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Home / Equine Research Bank / Sci Rep / Intravenous infusion of H2-saline suppresses oxidative stres...
Scientific reports2015; 5; 15514; doi: 10.1038/srep15514

Intravenous infusion of H2-saline suppresses oxidative stress and elevates antioxidant potential in Thoroughbred horses after racing exercise.

Abstract: Upon intensive, exhaustive exercise, exercise-induced reactive oxygen species may exceed the antioxidant defence threshold, consequently resulting in muscular damage or late-onset chronic inflammation. Recently, the therapeutic antioxidant and anti-inflammatory effects of molecular hydrogen (H2) for human rheumatoid arthritis have been demonstrated. However, it is also important to clarify the effects of administrating H2 in large animals other than humans, as H2 is thought to reach the target organ by passive diffusion upon delivery from the blood flow, indicating that the distance from the administration point to the target is critical. However, data on the effects of H2 on oxidative stress in real-life exhaustive exercise in large animals are currently lacking. We here investigated 13 Thoroughbred horses administered intravenous 2-L saline with or without 0.6-ppm H2 (placebo, N = 6; H2, N = 7) before participating in a high-intensity simulation race. Intravenous H2-saline significantly suppressed oxidative stress immediately, 3 h, and 24 h after the race, although the antioxidant capability was not affected throughout the study. The serum creatine kinase, lactate, and uric acid levels were increased in both groups. Taken together, these results indicate that intravenous H2-saline can significantly and specifically suppress oxidative stress induced after exhaustive racing in Thoroughbred horses.
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Publication Date: 2015-10-23 PubMed ID: 26493164PubMed Central: PMC4616033DOI: 10.1038/srep15514Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study explores how administering saline with molecular hydrogen (H2) before high-intensity exercise can successfully reduce the oxidative stress typically experienced by Thoroughbred horses after racing.

Overview of the Research

  • The study sets out to investigate the therapeutic effects of molecular hydrogen (H2) on the oxidative stress experienced by Thoroughbred horses post high-intensity exercise. Oxidative stress occurs when there is an imbalance between the production of free radicals and the ability of the body to counteract their harmful effects through neutralisation by antioxidants. This happens frequently in horses who partake in exhaustive exercise like racing, leading to issues like muscle damage or chronic inflammation.
  • The use of H2 as an antioxidant and anti-inflammatory agent has already been explored in humans, especially with conditions like rheumatoid arthritis. The researchers believe it’s important to verify if these effects translate to larger animals, considering the way H2 is thought to work — by passively diffusing from the bloodstream to the target organ. It’s hypothesized that the distance from the point of administration (in this case, intravenous) to the target organ might impact its effectiveness. However, there’s currently a lack of data on this aspect, hence the necessity for the study.

Methodology & Findings

  • The researchers conducted a high-intensity simulation race with 13 Thoroughbred horses. These horses were divided into two groups, with one group of 7 horses receiving an intravenous solution of 2-L saline with 0.6-ppm H2 before the race, while the remaining 6 horses served as the control group, receiving regular saline without H2.
  • The study found that the H2-saline group showed a significant suppression of oxidative stress immediately following the race, as well as at 3 hours and 24 hours after the exercise. However, there was no noticeable change in the antioxidant capability of the H2-treated horses, indicating that the H2-saline infusion did not enhance the natural capacity of the body to neutralize oxidative stress.
  • It was also observed that the levels of serum creatine kinase, lactate, and uric acid increased in both groups after the race — these are markers linked to muscle fatigue and damage, thus indicating that despite the H2-saline infusion, the exhaustive exercise still led to some level of muscle stress.
  • However, despite the muscle stress indicators, the key takeaway is that the intravenous administration of H2-saline effectively and specifically reduced the oxidative stress normally generated from high-intensity physical activity in Thoroughbred horses.

Cite This Article

APA
Yamazaki M, Kusano K, Ishibashi T, Kiuchi M, Koyama K. (2015). Intravenous infusion of H2-saline suppresses oxidative stress and elevates antioxidant potential in Thoroughbred horses after racing exercise. Sci Rep, 5, 15514. https://doi.org/10.1038/srep15514

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 5
Pages: 15514
PII: 15514

Researcher Affiliations

Yamazaki, Masahiko
  • Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-4-37 Takeda, Kofu, Yamanashi 400-8510, Japan.
  • Yamazaki Horse Clinic, 2-1-1 Mikoma, Miho-mura, Inasiki-gun, Ibaraki 300-0415, Japan.
Kusano, Kanichi
  • Japan Racing Association, Deputy Manager, Equine Department Veterinary Section, 11-1, Roppongi 6-chome Minato-ku, Tokyo 106-8401, Japan.
Ishibashi, Toru
  • Department of Rheumatology and Orthopaedic Surgery, Huis Ten Bosch Satellite H2 Clinic Hakata, 2-1 Gion, Hakata-ku, Fukuoka 812-0038, Japan.
  • Anicom Speciality Medical Institute, 1-5-22 Shimoochiai, Shinjuku-ku, Tokyo 161-0033, Japan.
Kiuchi, Masataka
  • Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 4-4-37 Takeda, Kofu, Yamanashi 400-8510, Japan.
Koyama, Katsuhiro
  • Graduate School Department of Interdisciplinary Research, University of Yamanashi 4-4-37 Takeda, Kofu, Yamanashi 400-8510, Japan.

MeSH Terms

  • Anaerobiosis
  • Animals
  • Antioxidants / pharmacology
  • Horses
  • Infusions, Intravenous
  • Oxidative Stress
  • Physical Conditioning, Animal
  • Sodium Chloride / chemistry

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