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Home / Equine Research Bank / Arch Virol / Inter- and intra-strain genomic variation in equine herpesvi...
Archives of virology1994; 134(1-2); 169-178; doi: 10.1007/BF01379115

Inter- and intra-strain genomic variation in equine herpesvirus type 1 isolates.

Abstract: Restriction enzyme digests of DNA from 22 unselected isolates of EHV-1 were analysed by hybridization with cloned DNA fragments covering the genome. In addition to a small amount of inter-strain variation, heterogeneity within strains was observed, caused by loss of specific restriction endonuclease sites in the DNA of a proportion of the virus particles of any one stock. Fifteen strains demonstrated the same intra-strain variation involving loss of the BamHI L-M site which was shown to lie within coding sequence for the large subunit of ribonucleotide reductase. This particular mutation may therefore be selected for by passage in RK13 cells.
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Publication Date: 1994-01-01 PubMed ID: 8279952DOI: 10.1007/BF01379115Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers examined differences within and between strains of equine herpesvirus type 1 (EHV-1), finding variation caused by specific DNA changes within the virus particles. They noticed that a particular mutation linked to ribonucleotide reductase was associated with many of the strains, suggesting that this mutation could be selected for during the virus’s development.

Overview of Research Methodology

  • The researchers used restriction enzyme digests to analyse DNA from 22 different types of unselected EHV-1.
  • They employed cloned DNA fragments that spanned the entire genome to investigate hybridization.

Findings on Inter-strain Variation

  • Despite the close genetic relationships between the different isolates of the EHV-1, there was a small degree of inter-strain variation discovered.
  • This means that there were minor differences between different strains of the same virus, beyond the expected similarities due to shared gene sequences.

Intra-strain Variation and Consequences

  • In addition to variation found between different strains, unique discrepancies were found within the same strains as well.
  • These intra-strain variations were caused by a loss of specific restriction endonuclease sites in the DNA present in some of the virus particles within the same viral stock.

Detection of Common Mutation

  • One particular variation was observed in 15 of the strains studied, which involved a loss of the BamHI L-M site.
  • This site was found to lie within the coding sequence responsible for the large subunit of ribonucleotide reductase.
  • Ribonucleotide reductase is an enzyme crucial for DNA synthesis and repair, implying that mutations in its coding sequence could profoundly impact the viral lifecycle.

Possible Role of RK13 Cells

  • The research suggests that this common mutation might be selectively favored or “selected for” during passage of the virus in RK13 cells.
  • RK13 cells are rabbit kidney epithelial cells frequently used in virological research. They may enhance the propagation of this mutation, which may provide a survival or replication advantage to the virus.

Cite This Article

APA
Bonass WA, Hudson WA, Elton DM, Killington RA, Halliburton IW. (1994). Inter- and intra-strain genomic variation in equine herpesvirus type 1 isolates. Arch Virol, 134(1-2), 169-178. https://doi.org/10.1007/BF01379115

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 134
Issue: 1-2
Pages: 169-178

Researcher Affiliations

Bonass, W A
  • Department of Microbiology, University of Leeds, U.K.
Hudson, W A
    Elton, D M
      Killington, R A
        Halliburton, I W

          MeSH Terms

          • Amino Acid Sequence
          • Animals
          • Base Sequence
          • Blotting, Southern
          • Cell Line
          • Cloning, Molecular
          • DNA Probes
          • DNA, Viral
          • Genetic Variation
          • Genome, Viral
          • Herpesvirus 1, Equid / genetics
          • Horses
          • Molecular Sequence Data
          • Restriction Mapping

          Grant Funding

          • Wellcome Trust

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          Citations

          This article has been cited 1 times.
          1. Bryant NA, Wilkie GS, Russell CA, Compston L, Grafham D, Clissold L, McLay K, Medcalf L, Newton R, Davison AJ, Elton DM. Genetic diversity of equine herpesvirus 1 isolated from neurological, abortigenic and respiratory disease outbreaks. Transbound Emerg Dis 2018 Jun;65(3):817-832.
            doi: 10.1111/tbed.12809pubmed: 29423949google scholar: lookup
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