Isolation and characterization of β-haemolytic-Streptococci from endometritis in mares.
Abstract: The objective of this manuscript was to validate published PCR-based methods for detection of β-haemolytic Streptococci by comparison with established bacteriological techniques using 85 clinical isolates recovered from uterine swabs of mares with clinical signs of endometritis and to determine the distribution of SeeL/SeeM and SzeL/SzeM superantigens in isolates of Streptococcus equi subsp. equi (S. equi) and S. equi subsp. zooepidemicus (S. zooepidemicus). The conventional bacteriological techniques showed the vast majority of these isolates (78) were S. zooepidemicus with just 5 Streptococcus dysgalactiae subsp. equisimilis (S. equisimilis) and 2 S. equi strains detected. The PCR analyses confirmed the bacteriological results demonstrating the reliability of the 16S rRNA PCR assay for detecting Streptococci, the multiplex PCR for differentiating between S. zooepidemicus, and S. equi, and PCR assays based on streptokinase genes for identification of S. equisimilis. PCRs for genes encoding superantigens revealed seeL and seeM specific amplicons with size of approximately 800 and 810 bp respectively for the S. equi strains and for 2 S. zooepidemicus strains. To our knowledge, this is the first report of szeL and szeM possession by S. zooepidemicus isolates derived from endometritis in mares.
Copyright © 2011 Elsevier B.V. All rights reserved.
Publication Date: 2011-04-16 PubMed ID: 21570219DOI: 10.1016/j.vetmic.2011.04.009Google Scholar: Lookup
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- Journal Article
- Validation Study
Summary
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This research sought to confirm the reliability of using PCR-based methods for detecting β-haemolytic Streptococci in horses suffering from endometritis, compared to conventional bacteriological tests. The study also aimed to identify the distribution of specific superantigens within isolated Streptococcus strains.
Objective of the Research
- This study aimed to validate the use of Polymerase chain reaction (PCR) techniques for identifying β-haemolytic Streptococci bacteria—a common cause of endometritis in mares.
- Researchers wanted to compare PCR results with traditional bacteriological tests, and likewise, identify the distribution and presence of specific superantigens in certain Streptococcus strains.
Methodology and Findings
- For this purpose, 85 clinical isolates recovered from uterine swabs of horses displaying endometritis symptoms were tested.
- Conventional testing methods revealed the majority of these isolates were Streptococcus equi subsp. zooepidemicus (S. zooepidemicus), with some S. dysgalactiae subsp. equisimilis and S. equi being detected.
- PCR analyses confirmed the reliability of 16S rRNA PCR assay for detecting Streptococci, in addition to differentiating S. zooepidemicus and S. equi.
- Researchers also introduced PCR assays based on streptokinase genes for identifying S. equisimilis.
Significance of the Research
- The researchers were able to validate the use of PCR techniques over traditional testing methods, hence this study bridges the gap in distinguishing different types of Streptococci related to endometritis in mares using molecular techniques.
- This study also spotted the possession of specific superantigens by S. zooepidemicus isolates derived from endometritis in horses for the first time. The superantigens SeeL/SeeM and SzeL/SzeM were found in isolates of S. equi strains and S. zooepidemicus strains, a finding that might influence the development of new therapeutic measures against these bacteria.
Cite This Article
APA
Casagrande Proietti P, Bietta A, Coppola G, Felicetti M, Cook RF, Coletti M, Marenzoni ML, Passamonti F.
(2011).
Isolation and characterization of β-haemolytic-Streptococci from endometritis in mares.
Vet Microbiol, 152(1-2), 126-130.
https://doi.org/10.1016/j.vetmic.2011.04.009 Publication
Researcher Affiliations
- Department of Biopathological Science and Hygiene of Food and Animal Production, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy. patrizia.casagrandeproietti@unipg.it
MeSH Terms
- Animals
- DNA, Bacterial / genetics
- Endometritis / microbiology
- Female
- Horses / microbiology
- Polymerase Chain Reaction / methods
- Polymerase Chain Reaction / veterinary
- Reproducibility of Results
- Streptococcal Infections / veterinary
- Streptococcus / genetics
- Streptococcus / isolation & purification
- Superantigens / genetics
Citations
This article has been cited 4 times.- Garner C, Stephen C, Pant SD, Ghorashi SA. Comparison of PCR-HRM, colorimetric LAMP and culture based diagnostic assays in the detection of endometritis caused by Streptococcus equi subsp. zooepidemicus in mares. Vet Res Commun 2023 Jun;47(2):495-509.
- Zhao Y, Zhu Y, Liu B, Mi J, Li N, Zhao W, Wu R, Holyoak GR, Li J, Liu D, Zeng S, Wang Y. Antimicrobial Susceptibility of Bacterial Isolates from Donkey Uterine Infections, 2018-2021. Vet Sci 2022 Feb 5;9(2).
- Kinoshita Y, Niwa H, Katayama Y. Development of a loop-mediated isothermal amplification method for detecting Streptococcus equi subsp. zooepidemicus and analysis of its use with three simple methods of extracting DNA from equine respiratory tract specimens. J Vet Med Sci 2014 Sep;76(9):1271-5.
- Haenni M, Lupo A, Madec JY. Antimicrobial Resistance in Streptococcus spp. Microbiol Spectr 2018 Mar;6(2).
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