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Home/Equine Research Bank/Stem Cells Dev/The Fate of Autologous Endometrial Mesenchymal Stromal Cells...
Stem cells and development2018; 27(15); 1046-1052; doi: 10.1089/scd.2018.0056

The Fate of Autologous Endometrial Mesenchymal Stromal Cells After Application in the Healthy Equine Uterus.

Abstract: Because of their distinct differentiation, immunomodulatory, and migratory capacities, endometrial mesenchymal stromal cells (MSCs) may provide an optimum source of therapeutic cells not only in relation to the uterus but also for regeneration of other tissues. This study reports the fate of endometrial MSCs following intrauterine application in mares. Stromal cell fractions were isolated from endometrial biopsies taken from seven reproductively healthy mares, expanded, and fluorescence labeled in culture. Phosphate-buffered saline (PBS) or MSCs (15 × 10) were autologously infused into each uterine horn during early diestrus and subsequently tracked by fluorescence microscopy and flow cytometry of endometrial biopsies and blood samples taken periodically after infusion. The inflammatory response to cell infusion was monitored in endometrial cytology samples. MSCs were detected in endometrial sections at 6, 12, and 24 h, but not later (7 or 14 days), after cell infusion. Cells were in all cases located in the uterine lumen, never within the endometrial tissue. No fluorescence signal was detected in blood samples at any time point after infusion. Cytology analyses showed an increase in % of polymorphonuclear neutrophils between 1 and 3 h after uterine infusion with either MSCs or PBS and a further increase by 6 h only in mares infused with PBS. In summary, endometrial MSCs were detected in the uterine lumen for up to 24 h after infusion, but did not migrate into the healthy endometrium. Moreover, MSCs effectively attenuated the inflammatory response to uterine infusion. We conclude that endometrial MSCs obtained from routine uterine biopsies could provide a safe and effective cell source for treatment of inflammatory conditions of the uterus and potentially other tissues.
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Publication Date: 2018-06-29 PubMed ID: 29790424PubMed Central: PMC6067096DOI: 10.1089/scd.2018.0056Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the behavior and effects of endometrial mesenchymal stromal cells (MSCs), found in the uterus of mares, after they are reintroduced to the uterus. The researchers note that these MSCs don’t migrate into the healthy endometrium tissues, but stay in the uterine lumen up to 24 hours after the infusion, and have the potential to decrease the inflammatory response in the uterus.

Research Methodology

  • Stromal cell fractions were isolated from biopsies of the endometrial tissue taken from seven reproductively healthy mares.
  • These cells were grown and labeled with fluorescence in a culture.
  • A solution of either phosphate-buffered saline (PBS) or MSCs were reintroduced into each uterine horn during early diestrus, a phase of the mare’s reproductive cycle.
  • Using fluorescence microscopy and flow cytometry, the cells were tracked in biopsies and blood samples taken at various periods after infusion.
  • The inflammatory response due to the cell infusion was monitored using endometrial cytology samples.

Key Findings

  • The MSCs could be detected within the uterine lumen at 6, 12, and 24 hours after infusion. However, they were not detected later, at 7 or 14 days, after the cell infusion.
  • These cells were always found in the uterine lumen, and never migrated into the endometrial tissue.
  • There was no fluorescence signal from the cells found in the blood samples at any point after infusion.
  • The cytology analysis showed that there was an increase in neutrophils, immune cells that respond to inflammation, between 1 and 3 hours after the uterus was infused with either MSCs or PBS. However, there was a further increase by 6 hours only in mares infused with PBS.

Research Conclusions

  • The researchers concluded that endometrial MSCs stay in the uterine lumen for approximately 24 hours but do not migrate into healthy endometrial tissues.
  • These cells effectively soothe the inflammatory response to the infusion process in the uterus.
  • Based on their results, they suggest that MSCs obtained from regular uterine biopsies could be a safe and effective solution for treating inflammatory conditions in the uterus and potentially in other tissues.

Cite This Article

APA
Rink BE, Beyer T, French HM, Watson E, Aurich C, Donadeu FX. (2018). The Fate of Autologous Endometrial Mesenchymal Stromal Cells After Application in the Healthy Equine Uterus. Stem Cells Dev, 27(15), 1046-1052. https://doi.org/10.1089/scd.2018.0056

Publication

Stem cells and development
ISSN: 1557-8534
NlmUniqueID: 101197107
Country: United States
Language: English
Volume: 27
Issue: 15
Pages: 1046-1052

Researcher Affiliations

Rink, B Elisabeth
  • 1 Department of Clinical Sciences, Ross University School of Veterinary Medicine , St.Kitts, West Indies .
  • 2 The Roslin Institute, University of Edinburgh , Edinburgh, United Kingdom .
  • 3 Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine , Vienna, Austria .
Beyer, Teresa
  • 3 Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine , Vienna, Austria .
French, Hilari M
  • 1 Department of Clinical Sciences, Ross University School of Veterinary Medicine , St.Kitts, West Indies .
Watson, Elaine
  • 1 Department of Clinical Sciences, Ross University School of Veterinary Medicine , St.Kitts, West Indies .
Aurich, Christine
  • 3 Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, University of Veterinary Medicine , Vienna, Austria .
Donadeu, F Xavier
  • 2 The Roslin Institute, University of Edinburgh , Edinburgh, United Kingdom .

MeSH Terms

  • Animals
  • Cell Differentiation / genetics
  • Cell Movement / genetics
  • Endometrium / growth & development
  • Endometrium / transplantation
  • Female
  • Horses
  • Mesenchymal Stem Cell Transplantation
  • Mesenchymal Stem Cells / cytology
  • Neutrophils / metabolism
  • Regeneration / genetics
  • Uterus / cytology
  • Uterus / growth & development

Conflict of Interest Statement

No competing financial interests exist.

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Citations

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