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Endometrial inflammatory markers of the early immune response in mares susceptible or resistant to persistent breeding-induced endometritis.
Abstract: Transient endometritis after breeding is necessary for clearance of bacteria and spermatozoa; however, in a subpopulation of mares, the inflammation fails to resolve in a timely fashion. The objective of this study was to describe the uterine inflammatory response in mares susceptible or resistant to persistent breeding-induced endometritis (PBIE) during the first 24 h after induction of uterine inflammation.Twelve mares were classified as susceptible (nZ6) or resistant (nZ6) to PBIE. Mares were inseminated over five estrous cycles and endometrial biopsies were collected at one time point per cycle before (0) and 2, 6, 12, and 24 h after insemination. qPCR analysis for IL1B, IL6, IL8, IFNG, TNF (TNFA), IL10, and IL1RN was performed, and endometrial inflammatory cells were counted for each sample. Relative quantification values reported fold changes in mRNA expression from 0 h values. A general pattern of expression post insemination was observed in both groups of mares. Cytokine mRNA increased at 2 h, peaked between 2 and 12 h, and then decreased.Differences were detected between groups of mares 6 h after challenge; resistant mares had higher mRNA expression of IL6, IL1RN,and IL10 than susceptible mares. Susceptible mares had an increased number of polymorphonuclear neutrophils in the endometrium 2 and 12 h after breeding when compared with resistant mares. These findings describe an inherent difference in the initial immune response to insemination and may help explain the transient nature of inflammation in resistant mares, whereas susceptible mares develop a persistent inflammation.
Publication Date: 2013-04-13 PubMed ID: 23580950DOI: 10.1530/rep-12-0452Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article focused on investigating the early uterine inflammatory responses in mares that are susceptible or resistant to persistent breeding-induced endometritis, finding that resistant mares had higher expression of certain immune response markers than susceptible mares.
Objective and Methodology
- The primary objective of the study was to analyze the uterine inflammatory response in mares susceptible or resistant to persistent breeding-induced endometritis (PBIE) within the first 24 hours after provoking uterine inflammation.
- The method involved classifying twelve mares as either susceptible or resistant to PBIE. The mares were inseminated over five estrous cycles and endometrial biopsies were taken at different time points—before and after insemination.
- The biopsied samples underwent qPCR analysis to identify changes in the expression of various inflammatory indicators: IL1B, IL6, IL8, IFNG, TNF (TNFA), IL10, and IL1RN. Endometrial inflammatory cells in each sample were also counted.
Findings
- The results showed a common pattern of expression post-insemination in both groups of mares. The cytokine mRNA levels increased at 2 hours, peaked between 2 and 12 hours, and then decreased after peaks.
- Significant differences were noted in the mare groups 6 hours post-challenge. Resistant mares exhibited higher mRNA expression of IL6, IL1RN, and IL10 than susceptible mares. This suggests that the immune response to insemination varied between the resistant and susceptible mares.
- Susceptible mares had an increased number of polymorphonuclear neutrophils (a type of white blood cell usually associated with inflammation) in the endometrium 2 and 12 hours after breeding than the resistant mares.
Implications
- The study’s findings describe an inherent difference in the initial immune response to insemination in the investigated mares. This variation in immune response may explain why resistant mares only have transient inflammation while susceptible mares suffer persistent inflammation.
- This research might aid in understanding the biological mechanisms behind the development of persistent endometritis in mares, providing potential avenues for preventive or therapeutic treatment.
Cite This Article
APA
Woodward EM, Christoffersen M, Campos J, Betancourt A, Horohov D, Scoggin KE, Squires EL, Troedsson MH.
(2013).
Endometrial inflammatory markers of the early immune response in mares susceptible or resistant to persistent breeding-induced endometritis.
Reproduction, 145(3), 289-296.
https://doi.org/10.1530/rep-12-0452 Publication
Researcher Affiliations
- Department of Veterinary Science, The Maxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546-0099, USA. elizabeth.woodward@uky.edu
MeSH Terms
- Animals
- Biopsy / veterinary
- Breeding
- Cytokines / genetics
- Cytokines / metabolism
- Disease Susceptibility
- Endometriosis / genetics
- Endometriosis / immunology
- Endometriosis / pathology
- Endometriosis / veterinary
- Endometrium / immunology
- Endometrium / pathology
- Female
- Gene Expression Regulation
- Horse Diseases / genetics
- Horse Diseases / immunology
- Horse Diseases / pathology
- Horses
- Inflammation Mediators / metabolism
- Insemination, Artificial / adverse effects
- Insemination, Artificial / veterinary
- RNA, Messenger / metabolism
- Real-Time Polymerase Chain Reaction / veterinary
- Reverse Transcriptase Polymerase Chain Reaction / veterinary
- Risk Factors
- Time Factors
Citations
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