Interleukin-1 receptor antagonist expression in the equine endometrium during the peri-implantation period.
Abstract: To identify factors involved in the establishment of pregnancy in the mare, endometrium was collected from day 13 (day 0=day of ovulation) cyclic and day 13, 19, and 25 pregnant animals. From initial cDNA subtraction studies, interleukin-1 receptor antagonist (IL-1RN) mRNA was found as a candidate molecule expressed uniquely in the pregnant endometrium. Expression of IL-1RN mRNA was markedly increased in day 19 and 25 gravid endometrium. In situ hybridization analysis revealed that IL-1RN mRNA was localized to the glandular epithelium. Interleukin-1 receptor antagonist (IL-1RN) protein was found in the extracts of day 25 gravid endometrium and was immunochemically localized to the glandular epithelium/luminal cavity of the pregnant uterus. High concentrations of estradiol-17beta (E(2)) were detected in day 25 conceptuses. Concentrations of E(2) were higher in the gravid endometrial portion than in other endometrial regions. On the other hand, progesterone concentrations did not differ among endometrial samples analyzed. Furthermore, the expression of IL-1RN mRNA was up-regulated in endometrium culture samples treated with 10 ng/mL E(2) and 10 ng/mL progesterone. In the analysis of related gene expression, increased amounts of IL-1alpha and IL-6 mRNA were also found in the day 25 gravid endometrium; however, these expressions in endometrial culture samples were not up-regulated by the steroid treatment. These results indicate that expression of IL-1RN in the endometrium is likely regulated by E(2) and progesterone and suggest that IL-1RN regulates the degree of IL-1 signal transduction and thereby plays an important role in the establishment of equine pregnancy.
Publication Date: 2009-01-07 PubMed ID: 19157767DOI: 10.1016/j.domaniend.2008.11.006Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article examines the impact of Interleukin-1 receptor antagonist (IL-1RN) in the early stages of horse pregnancy. It finds evidence that IL-1RN, regulated by hormones estradiol and progesterone, could play a significant role in this process.
Collection and Analysis of Endometrium Samples
- The scientists collected endometrium samples from both pregnant and non-pregnant horses. The days of collection corresponded to day 13 (with day zero being the day of ovulation), day 19, and day 25 of the animal’s pregnancy. Samples from non-pregnant horses were also collected on day 13 of their ovulation cycle for comparative purposes.
- Initial cDNA subtraction studies helped identify IL-1RN mRNA as a molecule unique to pregnant endometrium samples.
Expression of IL-1RN
- The research found that the production of IL-1RN mRNA, the genetic instruction to build IL-1RN protein, increased dramatically on day 19 and 25 of pregnancy in horses.
- In-situ hybridization analysis revealed that the mRNA was localized to the glandular epithelium of the pregnant uterus.
- The IL-1RN protein was discovered through extraction from day 25 samples and later tested for with immunochemical localization that pointed to the glandular epithelium and luminal cavity of the horse’s pregnant uterus.
Hormonal Influence
- High levels of estradiol (E(2)) hormone were detected in conceptuses of the horses on day 25 of their pregnancy.
- Estradiol concentrations were higher in the pregnant portion of the endometrium, compared to other regions. Progesterone levels, however, showed no similar variations across different samples.
- The addition of E(2) and progesterone to endometrial culture samples increased IL-1RN mRNA expression, implying that these hormones regulate IL-1RN.
Related Gene Expressions
- The researchers observed increased expressions of IL-1alpha and IL-6 mRNA (other types of messenger RNA) in the day 25 pregnant endometrium. Unlike IL-1RN, however, these increases did not align with steroid treatment.
Significance of IL-1RN in Pregnancy
- Going by the results, the researchers believe that IL-1RN expression, as controlled by estradiol and progesterone, might regulate IL-1 signal transduction, which has important implications for the onset of horse pregnancy.
Cite This Article
APA
Haneda S, Nagaoka K, Nambo Y, Kikuchi M, Nakano Y, Matsui M, Miyake Y, Macleod JN, Imakawa K.
(2009).
Interleukin-1 receptor antagonist expression in the equine endometrium during the peri-implantation period.
Domest Anim Endocrinol, 36(4), 209-218.
https://doi.org/10.1016/j.domaniend.2008.11.006 Publication
Researcher Affiliations
- The United Graduate School of Veterinary Sciences, Gifu University, 501-1193 Gifu, Japan.
MeSH Terms
- Analysis of Variance
- Animals
- Embryo Implantation / physiology
- Endometrium / metabolism
- Estradiol / physiology
- Female
- Horses
- In Vitro Techniques
- Interleukin 1 Receptor Antagonist Protein / genetics
- Interleukin 1 Receptor Antagonist Protein / metabolism
- Interleukins / metabolism
- Pregnancy
- Pregnancy Maintenance / physiology
- Pregnancy, Animal / physiology
- RNA, Messenger / analysis
- Receptors, Interleukin-1 / metabolism
- Uterus / metabolism
Citations
This article has been cited 10 times.- Braucke AFGV, Frederiksen NL, Berg LC, Aarsvold S, Müller FC, Boesen MP, Lindegaard C. Identification and Quantification of Transient Receptor Potential Vanilloid 1 (TRPV1) in Equine Articular Tissue.. Animals (Basel) 2020 Mar 18;10(3).
- Satoh M, Higuchi T, Inoue S, Gotoh T, Murase H, Nambo Y. Factors affecting the prognosis for uterine torsion: the effect of treatment based on measurements of serum progesterone and estradiol concentrations after surgery.. J Equine Sci 2017;28(4):163-167.
- Griffith OW, Chavan AR, Protopapas S, Maziarz J, Romero R, Wagner GP. Embryo implantation evolved from an ancestral inflammatory attachment reaction.. Proc Natl Acad Sci U S A 2017 Aug 8;114(32):E6566-E6575.
- Haneda S, Nagaoka K, Nambo Y, Kikuchi M, Nakano Y, Li J, Matsui M, Miyake YI, Imakawa K. Expression of uterine lipocalin 2 and its receptor during early- to mid-pregnancy period in mares.. J Reprod Dev 2017 Apr 21;63(2):127-133.
- Murase H, Niwa H, Katayama Y, Sato F, Hada T, Nambo Y. A clinical case of equine fungal placentitis with reference to hormone profiles and ultrasonography.. J Equine Sci 2015;26(4):129-33.
- Andreassen SM, Berg LC, Nielsen SS, Kristensen AT, Jacobsen S. mRNA expression of genes involved in inflammation and haemostasis in equine fibroblast-like synoviocytes following exposure to lipopolysaccharide, fibrinogen and thrombin.. BMC Vet Res 2015 Jun 27;11:141.
- Tachibana Y, Sakurai T, Bai H, Shiota K, Nambo Y, Nagaoka K, Imakawa K. RNA-seq analysis of equine conceptus transcripts during embryo fixation and capsule disappearance.. PLoS One 2014;9(12):e114414.
- Szóstek AZ, Galvão AM, Hojo T, Okuda K, Skarzynski DJ. Interleukins affect equine endometrial cell function: modulatory action of ovarian steroids.. Mediators Inflamm 2014;2014:208103.
- Tachibana Y, Nakano Y, Nagaoka K, Kikuchi M, Nambo Y, Haneda S, Matsui M, Miyake Y, Imakawa K. Expression of endometrial immune-related genes possibly functioning during early pregnancy in the mare.. J Reprod Dev 2013;59(1):85-91.
- Walker CG, Meier S, Littlejohn MD, Lehnert K, Roche JR, Mitchell MD. Modulation of the maternal immune system by the pre-implantation embryo.. BMC Genomics 2010 Aug 13;11:474.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
