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Journal of assisted reproduction and genetics2019; 36(6); 1237-1250; doi: 10.1007/s10815-019-01460-7

Intrafollicular oocyte transfer in the horse: effect of autologous vs. allogeneic transfer and time of administration of ovulatory stimulus before transfer.

Abstract: To assess meiotic and developmental competence after transfer of immature cumulus-oocyte complexes (COCs) to the preovulatory follicles of mares (intrafollicular oocyte transfer (IFOT)). Methods: In Experiment 1, mares received an ovulatory stimulus at IFOT. Thirty hours later, COCs were recovered from the follicle, and mature oocytes underwent ICSI and embryo culture. In Experiments 2 and 3, autologous vs. allogeneic COCs were used. The mares were inseminated and embryos were recovered. In Experiment 3, the ovulatory stimulus was administered 9 h (autologous) and 15 h (allogeneic) before IFOT. In Experiment 4, only allogeneic COCs were used; the ovulatory stimulus was administered 9 or 15 h before IFOT. Excess embryos (autologous) and parentage-verified embryos (allogeneic) were considered IFOT-derived. Results: In Experiment 1, 36/54 IFOT oocytes (67%) were recovered, of which 56% were mature, vs. 49% of in vitro matured oocytes (P > 0.1). After ICSI, blastocyst rates were 25% and 18%, respectively (P > 0.1). In Experiment 2, 0/6 autologous and 2/6 allogeneic IFOT yielded IFOT-derived embryos. In Experiment 3, 0/7 autologous and 2/5 allogeneic IFOT yielded IFOT-derived embryos. The proportion of mares yielding IFOT-derived embryos was lower after autologous vs. allogeneic IFOT (0/13 vs. 4/11; P < 0.05). In Experiment 4, 1/8 9-h and 1/7 15-h IFOT yielded IFOT-derived embryos. Conclusions: Transferred oocytes mature within the follicle and can maintain developmental competence. Allogeneic IFOT was more efficient than was autologous IFOT. The time of ovulatory stimulation did not affect embryo yield. The IFOT procedure is still not repeatable enough to be recommended for clinical use.
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Publication Date: 2019-05-09 PubMed ID: 31073725PubMed Central: PMC6603247DOI: 10.1007/s10815-019-01460-7Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This is a study on intrafollicular oocyte transfer (IFOT), a reproductive technique in horses, which examined the effect of using own eggs versus donor eggs (autologous vs allogeneic transfer) and the timing of stimulating ovulation. The researchers found that the transferred eggs can mature within the follicle and maintain their ability to develop but allogeneic transfer was more effective. The timing of ovulation stimulation didn’t impact the yield of embryos. However, the IFOT procedure is not yet reliable for clinical use.

Experiment Design

  • The study involved four key experiments assessing different aspects influencing the efficiency of IFOT. In Experiment 1, the collected cumulus-oocyte complexes (COCs), which are structures needed for oocyte maturation, were stimulated for ovulation at the time of transfer. Thirty hours after this, COCS were recovered, and mature oocytes underwent intracytoplasmic sperm injection (ICSI) and embryo culture.
  • Experiments 2 and 3 involved the comparison of autologous and allogeneic (donor) COCs. In these experiments, the mares were inseminated, and embryos were collected.
  • In Experiment 3, the ovulatory stimulus was administered 9 hours and 15 hours prior to IFOT for autologous and allogeneic conditions respectively.
  • In Experiment 4, only allogeneic COCs were used, while the ovulatory stimulus was given either 9 or 15 hours before IFOT.

Results Summary

  • In the first experiment, they recovered 67% of the IFOT oocytes, with a 56% maturity rate and an embryo blastocyst formation rate of 25% after ICSI.
  • Experiments 2 and 3 showed that allogeneic IFOT yielded IFOT-derived embryos, while autologous IFOT did not. This suggested allogeneic IFOT was more efficient than autologous IFOT.
  • In Experiment 4, one out of several attempts in both 9-hour and 15-hour pre-stimulation conditions yielded IFOT-derived embryos. This concluded the timing of ovulatory stimulation did not impact the yield of embryos.

Conclusions

  • The study concluded that transferred oocytes can mature within the follicle and maintain their ability to develop into embryos, demonstrating the potential effectiveness of IFOT as a reproductive technique in horses.
  • Allogeneic IFOT was determined to be more efficient than autologous IFOT, suggesting a preference for donor eggs.
  • The time at which the mares were stimulated to ovulate did not significantly impact the yield of embryos from the process.
  • The researchers however noted that the IFOT procedure was not reliable and repeatable enough to be recommended for clinical veterinary use at this stage.

Cite This Article

APA
Martinez de Andino EV, Brom-de-Luna JG, Canesin HS, Rader K, Resende HL, Ripley AM, Love CC, Hinrichs K. (2019). Intrafollicular oocyte transfer in the horse: effect of autologous vs. allogeneic transfer and time of administration of ovulatory stimulus before transfer. J Assist Reprod Genet, 36(6), 1237-1250. https://doi.org/10.1007/s10815-019-01460-7

Publication

Journal of assisted reproduction and genetics
ISSN: 1573-7330
NlmUniqueID: 9206495
Country: Netherlands
Language: English
Volume: 36
Issue: 6
Pages: 1237-1250

Researcher Affiliations

Martinez de Andino, E V
  • Department of Large Animal Clinical Sciences, Texas A&M University, College Station, TX, 77843-4475, USA.
Brom-de-Luna, J G
  • Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 4466 TAMU, College Station, TX, 77843-4466, USA.
Canesin, H S
  • Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 4466 TAMU, College Station, TX, 77843-4466, USA.
Rader, K
  • Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 4466 TAMU, College Station, TX, 77843-4466, USA.
Resende, H L
  • Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 4466 TAMU, College Station, TX, 77843-4466, USA.
Ripley, A M
  • Department of Large Animal Clinical Sciences, Texas A&M University, College Station, TX, 77843-4475, USA.
Love, C C
  • Department of Large Animal Clinical Sciences, Texas A&M University, College Station, TX, 77843-4475, USA.
Hinrichs, K
  • Department of Large Animal Clinical Sciences, Texas A&M University, College Station, TX, 77843-4475, USA. khinrichs@cvm.tamu.edu.
  • Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 4466 TAMU, College Station, TX, 77843-4466, USA. khinrichs@cvm.tamu.edu.

MeSH Terms

  • Animals
  • Blastocyst / metabolism
  • Cumulus Cells / transplantation
  • Embryo Transfer
  • Embryo, Mammalian
  • Embryonic Development / genetics
  • Female
  • Horses
  • In Vitro Oocyte Maturation Techniques
  • Oocyte Retrieval
  • Oocytes / growth & development
  • Oogenesis / genetics
  • Ovarian Follicle / growth & development
  • Sperm Injections, Intracytoplasmic
  • Transplantation, Autologous

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Citations

This article has been cited 1 times.
  1. Teng M, Zhao M, Mu B, Lei A. Allogenic Follicular Fosterage Technology: Problems, Progress and Potential. Vet Sci 2024 Jun 17;11(6).
    doi: 10.3390/vetsci11060276pubmed: 38922023google scholar: lookup
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