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Cytotherapy2013; 15(8); 1011-1020; doi: 10.1016/j.jcyt.2013.03.002

Investigating the efficacy of amnion-derived compared with bone marrow-derived mesenchymal stromal cells in equine tendon and ligament injuries.

Abstract: This is the first study to compare the treatment of horse tendon and ligament injuries with the use of mesenchymal stromal cells (MSCs) obtained from two different sources: amniotic membrane (AMSCs) and bone marrow (BM-MSCs). The objective was to prove the ability of AMSCs to exert beneficial effects in vivo. Methods: Five million allogeneic frozen-thawed AMSCs or autologous fresh BM-MSCs were injected intralesionally in horses belonging to group A (51 horses) and group B (44 horses). The interval lesion/implantation was of 6-15 days for the AMSCs and 16-35 days for the BM-MSCs. Healing was assessed clinically and ultrasonographically. Follow-up was monitored for 2 further years from return to full work. Results: No significant adverse effects after MSCs treatment were seen in any of the horses studied, independent of the type of stromal cell implanted. All animals belonging to group A resumed their activities between 4-5 months after treatment, whereas animals of group B resumed their activities after 4-12 months. The rate of re-injury in horses treated with AMSCs is lower (4.00%) compared with the average observed when horses were treated with BM-MSCs (23.08%). Conclusions: The possibility to inject allogeneic AMSCs in real time, before any ultrasonographic change occurs within the injured tendon and ligament, together with the higher plasticity and proliferative capacity of these cells compared with BM-MSCs, represents the main features of interest for this novel approach for the treatment of equine tendon diseases. An obvious active proliferative healing in the area injected with AMSCs makes these cells more effective than BM-MSCs.
Copyright © 2013 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.
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Publication Date: 2013-04-17 PubMed ID: 23602577DOI: 10.1016/j.jcyt.2013.03.002Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research aimed to compare the effectiveness of using mesenchymal stromal cells (MSCs) from two different sources, the amnion and bone marrow, in treating horse tendon and ligament injuries. The results indicated that treatment with amnion-derived MSCs is more effective than bone marrow-derived MSCs, with quicker recovery times and a lower rate of re-injury.

Research Methods

  • The researchers used two groups of horses to compare the treatment effects of amnion-derived mesenchymal stromal cells (AMSCs) and bone marrow-derived mesenchymal stromal cells (BM-MSCs).
  • Group A, consisting of 51 horses, received injections of AMSCs, while Group B, consisting of 44 horses, were treated with BM-MSCs.
  • The horses received treatment within specific timeframes after lesion occurrence, with the AMSC group receiving treatment between 6-15 days post-lesion and the BM-MSC group between 16-35 days post-lesion.
  • The researchers monitored the healing process of the horses clinically and ultrasonographically, observing the horses for an additional two years after they returned to full activity.

Results

  • The study found no significantly harmful effects from either type of MSC treatment.
  • All horses in Group A (that received AMSCs) resumed their activities within 4-5 months post-treatment, while those in Group B (treated with BM-MSCs) took notably longer, resuming activities 4-12 months post-treatment.
  • The rate of re-injury was also significantly lower in Group A (with only 4% experiencing re-injury) compared to Group B (with a re-injury rate of 23.08%).

Conclusions

  • The results of this study suggest that AMSCs may be more effective than BM-MSCs in the treatment of horse tendon and ligament injuries.
  • AMSCs can be injected in real-time (before ultrasonographic changes occur in the injured tendon or ligament), which may contribute to their increased effectiveness.
  • Additionally, the higher plasticity and proliferative capacity of AMSCs may make them a preferable treatment option compared to BM-MSCs, evident by the observable proliferative healing in the area injected with AMSCs.

Cite This Article

APA
Lange-Consiglio A, Tassan S, Corradetti B, Meucci A, Perego R, Bizzaro D, Cremonesi F. (2013). Investigating the efficacy of amnion-derived compared with bone marrow-derived mesenchymal stromal cells in equine tendon and ligament injuries. Cytotherapy, 15(8), 1011-1020. https://doi.org/10.1016/j.jcyt.2013.03.002

Publication

Cytotherapy
ISSN: 1477-2566
NlmUniqueID: 100895309
Country: England
Language: English
Volume: 15
Issue: 8
Pages: 1011-1020
PII: S1465-3249(13)00449-0

Researcher Affiliations

Lange-Consiglio, Anna
  • Università degli Studi di Milano, Large Animal Hospital, Reproduction Unit, Lodi, Italy.
Tassan, Stefano
    Corradetti, Bruna
      Meucci, Aurora
        Perego, Roberta
          Bizzaro, Davide
            Cremonesi, Fausto

              MeSH Terms

              • Amnion / cytology
              • Animals
              • Bone Marrow Cells / cytology
              • Cell Culture Techniques
              • Cell Differentiation
              • Cell- and Tissue-Based Therapy
              • Cells, Cultured
              • Female
              • Horses
              • Ligaments / injuries
              • Male
              • Mesenchymal Stem Cell Transplantation / veterinary
              • Mesenchymal Stem Cells / cytology
              • Tendon Injuries / therapy
              • Tendon Injuries / veterinary
              • Transplantation, Homologous / veterinary
              • Wound Healing

              Citations

              This article has been cited 27 times.
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