Isolation and characterization of a haemolysin from Trichophyton mentagrophytes.
Abstract: Haemolytic activities of Trichophyton (T.) mentagrophytes were detected and characterized by qualitative and quantitative assays. On Columbia agar supplemented with blood from horses, cattle or sheep, T. mentagrophytes expressed a strong zone of complete haemolysis. No haemolytic activities could be detected in the closely related T. verrucosum var. ochraceum. The same results were obtained after cultivation of the fungi on sterile cellulose acetate filters placed on the surface on Columbia blood agar. After removal of the filter, complete haemolysis was detected below the colony of T. mentagrophytes. A soluble haemolysin from culture supernatant of this strain was isolated and partially purified. Specific haemolytic activity per mg protein was enriched 2.6-fold in filtrate F(1), a fraction obtained as filtrate after filtration through 3kDa cut-off membranes. The partially purified haemolysin was neither affected by proteinase K treatment, nor by high and low temperatures, suggesting that it represents a small peptide haemolysin. Accordingly, in a commercial enzymatic activity test only the crude culture filtrate, but none of the subsequent purification fractions showed reactivity. Evaluation of the specificity of the haemolysin using erythrocytes from different mammalian species revealed that sensitivity was highest to those of equines, followed by erythrocytes from sheep, cattle, swine, dogs and humans. None of the erythrocytes was lysed by filtrate F(1) from T. verrucosum var. ochraceum. Furthermore, different eukaryotic cell lines from different species were tested in their sensitivity to cytolytic activities of the haemolysin, but no membrane damage could be detected.
Publication Date: 2007-02-03 PubMed ID: 17336469DOI: 10.1016/j.vetmic.2007.01.022Google Scholar: Lookup
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- Journal Article
Summary
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The article discusses the detection and characterization of haemolytic activities in the fungus Trichophyton mentagrophytes. The study also investigated the characteristics and effects of a soluble haemolysin from the fungus, noting its resistance to various external factors and its potency among various mammalian species.
Haemolytic Activities in T. mentagrophytes
- The T. mentagrophytes fungus displayed strong haemolytic activities – the ability to break down red blood cells.
- These activities were found using both qualitative and quantitative assays. Assays are scientific procedures performed to measure the presence or quantity of an analyte (in this case, haemolysin).
- When grown on Columbia agar supplemented with blood from horses, cattle, or sheep, T. mentagrophytes exhibited a significant zone of complete haemolysis, or complete breaking down of red blood cells.
- Interestingly, the closely-related T. verrucosum var. ochraceum did not show any haemolytic activities.
Isolation and Characterization of Haemolysin from T. mentagrophytes
- Researchers isolated and partially purified a soluble haemolysin from the culture supernatant of T. mentagrophytes.
- The haemolysin was found to be unaffected by the proteinase K treatment and by both high and low temperatures, implying that it is likely a small peptide haemolysin.
- Evaluation of the haemolysin’s specificity using red blood cells from different mammalian species showed that the haemolysin was most effective on equines (horses), followed by sheep, cattle, swine, dogs, and humans.
Lack of Haemolytic Activities in Other Strains and Cell Lines
- T. verrucosum var. ochraceum, a strain closely related to T. mentagrophytes, did not show any haemolytic activities.
- None of the red blood cells were broken down by the filtrate from T. verrucosum var. ochraceum, suggesting that this strain does not produce or release haemolysins in the same way that T. mentagrophytes does.
- Different eukaryotic cell lines from various species were tested for sensitivity to the cytolytic activities of the haemolysin, but researchers found no evidence of membrane damage.
Cite This Article
APA
Schaufuss P, Müller F, Valentin-Weigand P.
(2007).
Isolation and characterization of a haemolysin from Trichophyton mentagrophytes.
Vet Microbiol, 122(3-4), 342-349.
https://doi.org/10.1016/j.vetmic.2007.01.022 Publication
Researcher Affiliations
- Serumwerk Memsen WDT, Memsen 13, Hoyerhagen, Germany. schaufussdr@aol.com
MeSH Terms
- Animals
- Cattle
- Culture Media
- Dogs
- Electrophoresis, Polyacrylamide Gel
- Erythrocytes / microbiology
- Erythrocytes / physiology
- Hemolysin Proteins / isolation & purification
- Hemolysin Proteins / pharmacology
- Hemolysis / physiology
- Horses
- Humans
- Sensitivity and Specificity
- Sheep
- Species Specificity
- Swine
- Trichophyton / metabolism
- Trichophyton / pathogenicity
Citations
This article has been cited 4 times.- Kaveemongkonrat S, Duangsonk K, Houbraken J, Suwannaphong P, Vanittanakom N, Mekaprateep M. Partial characteristics of hemolytic factors secreted from airborne Aspergillus and Penicillium, and an enhancement of hemolysis by Aspergillus micronesiensis CAMP-like factor via Staphylococcus aureus-sphingomyelinase.. J Microbiol 2019 Dec;57(12):1086-1094.
- Alves IMS, Gonçalves VN, Oliveira FS, Schaefer CEGR, Rosa CA, Rosa LH. The diversity, distribution, and pathogenic potential of cultivable fungi present in rocks from the South Shetlands archipelago, Maritime Antarctica.. Extremophiles 2019 May;23(3):327-336.
- Pakshir K, Mohamadi T, Khodadadi H, Motamedifar M, Zomorodian K, Alipour S, Motamedi M. Proteolytic activity and cooperative hemolytic effect of dermatophytes with different species of bacteria.. Curr Med Mycol 2016 Dec;2(4):9-14.
- Gonçalves VN, Oliveira FS, Carvalho CR, Schaefer CEGR, Rosa CA, Rosa LH. Antarctic rocks from continental Antarctica as source of potential human opportunistic fungi.. Extremophiles 2017 Sep;21(5):851-860.
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