FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Int J Stem Cells / Intramuscular Transplantation of Allogeneic Mesenchymal Stro...
International journal of stem cells2016; 9(2); 239-249; doi: 10.15283/ijsc16011

Intramuscular Transplantation of Allogeneic Mesenchymal Stromal Cells Derived from Equine Umbilical Cord.

Abstract: Mesenchymal stromal cells (MSCs) have great therapeutic potential, particularly in the process of tissue repair and immunomodulation through the secretion of biomolecules. Thus, the aim of this study was to evaluate the hypothesis that intramuscular transplantation of allogeneic MSCs obtained from equine umbilical cord (UC-MSCs) is safe, demonstrating that this is a suitable source of stem cells for therapeutic use. Results: For this, UC-MSCs were cultured, characterized and cryopreserved for future transplantation in six healthy mares. On day 0, transplantation of three million UC-MSCs diluted in Hank's Balanced Solution (HBSS) was performed on right and left sides of the rump muscle. As a control, HBSS injections were performed caudally in the same muscle. Muscle biopsies were obtained as a control 30 days before transplantation (D-30). The biopsies were collected again on day 2 (left side) and day 7 (right side) post transplantation and examined histologically. All procedures were preceded by ultrasound examination and blood sampling. Hematologic evaluation remained within normal limits and no differences were observed between time points (p>0.05). Ultrasound examination was suggestive of inflammation 48 hours after transplantation in both groups (control and treated). At histological evaluation it was found only discrete inflammation signals between D-30×D2 (p 0.05) between the groups at different time points. Conclusions: Equine UC-MSCs under the experimental conditions did not promote severe inflammation that causes tissue damage or lead to its rejection by the host organism and therefore has a good potential for clinical use.
Get Full Text
Publication Date: 2016-08-31 PubMed ID: 27572709PubMed Central: PMC5155720DOI: 10.15283/ijsc16011Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the use of Mesenchymal stromal cells (MSCs) derived from horse umbilical cords for tissue repair and immunomodulation. The researchers aimed to confirm if MSC transplantation via intramuscular injection is safe and a viable source of stem cells for therapeutic use. The study established that the procedure did not cause severe inflammation or tissue damage and is potentially suitable for clinical applications.

Methodology and Experimental Setup

  • The study started by culturing, characterising and cryopreserving MSCs obtained from equine umbilical cords for future transplantation into six healthy mares.
  • The researchers conducted the transplant on Day 0, injecting three million UC-MSCs into both sides of the rump muscle of each mare.
  • As a control, they also performed injections of Hank’s Balanced Solution (HBSS), an isotonic cell culture solution, into the same muscle area.
  • Muscle biopsies were taken as a control 30 days before transplantation (D-30) and again on day 2 (left side) and day 7 (right side) after transplantation for histological examination. The hematologic evaluation was also conducted, and ultrasound was used to monitor the procedure.

Findings and Conclusion

  • The hematologic evaluation showed no significant variation and remained within the normal limits, implying that the transplant procedure did not result in abnormal blood conditions or reactions.
  • An ultrasound examination revealed possible inflammation 48 hours after transplantation in both groups. However, on histological examination, only mild inflammation was observed between D-30 and D2 in the treatment group.
  • No statistical differences were noted in inflammation levels between the control and treatment groups at different times, suggesting that the transplantation of equine UC-MSCs did not significantly increase inflammation.
  • The overall findings illustrate that the transplantation of equine UC-MSCs did not trigger severe inflammation, tissue damage, or induce rejection by the host organism. This suggests that it could be a safe and effective method for therapeutic applications.

Cite This Article

APA
Dias MC, Landim-Alvarenga FD, de Moraes CN, da Costa LD, Geraldini CM, de Vasconcelos Machado VM, Maia L. (2016). Intramuscular Transplantation of Allogeneic Mesenchymal Stromal Cells Derived from Equine Umbilical Cord. Int J Stem Cells, 9(2), 239-249. https://doi.org/10.15283/ijsc16011

Publication

International journal of stem cells
ISSN: 2005-3606
NlmUniqueID: 101497587
Country: Korea (South)
Language: English
Volume: 9
Issue: 2
Pages: 239-249

Researcher Affiliations

Dias, Marianne Camargos
  • Department of Animal Reproduction and Veterinary Radiology, College of Veterinary Medicine and Animal Science, São Paulo State University, UNESP, Botucatu, SP, Brazil.
Landim-Alvarenga, Fernanda da Cruz
  • Department of Animal Reproduction and Veterinary Radiology, College of Veterinary Medicine and Animal Science, São Paulo State University, UNESP, Botucatu, SP, Brazil.
de Moraes, Carolina Nogueira
  • Department of Animal Reproduction and Veterinary Radiology, College of Veterinary Medicine and Animal Science, São Paulo State University, UNESP, Botucatu, SP, Brazil.
da Costa, Leonardo Dourado
  • Department of Veterinary Pathology, College of Veterinary Medicine and Animal Science, São Paulo State University, UNESP, Botucatu, SP, Brazil.
Geraldini, Caroline Medeiros
  • Department of Animal Reproduction and Veterinary Radiology, College of Veterinary Medicine and Animal Science, São Paulo State University, UNESP, Botucatu, SP, Brazil.
de Vasconcelos Machado, Vânia Maria
  • Department of Animal Reproduction and Veterinary Radiology, College of Veterinary Medicine and Animal Science, São Paulo State University, UNESP, Botucatu, SP, Brazil.
Maia, Leandro
  • Department of Animal Reproduction and Veterinary Radiology, College of Veterinary Medicine and Animal Science, São Paulo State University, UNESP, Botucatu, SP, Brazil.

References

This article includes 28 references
  1. Voswinkel J, Francois S, Gorin NC, Chapel A. Gastro-intestinal autoimmunity: preclinical experiences and successful therapy of fistulizing bowel diseases and gut Graft versus host disease by mesenchymal stromal cells.. Immunol Res 2013 Jul;56(2-3):241-8.
    doi: 10.1007/s12026-013-8397-8pubmed: 23564182google scholar: lookup
  2. Durando MM, Zarucco L, Schaer TP, Ross M, Reef VB. Pneumopericardium in a horse secondary to sternal bone marrow aspiration.. Equine Vet Educ 2010;18:75–79.
    doi: 10.1111/j.2042-3292.2006.tb00419.xgoogle scholar: lookup
  3. Paterson YZ, Rash N, Garvican ER, Paillot R, Guest DJ. Equine mesenchymal stromal cells and embryo-derived stem cells are immune privileged in vitro.. Stem Cell Res Ther 2014 Jul 30;5(4):90.
    doi: 10.1186/scrt479pmc: PMC4247727pubmed: 25080326google scholar: lookup
  4. Dyson SJ. Are mesenchymal progenitor cells set to revolutionise management of musculoskeletal injuries in the horse?. Vet J 2013 Sep;197(3):533-4.
    doi: 10.1016/j.tvjl.2013.05.018pubmed: 23778255google scholar: lookup
  5. Carrade DD, Affolter VK, Outerbridge CA, Watson JL, Galuppo LD, Buerchler S, Kumar V, Walker NJ, Borjesson DL. Intradermal injections of equine allogeneic umbilical cord-derived mesenchymal stem cells are well tolerated and do not elicit immediate or delayed hypersensitivity reactions.. Cytotherapy 2011 Nov;13(10):1180-92.
    doi: 10.3109/14653249.2011.602338pubmed: 21899391google scholar: lookup
  6. Carrade DD, Owens SD, Galuppo LD, Vidal MA, Ferraro GL, Librach F, Buerchler S, Friedman MS, Walker NJ, Borjesson DL. Clinicopathologic findings following intra-articular injection of autologous and allogeneic placentally derived equine mesenchymal stem cells in horses.. Cytotherapy 2011 Apr;13(4):419-30.
    doi: 10.3109/14653249.2010.536213pubmed: 21105841google scholar: lookup
  7. Kode JA, Mukherjee S, Joglekar MV, Hardikar AA. Mesenchymal stem cells: immunobiology and role in immunomodulation and tissue regeneration.. Cytotherapy 2009;11(4):377-91.
    doi: 10.1080/14653240903080367pubmed: 19568970google scholar: lookup
  8. Sohni A, Verfaillie CM. Mesenchymal stem cells migration homing and tracking.. Stem Cells Int 2013;2013:130763.
    doi: 10.1155/2013/130763pmc: PMC3806396pubmed: 24194766google scholar: lookup
  9. Pigott JH, Ishihara A, Wellman ML, Russell DS, Bertone AL. Inflammatory effects of autologous, genetically modified autologous, allogeneic, and xenogeneic mesenchymal stem cells after intra-articular injection in horses.. Vet Comp Orthop Traumatol 2013;26(6):453-60.
    doi: 10.3415/VCOT-13-01-0008pubmed: 24080668google scholar: lookup
  10. Maia L, da Cruz Landim-Alvarenga F, Taffarel MO, de Moraes CN, Machado GF, Melo GD, Amorim RM. Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses.. BMC Vet Res 2015 Mar 15;11:63.
    doi: 10.1186/s12917-015-0361-5pmc: PMC4369105pubmed: 25879519google scholar: lookup
  11. Lovati AB, Corradetti B, Lange Consiglio A, Recordati C, Bonacina E, Bizzaro D, Cremonesi F. Comparison of equine bone marrow-, umbilical cord matrix and amniotic fluid-derived progenitor cells.. Vet Res Commun 2011 Feb;35(2):103-21.
    doi: 10.1007/s11259-010-9457-3pubmed: 21193959google scholar: lookup
  12. Smith RK, Garvican ER, Fortier LA. The current 'state of play' of regenerative medicine in horses: what the horse can tell the human.. Regen Med 2014;9(5):673-85.
    doi: 10.2217/rme.14.42pubmed: 25372081google scholar: lookup
  13. Maia L, Landim-Alvarenga FC, Da Mota LS, De Assis Golim M, Laufer-Amorim R, De Vita B, Barberini DJ, Listoni AJ, De Moraes CN, Heckler MC, Amorim RM. Immunophenotypic, immunocytochemistry, ultrastructural, and cytogenetic characterization of mesenchymal stem cells from equine bone marrow.. Microsc Res Tech 2013 Jun;76(6):618-24.
    doi: 10.1002/jemt.22208pubmed: 23533133google scholar: lookup
  14. Mensing N, Gasse H, Hambruch N, Haeger JD, Pfarrer C, Staszyk C. Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse.. BMC Vet Res 2011 Aug 2;7:42.
    doi: 10.1186/1746-6148-7-42pmc: PMC3161857pubmed: 21810270google scholar: lookup
  15. Jain NC. Essentials of veterinary hematology. Philadelphia: Lea & Febiger; 1993. p. 417.
  16. Meyer DJ, Harvey JW. Veterinary laboratory medicine: interpretation & diagnosis. 2nd ed. Philadelphia: Sauders; 2004. p. 351.
  17. Padilha FGF, Suma R, Amorim RM. Avaliação prática da biópsia muscular percutânea por agulha em equinos.. Hora Vet 2008;28:29–32.
  18. Brasileiro JL, Fagundes DJ, Miiji LON, Oshima CTF, Teruya R, Marks G, Inouye CM, Santos MA. Isquemia e reperfusão de músculo sóleo de ratos sob ação da pentoxifilina.. J Vasc Bras 2007;6:50–63.
    doi: 10.1590/S1677-54492007000100008google scholar: lookup
  19. Corradetti B, Lange-Consiglio A, Barucca M, Cremonesi F, Bizzaro D. Size-sieved subpopulations of mesenchymal stem cells from intervascular and perivascular equine umbilical cord matrix.. Cell Prolif 2011 Aug;44(4):330-42.
    doi: 10.1111/j.1365-2184.2011.00759.xpmc: PMC6496711pubmed: 21645152google scholar: lookup
  20. Iacono E, Brunori L, Pirrone A, Pagliaro PP, Ricci F, Tazzari PL, Merlo B. Isolation, characterization and differentiation of mesenchymal stem cells from amniotic fluid, umbilical cord blood and Wharton's jelly in the horse.. Reproduction 2012 Apr;143(4):455-68.
    doi: 10.1530/REP-10-0408pubmed: 22274885google scholar: lookup
  21. De Schauwer C, Piepers S, Van de Walle GR, Demeyere K, Hoogewijs MK, Govaere JL, Braeckmans K, Van Soom A, Meyer E. In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry.. Cytometry A 2012 Apr;81(4):312-23.
    doi: 10.1002/cyto.a.22026pubmed: 22411893google scholar: lookup
  22. Ranera B, Lyahyai J, Romero A, Vázquez FJ, Remacha AR, Bernal ML, Zaragoza P, Rodellar C, Martín-Burriel I. Immunophenotype and gene expression profiles of cell surface markers of mesenchymal stem cells derived from equine bone marrow and adipose tissue.. Vet Immunol Immunopathol 2011 Nov 15;144(1-2):147-54.
    doi: 10.1016/j.vetimm.2011.06.033pubmed: 21782255google scholar: lookup
  23. Schnabel LV, Pezzanite LM, Antczak DF, Felippe MJ, Fortier LA. Equine bone marrow-derived mesenchymal stromal cells are heterogeneous in MHC class II expression and capable of inciting an immune response in vitro.. Stem Cell Res Ther 2014 Jan 24;5(1):13.
    doi: 10.1186/scrt402pmc: PMC4055004pubmed: 24461709google scholar: lookup
  24. Barberini DJ, Freitas NP, Magnoni MS, Maia L, Listoni AJ, Heckler MC, Sudano MJ, Golim MA, da Cruz Landim-Alvarenga F, Amorim RM. Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential.. Stem Cell Res Ther 2014 Feb 21;5(1):25.
    doi: 10.1186/scrt414pmc: PMC4055040pubmed: 24559797google scholar: lookup
  25. Lew HL, Chen CP, Wang TG, Chew KT. Introduction to musculoskeletal diagnostic ultrasound: examination of the upper limb.. Am J Phys Med Rehabil 2007 Apr;86(4):310-21.
    doi: 10.1097/PHM.0b013e31803839acpubmed: 17413545google scholar: lookup
  26. Peetrons P. Ultrasound of muscles.. Eur Radiol 2002 Jan;12(1):35-43.
    doi: 10.1007/s00330-001-1164-6pubmed: 11868072google scholar: lookup
  27. Kol A, Wood JA, Carrade Holt DD, Gillette JA, Bohannon-Worsley LK, Puchalski SM, Walker NJ, Clark KC, Watson JL, Borjesson DL. Multiple intravenous injections of allogeneic equine mesenchymal stem cells do not induce a systemic inflammatory response but do alter lymphocyte subsets in healthy horses.. Stem Cell Res Ther 2015 Apr 15;6(1):73.
    doi: 10.1186/s13287-015-0050-0pmc: PMC4446064pubmed: 25888916google scholar: lookup
  28. Gala K, Burdzińska A, Idziak M, Wilczek E, Pączek L. Transplantation of mesenchymal stem cells into the skeletal muscle induces cytokine generation.. Cytokine 2013 Oct;64(1):243-50.
    doi: 10.1016/j.cyto.2013.06.314pubmed: 23859809google scholar: lookup

Citations

This article has been cited 1 times.
  1. MacDonald ES, Barrett JG. The Potential of Mesenchymal Stem Cells to Treat Systemic Inflammation in Horses. Front Vet Sci 2019;6:507.
    doi: 10.3389/fvets.2019.00507pubmed: 32039250google scholar: lookup
Subscribe to our newsletter
Join 99,357 horse owners like you who receive our email updates on horse health and nutrition.