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Home / Equine Research Bank / Am J Vet Res / Intra-abdominal hyaluronan concentration in peritoneal fluid...
American journal of veterinary research2011; 72(12); 1666-1673; doi: 10.2460/ajvr.72.12.1666

Intra-abdominal hyaluronan concentration in peritoneal fluid of horses with sudden signs of severe abdominal pain.

Abstract: To determine hyaluronan concentrations in peritoneal fluid from healthy horses and horses with sudden signs of severe abdominal pain and to identify the cellular sources of hyaluronan within the peritoneal cavity. Methods: 7 client-owned horses that were evaluated for sudden signs of severe abdominal pain, 6 healthy teaching horses, and 13 euthanized horses (11 with no abdominal disease and 2 that had undergone abdominal surgery 2 weeks previously for a different study). Methods: Abdominal fluid was collected from the client-owned and teaching horses. Hyaluronan concentrations were determined with an ELISA. Equine mesothelial cells were aseptically harvested from euthanized horses immediately after euthanasia, cultured, and processed for western blot immunoassays to detect expression of the following mesothelial cell markers: cytokeratins 8 and 18, vimentin, calretinin, mesothelin, and CD44. A reverse transcriptase-PCR assay was used to detect genetic expression of hyaluronan synthase-2 (HAS-2) from cultured and native equine tissue. Results: The mean ± SD abdominal hyaluronan concentration in peritoneal fluid from horses with signs of abdominal pain (1,203.3 ± 46.3 ng/mL) was significantly greater than that in healthy horses (228.4 ± 167.3 ng/mL). Harvested cells were maintained, and immunoblotting analyses confirmed expression of the mesothelial markers. Gene expression of HAS-2 from cultured mesothelial cells and fibroblasts was confirmed. Conclusions: Peritoneal hyaluronan concentration was much higher in horses with severe abdominal pain than in healthy horses. Cultured equine mesothelial cells and fibroblasts can produce hyaluronan through HAS-2. Future investigation should focus on establishing the effect of exogenous hyaluronan administration on mesothelial cell function in horses with abdominal disease.
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Publication Date: 2011-12-01 PubMed ID: 22126696PubMed Central: PMC4487920DOI: 10.2460/ajvr.72.12.1666Google Scholar: Lookup
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  • Journal Article
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  • Extramural
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research was aimed at understanding levels of hyaluronan, a substance involved in inflammation, in the abdominal fluid of horses that are healthy and those experiencing severe abdominal pain. The study also sought to identify the primary cells responsible for producing hyaluronan in the peritoneum, the lining of the abdominal cavity.

Research Methodology

  • Seven horses showing sudden signs of severe abdominal pain, six healthy horses, and 13 euthanized horses (11 without any abdominal disease and two that had undergone abdominal surgery) were used for the study.
  • Abdominal fluid samples were collected directly from the seven ill and six healthy horses.
  • The concentration of hyaluronan in these fluids was measured through enzyme-linked immunosorbent assays (ELISA), a common lab test for detecting and measuring antibodies in blood.
  • Cell samples were taken post-euthanasia from the 13 euthanized horses, which were then cultured or grown within the lab for further tests.
  • Using western blot immunoassays, a process used to separate and identify proteins, the cell samples were analyzed for the presence mesothelial cell markers. These cell “markers” are proteins that signal different types of cellular activity.
  • A polymerase chain reaction (PCR) test, used for making many copies of a small piece of DNA, was conducted to confirm the genetic expression of hyaluronan synthase-2 (HAS-2) – the enzyme responsible for hyaluronan production.

Results and Findings

  • Horses with severe abdominal pain had significantly higher hyaluronan concentrations in their abdominal fluids – around 1,203.3 ± 46.3 ng/mL, compared to healthy horses with 228.4 ± 167.3 ng/mL.
  • The cultured cells from the euthanized horses showed expression of mesothelial markers, indicating that these cells were likely the source of hyaluronan production.
  • The PCR assay confirmed the genetic expression of HAS-2 in the cultured and native equine tissues.

Conclusion and Further Research

  • The study concluded that horses with severe abdominal pain had much higher peritoneal hyaluronan concentrations than their healthy counterparts.
  • The cultured equine mesothelial cells and fibroblasts were discovered to be capable of producing hyaluronan through HAS-2.
  • The research suggests that future studies should investigate the impact of administering exogenous hyaluronan (externally sourced) on mesothelial cell function in horses suffering from abdominal disease.

Cite This Article

APA
Lillich JD, Ray-Miller W, Silver KS, Davis EG, Schultz BD. (2011). Intra-abdominal hyaluronan concentration in peritoneal fluid of horses with sudden signs of severe abdominal pain. Am J Vet Res, 72(12), 1666-1673. https://doi.org/10.2460/ajvr.72.12.1666

Publication

American journal of veterinary research
ISSN: 1943-5681
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 72
Issue: 12
Pages: 1666-1673

Researcher Affiliations

Lillich, James D
  • Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA. lillich@vet.k-state.edu
Ray-Miller, Wendy
    Silver, Kristopher S
      Davis, Elizabeth G
        Schultz, Bruce D

          MeSH Terms

          • Abdominal Pain / metabolism
          • Abdominal Pain / veterinary
          • Animals
          • Ascitic Fluid / chemistry
          • Ascitic Fluid / metabolism
          • Biomarkers / analysis
          • Biomarkers / blood
          • Blotting, Western / veterinary
          • Cells, Cultured
          • Enzyme-Linked Immunosorbent Assay / veterinary
          • Epithelial Cells / metabolism
          • Epithelium / metabolism
          • Fibroblasts / metabolism
          • Glucuronosyltransferase / metabolism
          • Horse Diseases / metabolism
          • Horses
          • Hyaluronic Acid / analysis
          • Hyaluronic Acid / blood
          • Hyaluronic Acid / metabolism
          • Peritoneal Cavity / cytology
          • Reverse Transcriptase Polymerase Chain Reaction / veterinary

          Grant Funding

          • P20 RR017686 / NCRR NIH HHS
          • P20 RR017686-09 / NCRR NIH HHS
          • P50 CA095060 / NCI NIH HHS
          • RR017686 / NCRR NIH HHS

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          Citations

          This article has been cited 1 times.
          1. Ludwig EK, Hobbs KJ, McKinney-Aguirre CA, Gonzalez LM. Biomarkers of Intestinal Injury in Colic. Animals (Basel) 2023 Jan 7;13(2).
            doi: 10.3390/ani13020227pubmed: 36670767google scholar: lookup
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