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Home / Equine Research Bank / Int J Mol Sci / Investigation of MicroRNA Biomarkers in Equine Distal Interp...
International journal of molecular sciences2022; 23(24); 15526; doi: 10.3390/ijms232415526

Investigation of MicroRNA Biomarkers in Equine Distal Interphalangeal Joint Osteoarthritis.

Abstract: Osteoarthritis of the equine distal interphalangeal joint is a common cause of lameness. MicroRNAs from biofluids are promising biomarkers and therapeutic candidates. Synovial fluid samples from horses with mild and severe equine distal interphalangeal joint osteoarthritis were submitted for small RNA sequencing. The results demonstrated that miR-92a was downregulated in equine synovial fluid from horses with severe osteoarthritis and there was a significant increase in COMP, COL1A2, RUNX2 and SOX9 following miR-92a mimic treatment of equine chondrocytes in monolayer culture. This is the first equine study to evaluate the role of miR-92a in osteoarthritic chondrocytes in vitro.
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Publication Date: 2022-12-08 PubMed ID: 36555166PubMed Central: PMC9779011DOI: 10.3390/ijms232415526Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study examines potential indicators (microRNA biomarkers) for osteoarthritis in a specific joint in horses, also exploring a particular microRNA’s role in the disease.

Understanding the Context and Purpose of the Study

  • Osteoarthritis is a common cause of lameness in horses, particularly affecting the distal interphalangeal joint. This study investigates the potential role of microRNAs – small non-coding RNA molecules that regulate gene expression – as biomarkers for this disease.
  • The researchers specifically targeted synovial fluid (a joint lubricant) in horses with varying degrees of osteoarthritis for their study, using a process called small RNA sequencing to analyze its contents.

Key Findings of the Study

  • The results indicated that a specific microRNA, miR-92a, was less present (downregulated) in the synovial fluid from horses with severe osteoarthritis. This discovery highlights a potential correlation between the severity of osteoarthritis and miR-92a expression.
  • Furthermore, the research team found that treating equine chondrocytes (cells found in healthy cartilage) with a mimic of miR-92a resulted in a noticeable increase in the presence of COMP, COL1A2, RUNX2, and SOX9 – key molecules involved in cartilage formation and maintenance. This suggests that miR-92a has a role in these cells’ behavior and their response to osteoarthritis.

Significance of the Research

  • This study is unique as it is the first to explore the role of miR-92a in osteoarthritic chondrocytes of horses, providing more detail about the function of this molecule. It further expands the potential roles and impacts of microRNAs in animal and human osteoarthritis.
  • By identifying miR-92a as a possible biomarker for equine osteoarthritis, this study opens up new avenues for early detection and therapeutic intervention. Additional research is needed to confirm these results and explore potential clinical applications, such as detecting the disease before severe symptoms appear, or developing therapies that manipulate miR-92a activity to slow or halt disease progression.

Cite This Article

APA
Baker ME, Lee S, Clinton M, Hackl M, Castanheira C, Peffers MJ, Taylor SE. (2022). Investigation of MicroRNA Biomarkers in Equine Distal Interphalangeal Joint Osteoarthritis. Int J Mol Sci, 23(24), 15526. https://doi.org/10.3390/ijms232415526

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 23
Issue: 24
PII: 15526

Researcher Affiliations

Baker, Melissa E
  • The Roslin Institute, The University of Edinburgh, Easter Bush, Roslin EH25 9RG, UK.
Lee, Seungmee
  • The Roslin Institute, The University of Edinburgh, Easter Bush, Roslin EH25 9RG, UK.
Clinton, Michael
  • The RICE Group, Division of Gene Function and Development, Roslin Institute, The University of Edinburgh, Easter Bush, Roslin EH25 9RG, UK.
Hackl, Matthias
  • TAmiRNA GmbH, Leberstraße 20, 1110 Vienna, Austria.
Castanheira, Catarina
  • Department of Musculoskeletal and Ageing Science, Institute of Life Course and Medical Sciences, The University of Liverpool, William Henry Duncan Building, 6 West Derby Street, Liverpool L7 8TX, UK.
Peffers, Mandy J
  • Department of Musculoskeletal and Ageing Science, Institute of Life Course and Medical Sciences, The University of Liverpool, William Henry Duncan Building, 6 West Derby Street, Liverpool L7 8TX, UK.
Taylor, Sarah E
  • The Roslin Institute, The University of Edinburgh, Easter Bush, Roslin EH25 9RG, UK.

MeSH Terms

  • Horses
  • Animals
  • Osteoarthritis / genetics
  • Osteoarthritis / veterinary
  • Osteoarthritis / therapy
  • Joints
  • Synovial Fluid
  • Biomarkers

Grant Funding

  • Wellcome Trust

Conflict of Interest Statement

Matthias Hackl is an employee of TAmiRNA GmbH. No other author conflict of interest to declare.

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Citations

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