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Virology2012; 426(2); 93-99; doi: 10.1016/j.virol.2012.01.027

Isolation and characterization of a novel indigenous intestinal N4-related coliphage vB_EcoP_G7C.

Abstract: Lytic coliphage vB_EcoP_G7C and several other highly related isolates were obtained repeatedly from the samples of horse feces held in the same stable thus representing a component of the normal indigenous intestinal communities in this population of animals. The genome of G7C consists of 71,759 bp with terminal repeats of about 1160 bp, yielding approximately 73 kbp packed DNA size. Seventy-eight potential open reading frames, most of them unique to N4-like viruses, were identified and annotated. The overall layout of functional gene groups was close to that of the original N4 phage, with some important changes in late gene area including new tail fiber proteins containing hydrolytic domains. Structural proteome analysis confirmed all the predicted subunits of the viral particle. Unlike N4 itself, phage G7C did not exhibit a lysis-inhibited phenotype.
Copyright © 2012. Published by Elsevier Inc.
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Publication Date: 2012-02-15 PubMed ID: 22341309DOI: 10.1016/j.virol.2012.01.027Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the identification and analysis of a new bacteriophage (coliphage) found in horse feces, called vB_EcoP_G7C, which closely relates to the existing N4-like viruses.

Introduction and General Observations

  • The study involves isolating a new coliphage (bacteriophage that infects E.coli) named vB_EcoP_G7C and other related isolates from horse feces samples. Coliphages are commonly used as indicators of fecal contamination. In this case, they were consistently found in the feces from horses in one stable, suggesting that these bacteriophages form part of the regular intestinal communities in the horse population observed.

Genome Composition and Structure

  • The researchers sequenced the genome of the newly discovered coliphage G7C, revealing it to consist of 71,759 base pairs, with terminal repeats of about 1160 base pairs. With these terminal repeats, the total packed DNA size comes to approximately 73,000 base pairs.
  • They identified seventy-eight potential open reading frames within the genome. An open reading frame is a portion of the organism’s genome which contains the potential to be translated into a protein. Many of these potential open reading frames were unique to N4-like viruses, indicating that the isolated coliphage belongs to this group.

Similarity and Differences with Original N4 Phage

  • The organization of functional gene groups in the G7C coliphage is similar to that of the original N4 phage. However, there were notable changes in the late gene area, including new tail fiber proteins that encompass hydrolytic domains.
  • Structural proteome analysis confirmed the presence of all predicted subunits of the viral particle, i.e., all the proteins predicted to be part of the virus were detected.
  • Interestingly, unlike the original N4 able to inhibit lysis (the process by which it breaks down cells), the coliphage G7C exhibited no such lysis-inhibited characteristic.

Cite This Article

APA
Kulikov E, Kropinski AM, Golomidova A, Lingohr E, Govorun V, Serebryakova M, Prokhorov N, Letarova M, Manykin A, Strotskaya A, Letarov A. (2012). Isolation and characterization of a novel indigenous intestinal N4-related coliphage vB_EcoP_G7C. Virology, 426(2), 93-99. https://doi.org/10.1016/j.virol.2012.01.027

Publication

Virology
ISSN: 1096-0341
NlmUniqueID: 0110674
Country: United States
Language: English
Volume: 426
Issue: 2
Pages: 93-99

Researcher Affiliations

Kulikov, Eugene
  • Winogradsky Institute of Microbiology, Russian Academy of Science, pr. 60-letiya Oktyabrya 7, Building 2. 117 312, Moscow, Russia.
Kropinski, Andrew M
    Golomidova, Alla
      Lingohr, Erika
        Govorun, Vadim
          Serebryakova, Marina
            Prokhorov, Nikolai
              Letarova, Maria
                Manykin, Anatolij
                  Strotskaya, Alexandra
                    Letarov, Andrey

                      MeSH Terms

                      • Animals
                      • Bacteriophage N4 / classification
                      • Bacteriophage N4 / genetics
                      • Bacteriophage N4 / isolation & purification
                      • Coliphages / classification
                      • Coliphages / genetics
                      • Coliphages / isolation & purification
                      • Coliphages / physiology
                      • Feces / virology
                      • Genome, Viral
                      • Horses
                      • Host Specificity
                      • Intestines / virology
                      • Molecular Sequence Data
                      • Open Reading Frames

                      Citations

                      This article has been cited 27 times.
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