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Scientific reports2016; 6; 22932; doi: 10.1038/srep22932

Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse.

Abstract: The adaptive response to extreme endurance exercise might involve transcriptional and translational regulation by microRNAs (miRNAs). Therefore, the objective of the present study was to perform an integrated analysis of the blood transcriptome and miRNome (using microarrays) in the horse before and after a 160 km endurance competition. A total of 2,453 differentially expressed genes and 167 differentially expressed microRNAs were identified when comparing pre- and post-ride samples. We used a hypergeometric test and its generalization to gain a better understanding of the biological functions regulated by the differentially expressed microRNA. In particular, 44 differentially expressed microRNAs putatively regulated a total of 351 depleted differentially expressed genes involved variously in glucose metabolism, fatty acid oxidation, mitochondrion biogenesis, and immune response pathways. In an independent validation set of animals, graphical Gaussian models confirmed that miR-21-5p, miR-181b-5p and miR-505-5p are candidate regulatory molecules for the adaptation to endurance exercise in the horse. To the best of our knowledge, the present study is the first to provide a comprehensive, integrated overview of the microRNA-mRNA co-regulation networks that may have a key role in controlling post-transcriptomic regulation during endurance exercise.
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Publication Date: 2016-03-10 PubMed ID: 26960911PubMed Central: PMC4785432DOI: 10.1038/srep22932Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study has analyzed changes in gene and microRNA expression that are potentially associated with adaptation to endurance exercise in horses, and identified several specific microRNAs that regulate resistance to intense physical activity.

Objective of the Research

  • In this study, the researchers aimed to investigate the way extreme endurance exercise in horses affects their gene and microRNA (small RNA molecules that help regulate gene expression) profiles.
  • They conducted a comprehensive analysis of both the transcriptome (all RNA molecules) and miRNome (all microRNAs) of the blood of horses before and after a 160km endurance competition.

Methodology and Findings

  • They identified 2,453 differentially expressed genes and 167 differentially expressed microRNAs between the pre- and post- competition samples.
  • The researchers performed a hypergeometric test and its generalization to better understand the biological functions influenced by these differentially expressed microRNAs.
  • 44 differentially expressed microRNAs were found to regulate 351 genes in processes including glucose metabolism, fatty acid oxidation, mitochondria creation (crucial for energy production), and immune response pathways.

Validation and Conclusion

  • The findings were then validated in an independent set of horses using Graphical Gaussian models.
  • Three miRNAs (miR-21-5p, miR-181b-5p and miR-505-5p) were singled out as candidate regulatory molecules that help horses adapt to endure physical exercise.
  • The researchers state that this is the first study to comprehensively show the co-regulation networks of microRNAs and mRNA potentially essential for post-transcriptomic regulation during endurance exercise. This understanding could have long-term implications in the field of sportsmedicine and animal physiology.

Cite This Article

APA
Mach N, Plancade S, Pacholewska A, Lecardonnel J, Rivière J, Moroldo M, Vaiman A, Morgenthaler C, Beinat M, Nevot A, Robert C, Barrey E. (2016). Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse. Sci Rep, 6, 22932. https://doi.org/10.1038/srep22932

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 6
Pages: 22932

Researcher Affiliations

Mach, Núria
  • Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
Plancade, Sandra
  • INRA, MaIAGE, Jouy-en-Josas, France.
Pacholewska, Alicja
  • Swiss Institute of Equine Medicine, Institute of Genetics, University of Bern and Agroscope, Bern, Switzerland.
Lecardonnel, Jérôme
  • Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
Rivière, Julie
  • Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
Moroldo, Marco
  • Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
Vaiman, Anne
  • Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
Morgenthaler, Caroline
  • Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
Beinat, Marine
  • Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
Nevot, Alizée
  • Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
Robert, Céline
  • Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
  • Université Paris-Est, Ecole Vétérinaire d'Alfort, Maisons-Alfort, France.
Barrey, Eric
  • Animal Genetics and Integrative Biology unit (GABI), INRA, AgroParis Tech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
  • Integrative Biology and Exercice Adaptation unit (UBIAE), EA7362, Université d'Evry Val d'Essonne, Evry, France.

MeSH Terms

  • Animals
  • Biomarkers / metabolism
  • Gene Expression Regulation
  • Horses / genetics
  • Horses / physiology
  • MicroRNAs / genetics
  • MicroRNAs / isolation & purification
  • Physical Endurance / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification

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