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Mediators of inflammation2014; 2014; 208103; doi: 10.1155/2014/208103

Interleukins affect equine endometrial cell function: modulatory action of ovarian steroids.

Abstract: The aim of the present study was to investigate the interaction between ovarian steroids, interleukins and prostaglandins (PG) in equine epithelial and stromal cells in vitro. In Experiment 1, cells were exposed to IL-1α (10 ng/mL), IL-1β (10 ng/mL) or IL-6 (10 ng/mL) for 24 h and cell proliferation was determined using MTT. In Experiment 2, cells were exposed to progesterone (P4; 10(-7) M); 17-β estradiol (E2; 10(-9) M) or P4+E2 for 24 h and later medium was replaced with a fresh one treated with IL-1α, IL-1β or IL-6 (10 ng/mL, each) for 24 h. The oxytocin (OT; 10(-7) M) was used as a positive control. In Experiment 3, cells were exposed to P4 (10(-7) M), E2 (10(-9) M) or P4+E2 for 24 h and the IL receptor mRNAs transcription was determined using Real-time PCR. Prostaglandins concentration was determined using the direct enzyme immunoassay (EIA) method. Our findings reveal a functional linking between ovarian steroids and IL-stimulated PG secretion by equine endometrial cells. This interaction could be one of the mechanisms responsible for endometrial local orchestrating events during the estrous cycle and early pregnancy.
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Publication Date: 2014-02-27 PubMed ID: 24719522PubMed Central: PMC3955593DOI: 10.1155/2014/208103Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research is studying the interaction between ovarian steroids, interleukins, and prostaglandins in horse endometrial cells. The study found a functional link between ovarian steroids and interleukin-stimulated prostaglandin secretion by these cells, suggesting this could be key in managing the estrous cycle and early pregnancy in horses.

Research Design and Experiments

  • The goal of the research was to understand how ovarian steroids, or sex hormones, interact with interleukins, types of signalling proteins, and prostaglandins, lipid compounds, in the endometrial cells in horses.
  • This was carried out through three different experiments, studying the effects of interleukins on cell proliferation, the effects of hormones on cells, and the expressions of interleukin receptors when cells were exposed to sex hormones.

Experiment 1: Cell Proliferation

  • The first experiment looked at the effect of interleukins on cell proliferation. Cells were exposed to Interleukins 1α, 1β, or 6 and then cell proliferation was determined using a cell proliferation assay.

Experiment 2: Role of Steroids

  • In the second experiment, cells were exposed to the hormones progesterone and estradiol separately and in conjunction, then the medium was replaced and treated with the same interleukins as in the first experiment. Oxytocin was also used as a positive control.

Experiment 3: Interleukin Receptor Expression

  • In the third experiment, cells were again exposed to the hormones, then the transcription of interleukin receptor mRNA was determined using real-time PCR.
  • This technique uses polymerase chain reaction to amplify selected parts of the DNA and quantify the amount of specific mRNA.
  • Last step of the research was to determine the concentration of prostaglandins using the direct enzyme immunoassay (EIA) method.

Findings and Implications

  • The studies ultimately found a functional link between sex hormones and Interleukin-stimulated PG secretion by equine endometrial cells.
  • This interaction could potentially be one of the mechanisms responsible for managing the estrous cycle and early pregnancy in horses.

Cite This Article

APA
Szóstek AZ, Galvão AM, Hojo T, Okuda K, Skarzynski DJ. (2014). Interleukins affect equine endometrial cell function: modulatory action of ovarian steroids. Mediators Inflamm, 2014, 208103. https://doi.org/10.1155/2014/208103

Publication

Mediators of inflammation
ISSN: 1466-1861
NlmUniqueID: 9209001
Country: United States
Language: English
Volume: 2014
Pages: 208103
PII: 208103

Researcher Affiliations

Szóstek, Anna Z
  • Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, 10-748 Olsztyn, Poland.
Galvão, Antonio M
  • Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, 10-748 Olsztyn, Poland.
Hojo, Takuo
  • Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, 10-748 Olsztyn, Poland.
Okuda, Kiyoshi
  • Laboratory of Reproductive Endocrinology Graduate School of Natural Science and Technology, Okayama University, 700-8530 Okayama, Japan.
Skarzynski, Dariusz J
  • Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, 10-748 Olsztyn, Poland.

MeSH Terms

  • Animals
  • Cell Proliferation
  • Cells, Cultured
  • Culture Media
  • Endometrium / cytology
  • Estradiol / pharmacology
  • Female
  • Gene Expression Regulation
  • Horses
  • Immunoenzyme Techniques
  • Interleukin-1alpha / pharmacology
  • Interleukin-1beta / pharmacology
  • Interleukin-6 / pharmacology
  • Interleukins / pharmacology
  • Ovary / metabolism
  • Progesterone / pharmacology
  • Prostaglandins / metabolism
  • RNA, Messenger / metabolism
  • Steroids / metabolism

References

This article includes 56 references
  1. Vernon MW, Zavy MT, Asquith RL, Sharp DC. Prostaglandin F2alpha in the equine endometrium: steroid modulation and production capacities during the estrous cycle and early pregnancy.. Biol Reprod 1981 Oct;25(3):581-9.
    pubmed: 6946841doi: 10.1095/biolreprod25.3.581google scholar: lookup
  2. Zavy MT, Vernon MW, Asquith RL, Bazer FW, Sharp DC. Effect of exogenous gonadal steroids and pregnancy on uterine luminal prostaglandin F in mares.. Prostaglandins 1984 Feb;27(2):311-20.
    pubmed: 6718755doi: 10.1016/0090-6980(84)90082-0google scholar: lookup
  3. Galvão A, Valente L, Skarzynski DJ, Szóstek A, Piotrowska-Tomala K, Rebordão MR, Mateus L, Ferreira-Dias G. Effect of cytokines and ovarian steroids on equine endometrial function: an in vitro study.. Reprod Fertil Dev 2013;25(7):985-97.
    pubmed: 23075812doi: 10.1071/rd12153google scholar: lookup
  4. Szóstek AZ, Galvão AM, Ferreira-Dias GM, Skarzynski DJ. Ovarian steroids affect prostaglandin production in equine endometrial cells in vitro.. J Endocrinol 2014 Mar;220(3):263-76.
    pubmed: 24481966doi: 10.1530/joe-13-0185google scholar: lookup
  5. Skarzynski DJ, Bah MM, Deptula KM, Woclawek-Potocka I, Korzekwa A, Shibaya M, Pilawski W, Okuda K. Roles of tumor necrosis factor-alpha of the estrous cycle in cattle: an in vivo study.. Biol Reprod 2003 Dec;69(6):1907-13.
    pubmed: 12904309doi: 10.1095/biolreprod.103.016212google scholar: lookup
  6. Vanderwall DK, Woods GL, Weber JA, Lichtenwalner AB. Corpus luteal function in nonpregnant mares following intrauterine administration of prostaglandin E(2) or estradiol-17beta.. Theriogenology 1994;42(7):1069-83.
    pubmed: 16727611doi: 10.1016/0093-691x(94)90855-9google scholar: lookup
  7. Jabbour HN, Boddy SC. Prostaglandin E2 induces proliferation of glandular epithelial cells of the human endometrium via extracellular regulated kinase 1/2-mediated pathway.. J Clin Endocrinol Metab 2003 Sep;88(9):4481-7.
    pubmed: 12970327doi: 10.1210/jc.2003-030297google scholar: lookup
  8. Milne SA, Jabbour HN. Prostaglandin (PG) F(2alpha) receptor expression and signaling in human endometrium: role of PGF(2alpha) in epithelial cell proliferation.. J Clin Endocrinol Metab 2003 Apr;88(4):1825-32.
    pubmed: 12679480doi: 10.1210/jc.2002-021368google scholar: lookup
  9. Tsujii M, DuBois RN. Alterations in cellular adhesion and apoptosis in epithelial cells overexpressing prostaglandin endoperoxide synthase 2.. Cell 1995 Nov 3;83(3):493-501.
    pubmed: 8521479doi: 10.1016/0092-8674(95)90127-2google scholar: lookup
  10. Kennedy TG, Gillio-Meina C, Phang SH. Prostaglandins and the initiation of blastocyst implantation and decidualization.. Reproduction 2007 Nov;134(5):635-43.
    pubmed: 17965253doi: 10.1530/rep-07-0328google scholar: lookup
  11. Tanikawa M, Acosta TJ, Fukui T, Murakami S, Korzekwa A, Skarzynski DJ, Piotrowska KK, Park CK, Okuda K. Regulation of prostaglandin synthesis by interleukin-1alpha in bovine endometrium during the estrous cycle.. Prostaglandins Other Lipid Mediat 2005 Dec;78(1-4):279-90.
    pubmed: 16303622doi: 10.1016/j.prostaglandins.2005.09.003google scholar: lookup
  12. Tanikawa M, Lee HY, Watanabe K, Majewska M, Skarzynski DJ, Park SB, Lee DS, Park CK, Acosta TJ, Okuda K. Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells.. J Endocrinol 2008 Dec;199(3):425-34.
    pubmed: 18824521doi: 10.1677/joe-08-0237google scholar: lookup
  13. Majewska M, Woclawek-Potocka I, Bah MM, Hapunik J, Piotrowska KK, Tasaki Y, Acosta TJ, Okuda K, Skarzynski DJ. Is interleukin-1alpha a luteotrophic or luteolytic agent in cattle?. Reproduction 2010 Mar;139(3):665-72.
    pubmed: 20032213doi: 10.1530/rep-09-0328google scholar: lookup
  14. Franczak A, Zmijewska A, Kurowicka B, Wojciechowicz B, Kotwica G. Interleukin 1β-induced synthesis and secretion of prostaglandin E₂ in the porcine uterus during various periods of pregnancy and the estrous cycle.. J Physiol Pharmacol 2010 Dec;61(6):733-42.
    pubmed: 21224505
  15. Franczak A, Zmijewska A, Kurowicka B, Wojciechowicz B, Petroff BK, Kotwica G. The effect of tumor necrosis factor α (TNFα), interleukin 1β (IL1β) and interleukin 6 (IL6) on endometrial PGF2α synthesis, metabolism and release in early-pregnant pigs.. Theriogenology 2012 Jan 1;77(1):155-65.
    pubmed: 21924479doi: 10.1016/j.theriogenology.2011.07.029google scholar: lookup
  16. Dinarello CA. The IL-1 family and inflammatory diseases.. Clin Exp Rheumatol 2002 Sep-Oct;20(5 Suppl 27):S1-13.
    pubmed: 14989423
  17. Heinrich PC, Behrmann I, Müller-Newen G, Schaper F, Graeve L. Interleukin-6-type cytokine signalling through the gp130/Jak/STAT pathway.. Biochem J 1998 Sep 1;334 ( Pt 2)(Pt 2):297-314.
    pmc: PMC1219691pubmed: 9716487doi: 10.1042/bj3340297google scholar: lookup
  18. Roberto da Costa RP, Serrão PM, Monteiro S, Pessa P, Silva JR, Ferreira-Dias G. Caspase-3-mediated apoptosis and cell proliferation in the equine endometrium during the oestrous cycle.. Reprod Fertil Dev 2007;19(8):925-32.
    pubmed: 18076824doi: 10.1071/rd06159google scholar: lookup
  19. Galvao AM, Ramilo DW, Skarzynski DJ, Lukasik K, Tramontano A, Mollo A, Mateus LM, Ferreira-Dias GM. Is FAS/Fas ligand system involved in equine corpus luteum functional regression?. Biol Reprod 2010 Dec;83(6):901-8.
    pubmed: 20720169doi: 10.1095/biolreprod.110.084699google scholar: lookup
  20. Ginther OJ. Reproductive Biology of the Mare: Basic and Applied Aspects. 2nd edition. Cross Plains, Wis, USA: Equiservices; 1992.
  21. Kenney RM. Cyclic and pathologic changes of the mare endometrium as detected by biopsy, with a note on early embryonic death.. J Am Vet Med Assoc 1978 Feb 1;172(3):241-62.
    pubmed: 621166
  22. Szóstek AZ, Siemieniuch MJ, Galvão AM, Lukasik K, Zieba D, Ferreira-Dias GM, Skarzynski DJ. Effects of cell storage and passage on basal and oxytocin-regulated prostaglandin secretion by equine endometrial epithelial and stromal cells.. Theriogenology 2012 May;77(8):1698-708.
    pubmed: 22357062doi: 10.1016/j.theriogenology.2011.12.015google scholar: lookup
  23. Szóstek AZ, Lukasik K, Galvão AM, Ferreira-Dias GM, Skarzynski DJ. Impairment of the interleukin system in equine endometrium during the course of endometrosis.. Biol Reprod 2013 Oct;89(4):79.
    pubmed: 23946535doi: 10.1095/biolreprod.113.109447google scholar: lookup
  24. Zhao S, Fernald RD. Comprehensive algorithm for quantitative real-time polymerase chain reaction.. J Comput Biol 2005 Oct;12(8):1047-64.
    pmc: PMC2716216pubmed: 16241897doi: 10.1089/cmb.2005.12.1047google scholar: lookup
  25. Uenoyama Y, Hattori S, Miyake M, Okuda K. Up-regulation of oxytocin receptors in porcine endometrium by adenosine 3',5'-monophosphate.. Biol Reprod 1997 Oct;57(4):723-8.
    pubmed: 9314572doi: 10.1095/biolreprod57.4.723google scholar: lookup
  26. Skarzynski DJ, Piotrowska KK, Bah MM, Korzekwa A, Woclawek-Potocka I, Sawai K, Okuda K. Effects of exogenous tumour necrosis factor-alpha on the secretory function of the bovine reproductive tract depend on tumour necrosis factor-alpha concentrations.. Reprod Domest Anim 2009 Jun;44(3):371-9.
    pubmed: 18564315doi: 10.1111/j.1439-0531.2007.01016.xgoogle scholar: lookup
  27. Ciereszko R, Opałka M, Kamińska B, Wojtczak M, Okrasa S, Dusza L. Luteotrophic action of prolactin during the early luteal phase in pigs: the involvement of protein kinases and phosphatases.. Reprod Biol 2001 Nov;1(2):62-83.
    pubmed: 14666168
  28. Fitko R, Kucharski J, Szlezyngier B, Jana B. The concentration of GnRH in hypothalamus, LH and FSH in pituitary, LH, PRL and sex steroids in peripheral and ovarian venous plasma of hypo- and hyperthyroid, cysts-bearing gilts.. Anim Reprod Sci 1996 Dec 2;45(1-2):123-38.
    pubmed: 9227918doi: 10.1016/s0378-4320(96)01568-0google scholar: lookup
  29. Szafrańska B, Ziecik A, Okrasa S. Primary antisera against selected steroids or proteins and secondary antisera against gamma-globulins--an available tool for studies of reproductive processes.. Reprod Biol 2002 Jul;2(2):187-204.
    pubmed: 14666157
  30. Haneda S, Nagaoka K, Nambo Y, Kikuchi M, Nakano Y, Matsui M, Miyake Y, Macleod JN, Imakawa K. Interleukin-1 receptor antagonist expression in the equine endometrium during the peri-implantation period.. Domest Anim Endocrinol 2009 May;36(4):209-18.
    pubmed: 19157767doi: 10.1016/j.domaniend.2008.11.006google scholar: lookup
  31. Bany BM, Kennedy TG. Interleukin-1 alpha regulates prostaglandin production and cyclooxygenase activity in sensitized rat endometrial stromal cells in vitro.. Biol Reprod 1995 Jul;53(1):126-32.
    pubmed: 7669843doi: 10.1095/biolreprod53.1.126google scholar: lookup
  32. Chen DB, Yang ZM, Hilsenrath R, Le SP, Harper MJ. Stimulation of prostaglandin (PG) F2 alpha and PGE2 release by tumour necrosis factor-alpha and interleukin-1 alpha in cultured human luteal phase endometrial cells.. Hum Reprod 1995 Oct;10(10):2773-80.
    pubmed: 8567812doi: 10.1093/oxfordjournals.humrep.a135790google scholar: lookup
  33. Tamura M, Sebastian S, Yang S, Gurates B, Fang Z, Bulun SE. Interleukin-1beta elevates cyclooxygenase-2 protein level and enzyme activity via increasing its mRNA stability in human endometrial stromal cells: an effect mediated by extracellularly regulated kinases 1 and 2.. J Clin Endocrinol Metab 2002 Jul;87(7):3263-73.
    pubmed: 12107235doi: 10.1210/jcem.87.7.8594google scholar: lookup
  34. Huang JC, Liu DY, Yadollahi S, Wu KK, Dawood MY. Interleukin-1 beta induces cyclooxygenase-2 gene expression in cultured endometrial stromal cells.. J Clin Endocrinol Metab 1998 Feb;83(2):538-41.
    pubmed: 9467571doi: 10.1210/jcem.83.2.4533google scholar: lookup
  35. Betts JG, Hansen PJ. Regulation of prostaglandin secretion from epithelial and stromal cells of the bovine endometrium by interleukin-1 beta, interleukin-2, granulocyte-macrophage colony stimulating factor and tumor necrosis factor-alpha.. Life Sci 1992;51(14):1171-6.
    pubmed: 1518379doi: 10.1016/0024-3205(92)90520-ygoogle scholar: lookup
  36. Tsujii M, Kawano S, Tsuji S, Sawaoka H, Hori M, DuBois RN. Cyclooxygenase regulates angiogenesis induced by colon cancer cells.. Cell 1998 May 29;93(5):705-16.
    pubmed: 9630216doi: 10.1016/s0092-8674(00)81433-6google scholar: lookup
  37. Sales KJ, Katz AA, Howard B, Soeters RP, Millar RP, Jabbour HN. Cyclooxygenase-1 is up-regulated in cervical carcinomas: autocrine/paracrine regulation of cyclooxygenase-2, prostaglandin e receptors, and angiogenic factors by cyclooxygenase-1.. Cancer Res 2002 Jan 15;62(2):424-32.
    pmc: PMC2694304pubmed: 11809691
  38. Kaczmarek MM, Blitek A, Kaminska K, Bodek G, Zygmunt M, Schams D, Ziecik AJ. Assessment of VEGF-receptor system expression in the porcine endometrial stromal cells in response to insulin-like growth factor-I, relaxin, oxytocin and prostaglandin E2.. Mol Cell Endocrinol 2008 Sep 10;291(1-2):33-41.
    pubmed: 18562087doi: 10.1016/j.mce.2008.04.020google scholar: lookup
  39. Paria BC, Reese J, Das SK, Dey SK. Deciphering the cross-talk of implantation: advances and challenges.. Science 2002 Jun 21;296(5576):2185-8.
    pubmed: 12077405doi: 10.1126/science.1071601google scholar: lookup
  40. Dinchuk JE, Car BD, Focht RJ, Johnston JJ, Jaffee BD, Covington MB, Contel NR, Eng VM, Collins RJ, Czerniak PM. Renal abnormalities and an altered inflammatory response in mice lacking cyclooxygenase II.. Nature 1995 Nov 23;378(6555):406-9.
    pubmed: 7477380doi: 10.1038/378406a0google scholar: lookup
  41. Lim H, Paria BC, Das SK, Dinchuk JE, Langenbach R, Trzaskos JM, Dey SK. Multiple female reproductive failures in cyclooxygenase 2-deficient mice.. Cell 1997 Oct 17;91(2):197-208.
    pubmed: 9346237doi: 10.1016/s0092-8674(00)80402-xgoogle scholar: lookup
  42. Kennedy TG. Evidence for a role for prosaglandins in the initiation of blastocyst implantation in the rat.. Biol Reprod 1977 Apr;16(3):286-91.
    pubmed: 843558doi: 10.1095/biolreprod16.3.286google scholar: lookup
  43. Evans CA, Kennedy TG. The importance of prostaglandin synthesis for the initiation of blastocyst implantation in the hamster.. J Reprod Fertil 1978 Nov;54(2):255-61.
    pubmed: 722674doi: 10.1530/jrf.0.0540255google scholar: lookup
  44. Oettel M, Koch M, Kurischko A, Schubert K. Direct evidence for the involvement of prostaglandin F2 alpha in the first step of estrone-induced blastocyst implantation in the spayed rat.. Steroids 1979 Jan;33(1):1-8.
    pubmed: 452058doi: 10.1016/s0039-128x(79)80001-xgoogle scholar: lookup
  45. Arici A, Engin O, Attar E, Olive DL. Modulation of leukemia inhibitory factor gene expression and protein biosynthesis in human endometrium.. J Clin Endocrinol Metab 1995 Jun;80(6):1908-15.
    pubmed: 7775640doi: 10.1210/jcem.80.6.7775640google scholar: lookup
  46. Garcia-Lloret MI, Morrish DW, Wegmann TG, Honore L, Turner AR, Guilbert LJ. Demonstration of functional cytokine-placental interactions: CSF-1 and GM-CSF stimulate human cytotrophoblast differentiation and peptide hormone secretion.. Exp Cell Res 1994 Sep;214(1):46-54.
    pubmed: 8082747doi: 10.1006/excr.1994.1232google scholar: lookup
  47. Singer CF, Marbaix E, Lemoine P, Courtoy PJ, Eeckhout Y. Local cytokines induce differential expression of matrix metalloproteinases but not their tissue inhibitors in human endometrial fibroblasts.. Eur J Biochem 1999 Jan;259(1-2):40-5.
    pubmed: 9914473doi: 10.1046/j.1432-1327.1999.00001.xgoogle scholar: lookup
  48. Salamonsen LA, Nie G. Proteases at the endometrial-trophoblast interface: their role in implantation.. Rev Endocr Metab Disord 2002 May;3(2):133-43.
    pubmed: 12007290doi: 10.1023/a:1015407012559google scholar: lookup
  49. Semer D, Reisler K, MacDonald PC, Casey ML. Responsiveness of human endometrial stromal cells to cytokines.. Ann N Y Acad Sci 1991;622:99-110.
    pubmed: 2064213doi: 10.1111/j.1749-6632.1991.tb37854.xgoogle scholar: lookup
  50. Nishino E, Matsuzaki N, Masuhiro K, Kameda T, Taniguchi T, Takagi T, Saji F, Tanizawa O. Trophoblast-derived interleukin-6 (IL-6) regulates human chorionic gonadotropin release through IL-6 receptor on human trophoblasts.. J Clin Endocrinol Metab 1990 Aug;71(2):436-41.
    pubmed: 2116439doi: 10.1210/jcem-71-2-436google scholar: lookup
  51. Tamura M, Sebastian S, Yang S, Gurates B, Fang Z, Bulun SE. Interleukin-1beta elevates cyclooxygenase-2 protein level and enzyme activity via increasing its mRNA stability in human endometrial stromal cells: an effect mediated by extracellularly regulated kinases 1 and 2.. J Clin Endocrinol Metab 2002 Jul;87(7):3263-73.
    pubmed: 12107235doi: 10.1210/jcem.87.7.8594google scholar: lookup
  52. Choudhary S, Kumar A, Kale RK, Raisz LG, Pilbeam CC. Extracellular calcium induces COX-2 in osteoblasts via a PKA pathway.. Biochem Biophys Res Commun 2004 Sep 17;322(2):395-402.
    pubmed: 15325243doi: 10.1016/j.bbrc.2004.07.129google scholar: lookup
  53. Gray T, Nettesheim P, Loftin C, Koo JS, Bonner J, Peddada S, Langenbach R. Interleukin-1beta-induced mucin production in human airway epithelium is mediated by cyclooxygenase-2, prostaglandin E2 receptors, and cyclic AMP-protein kinase A signaling.. Mol Pharmacol 2004 Aug;66(2):337-46.
    pubmed: 15266025doi: 10.1124/mol.66.2.337google scholar: lookup
  54. Dai R, Phillips RA, Karpuzoglu E, Khan D, Ahmed SA. Estrogen regulates transcription factors STAT-1 and NF-kappaB to promote inducible nitric oxide synthase and inflammatory responses.. J Immunol 2009 Dec 1;183(11):6998-7005.
    pmc: PMC2782783pubmed: 19890039doi: 10.4049/jimmunol.0901737google scholar: lookup
  55. Kelly MJ, Lagrange AH, Wagner EJ, Rønnekleiv OK. Rapid effects of estrogen to modulate G protein-coupled receptors via activation of protein kinase A and protein kinase C pathways.. Steroids 1999 Jan-Feb;64(1-2):64-75.
    pubmed: 10323674doi: 10.1016/s0039-128x(98)00095-6google scholar: lookup
  56. Shingo AS, Kito S. Estradiol induces PKA activation through the putative membrane receptor in the living hippocampal neuron.. J Neural Transm (Vienna) 2005 Nov;112(11):1469-73.
    pubmed: 16245068doi: 10.1007/s00702-005-0371-8google scholar: lookup

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