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Home / Equine Research Bank / Reproduction / Investigation of the stallion sperm proteome by mass spectro...
Reproduction (Cambridge, England)2014; 149(3); 235-244; doi: 10.1530/REP-14-0500

Investigation of the stallion sperm proteome by mass spectrometry.

Abstract: Stallion spermatozoa continue to present scientific and clinical challenges with regard to the biological mechanisms responsible for their survival and function. In particular, deeper understanding of sperm energy metabolism, defence against oxidative damage and cell-cell interactions should improve fertility assessment and the application of advanced reproductive technologies in the equine species. In this study, we used highly sensitive LC-MS/MS technology and sequence database analysis to identify and characterise the proteome of Percoll-isolated ejaculated equine spermatozoa, with the aim of furthering our understanding of this cell's complex biological machinery. We were able to identify 9883 peptides comprising 1030 proteins, which were subsequently attributed to 975 gene products. Gene ontology analysis for molecular and cellular processes revealed new information about the metabolism, antioxidant defences and receptors of stallion spermatozoa. Mitochondrial proteins and those involved in catabolic processes constituted dominant categories. Several enzymes specific to β-oxidation of fatty acids were identified, and further experiments were carried out to ascertain their functional significance. Inhibition of carnitine palmitoyl transferase 1, a rate-limiting enzyme of β-oxidation, reduced motility parameters, indicating that β-oxidation contributes to maintenance of motility in stallion spermatozoa.
© 2015 Society for Reproduction and Fertility.
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Publication Date: 2014-12-12 PubMed ID: 25504869DOI: 10.1530/REP-14-0500Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research is aimed at understanding the biological mechanisms underlying the survival and function of stallion spermatozoa, using proteomic analysis to reveal insights about its energy metabolism, defence against oxidative damage, and cell-cell interactions.

Objective of the Research

  • The research primarily focused on understanding the complex biological machinery of stallion spermatozoa. The goal was to enhance the understanding of energy metabolism, defence mechanisms against oxidative damage, and cellular interactions, with a potential outcome of improving fertility assessments and the application of advanced reproductive technologies in equine species.

Research Methodology

  • The study employed sensitive LC-MS/MS (Liquid Chromatography coupled with Tandem Mass Spectrometry) technology and sequence database analysis for characterising the proteome of Percoll-isolated ejaculated equine spermatozoa.
  • Gene ontology analysis was used to unravel the broader molecular and cellular processes.
  • Further experiments were also conducted to ascertain the functional significance of specific enzymes identified.

Research Findings

  • The proteomic analysis resulted in the identification of 9883 peptides and 1030 proteins, which are attributed to 975 gene products.
  • Insights into the metabolism, antioxidant defences, and receptors of stallion spermatozoa were gathered through gene ontology analysis.
  • It was discerned that mitochondrial proteins and those involved in catabolic processes were the dominant categories.
  • Several enzymes specific to β-oxidation of fatty acids were discovered. Subsequent experiments supported their functional significance in maintaining the motility of stallion spermatozoa.
  • The researchers noted that inhibition of carnitine palmitoyl transferase 1, a key enzyme of β-oxidation, reduced motility parameters.

Implication of Research

  • The research underscores the importance of β-oxidation in maintaining the motility of stallion spermatozoa. The inhibition of specific enzymes related to β-oxidation can potentially affect the sperm’s motility.
  • Moreover, the discovery and characterization of this cell’s proteome pave the way for an improved fertility assessment and better application of advanced reproductive technologies in the equine species.

Cite This Article

APA
Swegen A, Curry BJ, Gibb Z, Lambourne SR, Smith ND, Aitken RJ. (2014). Investigation of the stallion sperm proteome by mass spectrometry. Reproduction, 149(3), 235-244. https://doi.org/10.1530/REP-14-0500

Publication

Reproduction (Cambridge, England)
ISSN: 1741-7899
NlmUniqueID: 100966036
Country: England
Language: English
Volume: 149
Issue: 3
Pages: 235-244

Researcher Affiliations

Swegen, Aleona
  • Priority Research Centre for Reproductive ScienceSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, AustraliaAnalytical and Biomedical Research FacilityResearch Division, University of Newcastle, Callaghan, New South Wales, Australia aleona.swegen@uon.edu.au.
Curry, Benjamin J
  • Priority Research Centre for Reproductive ScienceSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, AustraliaAnalytical and Biomedical Research FacilityResearch Division, University of Newcastle, Callaghan, New South Wales, Australia.
Gibb, Zamira
  • Priority Research Centre for Reproductive ScienceSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, AustraliaAnalytical and Biomedical Research FacilityResearch Division, University of Newcastle, Callaghan, New South Wales, Australia.
Lambourne, Sarah R
  • Priority Research Centre for Reproductive ScienceSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, AustraliaAnalytical and Biomedical Research FacilityResearch Division, University of Newcastle, Callaghan, New South Wales, Australia.
Smith, Nathan D
  • Priority Research Centre for Reproductive ScienceSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, AustraliaAnalytical and Biomedical Research FacilityResearch Division, University of Newcastle, Callaghan, New South Wales, Australia.
Aitken, R John
  • Priority Research Centre for Reproductive ScienceSchool of Environmental and Life Sciences, University of Newcastle, Callaghan, New South Wales 2308, AustraliaAnalytical and Biomedical Research FacilityResearch Division, University of Newcastle, Callaghan, New South Wales, Australia.

MeSH Terms

  • Animals
  • Chromatography, Liquid
  • Horses / metabolism
  • Male
  • Proteome / analysis
  • Sperm Motility / physiology
  • Spermatozoa / metabolism
  • Tandem Mass Spectrometry

Citations

This article has been cited 24 times.
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