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Home / Equine Research Bank / In Vitro Cell Dev Biol Anim / Isolation and differentiation of endometrial mesenchymal ste...
In vitro cellular & developmental biology. Animal2025; 61(10); 1187-1192; doi: 10.1007/s11626-025-01103-5

Isolation and differentiation of endometrial mesenchymal stem cells from Arabian mares.

Abstract: This study aimed to extract and isolate endometrial stromal cells from Arabian mares and investigate their growth and differentiation potential. Endometrial biopsies were obtained from three healthy 6-year-old Arabian mares using a standardized, minimally invasive protocol. The isolated cells were characterized using flow cytometry and differentiation analysis. Flow cytometry revealed mesenchymal markers CD90 (95.2%) and CD105 (97.4%) and hematopoietic markers CD34 (1.17%) and CD45 (0.339%). The cells exhibited differentiation potential into adipocytes, osteoblasts, and chondrocytes. The findings suggest that endometrial cells from Arabian mares represent a promising autologous source of MSCs, particularly suited for regenerative applications in musculoskeletal disorders.
© 2025. The Society for In Vitro Biology.
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Publication Date: 2025-11-25 PubMed ID: 41288937PubMed Central: 9311888DOI: 10.1007/s11626-025-01103-5Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

Overview

  • This research focuses on isolating stem cells from the endometrial tissue of Arabian mares and studying their ability to grow and transform into different cell types.

Research Objectives

  • To extract and isolate endometrial stromal cells from Arabian mares.
  • To investigate the growth characteristics and differentiation potential of these isolated cells.

Methodology

  • Endometrial biopsies were collected from three healthy 6-year-old Arabian mares.
  • The biopsy procedure employed a standardized, minimally invasive approach to ensure animal welfare and sample consistency.
  • Cells were isolated from the tissue samples for subsequent analysis.

Cell Characterization Techniques

  • Flow cytometry was used to identify the type of cells based on surface markers.
  • Markers tested included mesenchymal stem cell (MSC) markers CD90 and CD105, as well as hematopoietic (blood cell) markers CD34 and CD45.
  • High expression of CD90 (95.2%) and CD105 (97.4%) indicated a strong presence of mesenchymal stem cells.
  • Low expression of CD34 (1.17%) and CD45 (0.339%) confirmed minimal hematopoietic contamination.

Differentiation Potential

  • Isolated endometrial stromal cells were tested for their ability to differentiate into three specialized cell types:
    • Adipocytes (fat cells)
    • Osteoblasts (bone-forming cells)
    • Chondrocytes (cartilage cells)
  • Successful differentiation indicated that these cells behave like mesenchymal stem cells capable of multilineage differentiation.

Significance and Applications

  • The study suggests that endometrial tissue from Arabian mares is a viable and promising source of autologous mesenchymal stem cells (MSCs).
  • This is particularly relevant for regenerative medicine, such as treatments of musculoskeletal disorders in horses.
  • Using autologous (self-derived) cells reduces the risk of immune rejection and enhances treatment safety and efficacy.
  • Since the isolation method is minimally invasive, it is practical and less harmful for repeated sampling.

Summary

  • The research successfully isolated and characterized mesenchymal stem cells from the endometrium of Arabian mares.
  • These cells exhibit hallmark MSC markers and can differentiate into fat, bone, and cartilage cells.
  • Such cells represent a promising resource for autologous regenerative therapies, especially in veterinary musculoskeletal medicine.

Cite This Article

APA
Sabzpoosh M, Hoveizi E, Gooraninejad S. (2025). Isolation and differentiation of endometrial mesenchymal stem cells from Arabian mares. In Vitro Cell Dev Biol Anim, 61(10), 1187-1192. https://doi.org/10.1007/s11626-025-01103-5

Publication

In vitro cellular & developmental biology. Animal
ISSN: 1543-706X
NlmUniqueID: 9418515
Country: Germany
Language: English
Volume: 61
Issue: 10
Pages: 1187-1192

Researcher Affiliations

Sabzpoosh, Mohammadreza
  • Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Hoveizi, Elham
  • Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran. e.hoveizi@scu.ac.ir.
Gooraninejad, Saad
  • Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

MeSH Terms

  • Animals
  • Female
  • Horses
  • Mesenchymal Stem Cells / cytology
  • Cell Differentiation
  • Endometrium / cytology
  • Cell Separation / methods
  • Flow Cytometry
  • Adipocytes / cytology
  • Osteoblasts / cytology

Grant Funding

  • 1401 / Shahid Chamran University of Ahvaz

Conflict of Interest Statement

Declarations. Ethics approval: All animal protocols were approved by and performed in accordance with the Institutional Animal Ethics Committee of the Shahid Chamran University of Ahvaz (protocol number EE/1401.2.24.204108/scu.ac.ir). Also, all protocols complied with ARRIVE guidelines, and all the procedures strictly followed the Animal Scientific Procedures Act (1986). Competing interests: The authors declare no competing interests.

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