Interleukin-1 (IL-1) system gene expression in granulosa cells: kinetics during terminal preovulatory follicle maturation in the mare.
Abstract: A growing body of evidences suggests that the ovary is a site of inflammatory reactions, and thus, ovarian cells could represent sources and targets of the interleukin-1 (IL-1) system. The purpose of this study was to examine the IL-1 system gene expressions in equine granulosa cells, and to study the IL-1beta content in follicular fluid during the follicle maturation. For this purpose, granulosa cells and follicular fluids were collected from the largest follicle at the early dominance stage (diameter 24 +/- 3 mm) or during the preovulatory maturation phase, at T0 h, T6 h, T12 h, T24 h and T34 h after induction of ovulation. Cells were analysed by RT-PCR and follicular fluids were studied by gel electrophoresis and immunoblotting. Results: We demonstrated that interleukin-1beta (IL-1beta), interleukin-1 receptor 2 (IL-1R2) and interleukin-1 receptor antagonist (IL-1RA) genes are expressed in equine granulosa cells. We observed that the IL-1beta and IL-1RA mRNA content changed in granulosa cells during the terminal follicular maturation whereas IL-1R2 mRNA did not vary. In follicular fluid, IL-1beta content fluctuated few hours after induction of ovulation. Conclusions: The expression of IL-1beta gene in granulosa cells and the follicular fluid IL-1beta content seem to be regulated by gonadotropins suggesting that IL-1beta could be an intermediate paracrine factor involved in ovulation.
Publication Date: 2003-05-16 PubMed ID: 12803652PubMed Central: PMC166130DOI: 10.1186/1477-7827-1-42Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research examines the expression of interleukin-1 (IL-1) system genes in equine granulosa cells and their role in the maturation of preovulatory follicles in mares.
Objective and Methodology
- The study aimed to investigate the IL-1 system gene expressions in horse granulosa cells and to track the IL-1β content in follicular fluid during the follicle maturation process.
- The researchers collected granulosa cells and follicular fluids from the most significant follicle at the early dominance phase or during the preovulatory maturation phase at specific intervals after inaugurating ovulation.
- They used technology like RT-PCR for cellular analysis and gel electrophoresis and immunoblotting to study the follicular fluids.
Results and Observations
- The scientists discovered that the genes for interleukin-1β (IL-1β), interleukin-1 receptor 2 (IL-1R2), and interleukin-1 receptor antagonist (IL-1RA) are expressed in equine granulosa cells.
- The content of IL-1β and IL-1RA mRNA in granulosa cells changed during the terminal follicular maturation, but the IL-1R2 mRNA content remained static.
- The IL-1β content in the follicular fluid showed fluctuations a few hours post ovulation induction.
Conclusions
- The study deduced that both the expression of the IL-1β gene in granulosa cells and the IL-1β content in the follicular fluid appear to be guided by gonadotropins. This points towards a potential role of IL-1β as a paracrine factor assisting the process of ovulation.
Cite This Article
APA
Martoriati A, Gérard N.
(2003).
Interleukin-1 (IL-1) system gene expression in granulosa cells: kinetics during terminal preovulatory follicle maturation in the mare.
Reprod Biol Endocrinol, 1, 42.
https://doi.org/10.1186/1477-7827-1-42 Publication
Researcher Affiliations
- Reproduction Equine, Physiologie de la Reproduction et des Comportements, INRA UMR6073, 37380 Nouzilly, France. martoria@tours.inra.fr
MeSH Terms
- Animals
- Blotting, Western
- Body Fluids / chemistry
- Female
- Gene Expression Regulation
- Gonadotropins, Pituitary / physiology
- Granulosa Cells / metabolism
- Horses / genetics
- Horses / physiology
- Interleukin-1 / biosynthesis
- Interleukin-1 / genetics
- Ovarian Follicle / chemistry
- Ovulation / physiology
- Receptors, Interleukin-1 / biosynthesis
- Receptors, Interleukin-1 / genetics
- Receptors, Interleukin-1 Type II
- Reverse Transcriptase Polymerase Chain Reaction
References
This article includes 51 references
Citations
This article has been cited 10 times.- Lin X, Tong X, Zhang Y, Gu W, Huang Q, Zhang Y, Zhuo F, Zhao F, Jin X, Li C, Huang D, Zhang S, Dai Y. Decreased Expression of EZH2 in Granulosa Cells Contributes to Endometriosis-Associated Infertility by Targeting IL-1R2. Endocrinology 2022 Dec 19;164(2).
- Piccinni MP, Vicenti R, Logiodice F, Fabbri R, Kullolli O, Pallecchi M, Paradisi R, Danza G, Macciocca M, Lombardelli L, Seracchioli R. Description of the Follicular Fluid Cytokine and Hormone Profiles in Human Physiological Natural Cycles. J Clin Endocrinol Metab 2021 Jan 23;106(2):e721-e738.
- Popovic M, Sartorius G, Christ-Crain M. Chronic low-grade inflammation in polycystic ovary syndrome: is there a (patho)-physiological role for interleukin-1?. Semin Immunopathol 2019 Jul;41(4):447-459.
- Lv Y, Zhao SG, Lu G, Leung CK, Xiong ZQ, Su XW, Ma JL, Chan WY, Liu HB. Identification of reference genes for qRT-PCR in granulosa cells of healthy women and polycystic ovarian syndrome patients. Sci Rep 2017 Jul 31;7(1):6961.
- Burnik Papler T, Vrtacnik Bokal E, Maver A, Kopitar AN, Lovrečić L. Transcriptomic Analysis and Meta-Analysis of Human Granulosa and Cumulus Cells. PLoS One 2015;10(8):e0136473.
- Uri-Belapolsky S, Shaish A, Eliyahu E, Grossman H, Levi M, Chuderland D, Ninio-Many L, Hasky N, Shashar D, Almog T, Kandel-Kfir M, Harats D, Shalgi R, Kamari Y. Interleukin-1 deficiency prolongs ovarian lifespan in mice. Proc Natl Acad Sci U S A 2014 Aug 26;111(34):12492-7.
- Bonnet A, Bevilacqua C, Benne F, Bodin L, Cotinot C, Liaubet L, Sancristobal M, Sarry J, Terenina E, Martin P, Tosser-Klopp G, Mandon-Pepin B. Transcriptome profiling of sheep granulosa cells and oocytes during early follicular development obtained by laser capture microdissection. BMC Genomics 2011 Aug 18;12:417.
- Caillaud M, Duchamp G, Gérard N. In vivo effect of interleukin-1beta and interleukin-1RA on oocyte cytoplasmic maturation, ovulation, and early embryonic development in the mare. Reprod Biol Endocrinol 2005 Jun 22;3:26.
- Voros C, Papadimas G, Mavrogianni D, Koulakmanidis AM, Athanasiou D, Bananis K, Athanasiou A, Athanasiou A, Papapanagiotou I, Vaitsis D, Tsimpoukelis C, Daskalaki MA, Topalis V, Theodora M, Thomakos N, Chatzinikolaou F, Antsaklis P, Loutradis D, Menenakos E, Daskalakis G. Molecular Signatures of Obesity-Associated Infertility in Polycystic Ovary Syndrome: The Emerging Role of Exosomal microRNAs and Non-Coding RNAs. Genes (Basel) 2025 Sep 17;16(9).
- Banerjee S, Cooney LG, Stanic AK. Immune Dysfunction in Polycystic Ovary Syndrome. Immunohorizons 2023 May 1;7(5):323-332.
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