, Li JP. Spatial Domain-Based Approach to Analyze the Mechanism of Sparganii Rhizoma-Curcumae Rhizoma Pair in the Treatment of Colorectal Cancer. Food Sci Nutr 2025 Aug;13(8):e70794.
- Abul N, Demir Y, Öztekin A, Özdemir H. Determination of the Inhibitory Potential of Chalcones on Myeloperoxidase Enzyme Activity: In vitro and Molecular Docking Studies. Cell Biochem Biophys 2025 Sep;83(3):3383-3393.
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