Invasive equine trophoblast expresses conventional class I major histocompatibility complex antigens.
Abstract: Monoclonal antibodies and alloantisera were used in an indirect immunohistochemical assay to determine the expression of class I and class II Major Histocompatibility Complex (MHC) antigens by equine placental cells and the endometrial tissues at the fetal-maternal interface. MHC class I antigens were expressed at high density on the surface of the trophoblast cells of the chorionic girdle at days 32-36, just prior to their invasion of the endometrium. The mature gonadotrophin-secreting cells of the endometrial cups, which are derived from the chorionic girdle cells, had greatly reduced levels of MHC class I antigen expression while no MHC class I antigens were detectable on the non-invasive trophoblast cells of the allantochorion, except in small isolated patches. MHC class I antigens immunoprecipitated from chorionic girdle cells with either monoclonal antibodies or alloantisera had a relative molecular mass of 44,000, which was identical to that of MHC class I antigens precipitated from lymphocytes with the same reagents. MHC class II antigens were not detected on any trophoblast cells, although they were expressed at high levels by the endometrial glandular and lumenal epithelium immediately bordering the endometrial cups. MHC class I antigens were also expressed at high levels by endometrial tissues in the area of the cups. The high level of MHC class I antigen expression by endometrial glands within and bordering the cups was in sharp contrast to the greatly reduced class I antigen expression by the mature endometrial cup cells themselves.(ABSTRACT TRUNCATED AT 250 WORDS)
Publication Date: 1990-09-01 PubMed ID: 2081471DOI: 10.1242/dev.110.1.63Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- Non-P.H.S.
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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This study explores the expression of class I and class II Major Histocompatibility Complex (MHC) antigens in horse placental cells and endometrial tissues. The results revealed a high level of MHC class I antigen expression in the trophoblast cells of the chorionic girdle before insemination of the endometrium.
Techniques Utilized
- The researchers used Monoclonal antibodies and alloantisera in an indirect immunohistochemical assay. This assay is used to test the expression of MHC antigens by tissue cells.
Key Findings
- MHC class I antigens were highly expressed on the surface of the trophoblast cells of the chorionic girdle during the days 32-36 before the embedding process of the endometrium.
- Progeny of the chorionic girdle cells, which are the mature gonadotrophin-secreting cells of the endometrial cups, showed reduced levels of MHC class I antigen expression.
- No detectable MHC class I antigens were found on the non-invasive trophoblast cells of the allantochorion, except in small isolated spaces.
- MHC class I antigens immunoprecipitated from chorionic girdle cells equaled the relative molecular mass of 44,000, matching that of MHC class I antigens precipitated from lymphocytes.
- No MHC class II antigens were detected on any trophoblast cells. These antigens were highly expressed by the endometrial glandular and lumenal epithelium around the endometrial cups.
- Despite the reduced class I antigen expression by the mature endometrial cup cells, the endometrial tissues in the cups area and those within and bordering the cups showed high expression of MHC class I antigens.
Implications of the Research
- This research aids in understanding the mechanisms involved in fetal-maternal interactions during pregnancy, more specifically in horses.
- The research provides insights into the behavior of MHC class I and II antigens in the placental cells and endometrial tissues impacting the fetal-maternal interface.
Cite This Article
APA
Donaldson WL, Zhang CH, Oriol JG, Antczak DF.
(1990).
Invasive equine trophoblast expresses conventional class I major histocompatibility complex antigens.
Development, 110(1), 63-71.
https://doi.org/10.1242/dev.110.1.63 Publication
Researcher Affiliations
- James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
MeSH Terms
- Animals
- Endometrium / immunology
- Female
- Fluorescent Antibody Technique
- Histocompatibility Antigens Class I / immunology
- Histocompatibility Antigens Class II / immunology
- Horses / immunology
- Immunohistochemistry
- Microscopy, Electron
- Microscopy, Electron, Scanning
- Pregnancy
- Pregnancy, Animal / immunology
- Trophoblasts / immunology
- Trophoblasts / ultrastructure
Grant Funding
- HD-15799 / NICHD NIH HHS
Citations
This article has been cited 11 times.- Skarzynski DJ, Bazer FW, Maldonado-Estrada JG. Editorial: Veterinary Reproductive Immunology.. Front Vet Sci 2021;8:823169.
- Rutigliano HM, Thomas AJ, Umbaugh JJ, Wilhelm A, Sessions BR, Kaundal R, Duhan N, Hicks BA, Schlafer DH, White KL, Davies CJ. Increased expression of pro-inflammatory cytokines at the fetal-maternal interface in bovine pregnancies produced by cloning.. Am J Reprod Immunol 2022 Mar;87(3):e13520.
- Antczak DF, Allen WRT. Placentation in Equids.. Adv Anat Embryol Cell Biol 2021;234:91-128.
- Jaworska J, Tobolski D, Janowski T. Is similarity in Major Histocompatibility Complex (MHC) associated with the incidence of retained fetal membranes in draft mares? A cross-sectional study.. PLoS One 2020;15(8):e0237765.
- Brosnahan MM, Silvela EJ, Crumb J, Miller DC, Erb HN, Antczak DF. Ectopic Trophoblast Allografts in the Horse Resist Destruction by Secondary Immune Responses.. Biol Reprod 2016 Dec;95(6):135.
- Buentjen I, Drews B, Frankenberg SR, Hildebrandt TB, Renfree MB, Menzies BR. Characterisation of major histocompatibility complex class I genes at the fetal-maternal interface of marsupials.. Immunogenetics 2015 Jul;67(7):385-93.
- Antczak DF, de Mestre AM, Wilsher S, Allen WR. The equine endometrial cup reaction: a fetomaternal signal of significance.. Annu Rev Anim Biosci 2013 Jan;1:419-42.
- Rapacz-Leonard A, Dąbrowska M, Janowski T. Major histocompatibility complex I mediates immunological tolerance of the trophoblast during pregnancy and may mediate rejection during parturition.. Mediators Inflamm 2014;2014:579279.
- de Mestre A, Noronha L, Wagner B, Antczak DF. Split immunological tolerance to trophoblast.. Int J Dev Biol 2010;54(2-3):445-55.
- Adams AP, Oriol JG, Campbell RE, Oppenheim YC, Allen WR, Antczak DF. The effect of skin allografting on the equine endometrial cup reaction.. Theriogenology 2007 Jul 15;68(2):237-47.
- Tallmadge RL, Lear TL, Antczak DF. Genomic characterization of MHC class I genes of the horse.. Immunogenetics 2005 Nov;57(10):763-74.
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