Interleukin-8 concentration and neutrophil chemotactic activity in bronchoalveolar lavage fluid of horses with chronic obstructive pulmonary disease following exposure to hay.
Abstract: To analyze effects of hay dust exposure on interleukin-8 (IL-8) concentration, percentage of neutrophils, and neutrophil chemotactic activity in bronchoalveolar lavage fluid (BALF) of horses with chronic obstructive pulmonary disease (COPD). Methods: 16 healthy horses and 29 horses with COPD. Methods: IL-8 concentration, percentage of neutrophils, and neutrophil chemotactic activity in BALF were measured. Values were analyzed with respect to hay dust exposure. These variables were also measured in 5 asymptomatic horses with COPD after the induction of clinical signs by changing feed from silage to hay. Results: L-8 concentrations and chemotactic activity in BALF were greater in horses with COPD, compared with healthy horses, and greater in horses with COPD exposed to hay dust, compared with nonexposed affected horses. An increase in IL-8 concentration accompanied by an increase in percentage of neutrophils in BALF and development of clinical signs of COPD were induced in asymptomatic horses with COPD by changing feed from silage to hay. Conclusions: Exposure of horses with COPD to hay dust components resulted in an increase in IL-8 secretion at the bronchoalveolar surface. This chemokine may play a role in the pathogenesis of COPD, because it causes neutrophil accumulation in the bronchoalveolar space. Our results underscore the importance of eliminating dust sources for the treatment and prevention of COPD in horses.
Publication Date: 2000-12-07 PubMed ID: 11108181DOI: 10.2460/ajvr.2000.61.1369Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research investigates how hay dust exposure affects the concentration of Interleukin-8 and activity of neutrophils in the bronchoalveolar lavage fluid of horses with chronic obstructive pulmonary disease.
Study Overview
- This study assessed the effect of hay dust on the concentration of Interleukin-8 (IL-8), the percentage of neutrophils and the activity of these neutrophils in the Broncho Alveolar Lavage Fluid (BALF) of horses with Chronic Obstructive Pulmonary Disease (COPD).
- The research involved 45 horses in total, 16 of which were healthy and 29 had COPD.
Methodology
- The researchers measured the IL-8 concentration, neutrophil percentage, and their activity in the BALF as variables in response to hay dust exposure.
- These variables were also measured for 5 asymptomatic horses with COPD, where clinical signs of COPD were intentionally induced by changing their feed from silage to hay.
Results
- The results indicated that the concentration of IL-8 and the activity of neutrophils were significantly increased in horses with COPD as compared to healthy horses.
- The increase was even more pronounced in horses with COPD that were exposed to hay dust compared to the ones that were not exposed.
- The symptomatic induction in asymptomatic COPD horses by changing their feed led to an increase in IL-8 concentration, an increase in the percentage of neutrophils, and the development of COPD clinical signs.
Conclusion and Implications
- The research concluded that exposure to hay dust in horses with COPD resulted in an increased secretion of IL-8, a chemokine, at the bronchoalveolar surface.
- Because IL-8 causes neutrophil accumulation in the bronchoalveolar space, this chemokine could play a role in the pathogenesis of COPD.
- The findings emphasize the importance of removing dust sources to help manage and prevent COPD in horses.
Cite This Article
APA
Franchini M, Gill U, von Fellenberg R, Bracher VD.
(2000).
Interleukin-8 concentration and neutrophil chemotactic activity in bronchoalveolar lavage fluid of horses with chronic obstructive pulmonary disease following exposure to hay.
Am J Vet Res, 61(11), 1369-1374.
https://doi.org/10.2460/ajvr.2000.61.1369 Publication
Researcher Affiliations
- Institute of Veterinary Physiology, Division of Applied Physiology, University of Zurich, Switzerland.
MeSH Terms
- Animal Feed
- Animals
- Bronchoalveolar Lavage Fluid / chemistry
- Bronchoalveolar Lavage Fluid / immunology
- Chemotaxis, Leukocyte / immunology
- Dust / adverse effects
- Horse Diseases / immunology
- Horses
- Housing, Animal
- Interleukin-8 / metabolism
- Leukocyte Count
- Lung Diseases, Obstructive / immunology
- Lung Diseases, Obstructive / veterinary
- Neutrophils / immunology
- Poaceae / adverse effects
- Poaceae / immunology
Citations
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- Adamič N, Prpar Mihevc S, Blagus R, Kramarič P, Krapež U, Majdič G, Viel L, Hoffman AM, Bienzle D, Vengust M. Effect of intrabronchial administration of autologous adipose-derived mesenchymal stem cells on severe equine asthma.. Stem Cell Res Ther 2022 Jan 21;13(1):23.
- Morini M, Peli A, Rinnovati R, Magazzù G, Romagnoli N, Spadari A, Pietra M. Immunohistochemical Expression of Neurokinin-A and Interleukin-8 in the Bronchial Epithelium of Horses with Severe Equine Asthma Syndrome during Asymptomatic, Exacerbation, and Remission Phase.. Animals (Basel) 2021 May 12;11(5).
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- Bright LA, Dittmar W, Nanduri B, McCarthy FM, Mujahid N, Costa LR, Burgess SC, Swiderski CE. Modeling the pasture-associated severe equine asthma bronchoalveolar lavage fluid proteome identifies molecular events mediating neutrophilic airway inflammation.. Vet Med (Auckl) 2019;10:43-63.
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