[Investigation of mare sera for antibodies against acholeplasmas and mycoplasmas with ther enzyme linked immunosorbent assay (ELISA) (author’s transl)].
Abstract: After abortion sera were taken from 58 thoroughbred and other mares of the northwestern part of Germany and investigated by ELISA (enzyme linked immuno-sorbent assay) for antibodies against Mycoplasma equirhinis, M. subdolum, M. equigenitalium, M. pulmonis, M. felis, Acholeplasma laidlawii, A. hippikon, and A. equifetale. Reactions at serum dilutions of 1:32 and higher were considered as positive. At serum dilution 1:32 no antibodies were found in 11 sera. The remaining sera showed antibodies against one or more of the mycoplasma antigens investigated. The number of multiple reactions decreased with an increasing dilution of the sera. Titers were found between 1:32 and 1:256. In one case a titer of 1:2048 against M. equigenitalium antigen was found. Most often antibodies against A. laidlawii were observed i.e. in 37 sera. These antibodies also showed the highest titers. Only 3 sera contained antibodies against A. hippikon. Antibodies against M. felis and A. equifetale were found in 26 sera. Between 10 and 15 sera showed antibodies against the remaining mycoplasma species.
Publication Date: 1980-01-01 PubMed ID: 7006267
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- English Abstract
- Journal Article
Summary
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The research article presents findings on the investigation of horse blood serum for antibodies against certain types of bacteria, using an enzyme linked immunosorbent assay (ELISA). The study was conducted on 58 mares in Germany who had recently miscarried.
Research Methodology
- The researchers collected blood serum from 58 mares from the northwestern part of Germany. These mares had recently aborted.
- The serum samples were investigated for the presence of antibodies against eight specific types of Mycoplasma and Acholeplasma bacteria using an ELISA test. This test uses enzyme reactions to identify and quantify substances, in this case, specific antibodies.
- When serums dilution of 1:32 and higher resulted in reactions, they were considered positive for these antibodies.
Results of the Study
- Eleven serum samples showed no antibodies when tested at a serum dilution of 1:32.
- The remaining serum samples showed the presence of antibodies against one or more of the eight types of bacteria being researched.
- The number of multiple reactions, where antibodies were found against more than one bacterial type, decreased as the dilution of the serum increased.
- Antibodies were found in varying concentrations, with titers between 1:32 and 1:256. In one instance, a titer of 1:2048 was found against M. equigenitalium bacteria.
Key Findings
- Antibodies against the bacterium A. laidlawii were most often observed. This was found in 37 serum samples and these antibodies also showed the highest titers.
- Only 3 sera contained antibodies against A. hippikon.
- Antibodies against M. felis and A. equifetale were found in 26 sera.
- Between 10 to 15 sera showed antibodies against the remaining mycoplasma species.
The results of this study indicate a potential prevalence of these bacteria in mares, especially of A. laidlawii bacterium, and may help in understanding the cause of miscarriages in these animals.
Cite This Article
APA
Ammar AM, Heitmann J, Kirchhoff H.
(1980).
[Investigation of mare sera for antibodies against acholeplasmas and mycoplasmas with ther enzyme linked immunosorbent assay (ELISA) (author’s transl)].
Zentralbl Bakteriol A, 247(4), 517-525.
Publication
Researcher Affiliations
MeSH Terms
- Abortion, Veterinary / immunology
- Acholeplasma / immunology
- Animals
- Antibodies, Bacterial / analysis
- Enzyme-Linked Immunosorbent Assay
- Female
- Horse Diseases / immunology
- Horses
- Immunoenzyme Techniques
- Mycoplasma / immunology
- Pregnancy
- Species Specificity
Citations
This article has been cited 4 times.- Beinborn M, Giebel J, Linck M, Cetin Y, Schwenk M, Sewing KF. Isolation, identification and quantitative evaluation of specific cell types from the mammalian gastric mucosa.. Cell Tissue Res 1993 Nov;274(2):229-40.
- Brown MB, Cassell GH, Taylor-Robinson D, Shepard MC. Measurement of antibody to Ureaplasma urealyticum by an enzyme-linked immunosorbent assay and detection of antibody responses in patients with nongonococcal urethritis.. J Clin Microbiol 1983 Feb;17(2):288-95.
- Gärtner K, Kirchhoff H, Mensing K, Velleuer R. The influence of social rank on the susceptibility of rats to Mycoplasma arthritidis.. J Behav Med 1989 Oct;12(5):487-502.
- Binder A, Gärtner K, Hedrich HJ, Hermanns W, Kirchhoff H, Wonigeit K. Strain differences in sensitivity of rats to Mycoplasma arthritidis ISR 1 infection are under multiple gene control.. Infect Immun 1990 Jun;58(6):1584-90.
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