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Home / Equine Research Bank / Infect Immun / Isolation and characterization of an equine adenovirus.
Infection and immunity1973; 7(4); 673-677; doi: 10.1128/iai.7.4.673-677.1973

Isolation and characterization of an equine adenovirus.

Abstract: A viral agent was isolated from lung tissue obtained upon necropsy of an Arabian foal which had exhibited clinical signs of pneumonia. The virus is 75 nm in diameter, cubic in symmetry, and resistant to chloroform and low pH (3.0). It contains deoxyribonucleic acid and has a buoyant density of 1.31 g/cm(3) in cesium chloride. These findings indicate that the virus is a member of the adenovirus group.
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Publication Date: 1973-04-01 PubMed ID: 16558078PubMed Central: PMC422741DOI: 10.1128/iai.7.4.673-677.1973Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about a study where an adenovirus was successfully isolated from lung tissue of a sick Arabian foal. The virus exhibited traits commonly associated with adenoviruses and contributed to the development of pneumonia in the animal.

Virus Isolation and Identification

  • The study was initiated following the death of an Arabian foal that showed symptoms of pneumonia. During the post-death examination, commonly known as necropsy, lung tissue was obtained for further investigation.
  • From this lung tissue, researchers successfully isolated a viral agent that was causing the identified symptoms.

Characteristics of the Isolated Virus

  • The isolated virus measures 75 nm in diameter and has a cubic in symmetry. Its cubic symmetry suggests its arrangement of structural units, and the size is relatively consistent with known adenoviruses which commonly range between 70-90 nm in diameter.
  • It is resistant to chloroform, a chemical usually used to disrupt and kill viruses by dissolving the viral envelope. This resistance is typical to adenoviruses due to the absence of a lipid envelope.
  • The virus is also resistant to a low pH (3.0) environment. Adenoviruses are known to be stable in highly acidic conditions and can survive in a wide range of pH levels.
  • The virus is trackable as it contains DNA. A DNA virus presents a potential advantage over RNA viruses in the field of virology because DNA is less prone to mutations during replication, hence ensuring stability of the identified virus.
  • The discovered virus has a buoyant density of 1.31 g/cm(3) in cesium chloride. This implies that when placed in cesium chloride, a medium used in a technique known as density gradient centrifugation, it settles at a level where the density is 1.31 g/cm(3). This density is characteristic of adenoviruses.

Conclusion of Findings

  • Based on the characteristics such as size, symmetry, resistance to chloroform and low pH, DNA composition, and density in cesium chloride, it was concluded that the isolated virus from the Arabian foal is a member of the adenovirus group.

Cite This Article

APA
Ardans AA, Pritchett RF, Zee YC. (1973). Isolation and characterization of an equine adenovirus. Infect Immun, 7(4), 673-677. https://doi.org/10.1128/iai.7.4.673-677.1973

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 7
Issue: 4
Pages: 673-677

Researcher Affiliations

Ardans, A A
  • Departments of Clinical Sciences and Microbiology, School of Veterinary Medicine, University of California, Davis, California 95616.
Pritchett, R F
    Zee, Y C

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      Citations

      This article has been cited 1 times.
      1. Clayton FW. Combined (B- and T-lymphocyte) immunodeficiency in an Arabian foal.. Can Vet J 1976 Jan;17(1):26-8.
        pubmed: 1083284
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