Isolation and identification of Taylorella asinigenitalis from the genital tract of a stallion, first case of a natural infection.
Abstract: Contagious equine metritis (CEM), caused by Taylorella equigenitalis, is a widely known highly contagious genital equine disease that is transmitted venereally. A new bacterium, Taylorella asinigenitalis resembling T. equigenitalis was recently isolated from three American donkey jacks, at routine testing for CEM. The purpose of this study was to identify and characterize a strain of Taylorella sp. from the genital tract of a stallion. Swab samples for culture of T. equigenitalis were taken from urethral fossa, urethra and penile sheath of a 3-year-old stallion of the Ardennes breed when it was routinely tested for CEM. A small Gram-negative rod was isolated, but the colony appearance, the slow growth rate and the results in the API ZYM test differed slightly from those of T. equigenitalis. Sequencing of the 16S rRNA gene was therefore performed and phylogenetic analysis demonstrated that the sequence of the strain Bd 3751/05 represents T. asinigenitalis and that the strain is identical with the Californian asinine strain UCD-1T (ATCC 700933T). The T. asinigenitalis strain had a low MIC of gentamicin (MIC16 microg/ml). Taylorella asinigenitalis has thus for the first time been isolated from the genital tract of a stallion with a natural infection. To determine the pathogenicity of T. asinigenitalis it will be important to conduct further experimental studies. Sequence analysis of 16S rRNA genes was shown to be a reliable tool for differentiation of T. asinigenitalis from T. equigenitalis as well as for identification of these species.
Publication Date: 2006-05-07 PubMed ID: 16793226DOI: 10.1016/j.vetmic.2006.04.027Google Scholar: Lookup
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Summary
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This study provides details to the first-ever recorded natural infection in a stallion’s genital tract caused by the bacterium Taylorella asinigenitalis. The researchers further emphasised the need for additional studies to understand the pathogenicity of T. asinigenitalis, validating the use of sequence analysis of 16S rRNA genes as a reliable tool in identifying and differentiating T. asinigenitalis from T. equigenitalis.
Purpose of the Study
- The purpose of this research was to identify and characterize a strain of Taylorella sp., taken from the genital tract of a stallion. This was following the recent discovery of a new bacterium, T. asinigenitalis, in American donkey jacks.
Methodology
- Swab samples for culture of T. equigenitalis were taken from various areas of a 3-year-old Ardennes breed stallion’s genital tract undergoing routine testing for Contagious equine metritis (CEM).
- A small Gram-negative rod was isolated from these swab samples. The researchers noted differences in the colony appearance, growth rate, and results in the API ZYM test, indicating this was not T. equigenitalis.
- To accurately identify the strain, sequencing of the 16S rRNA gene was performed.
Results and Findings
- Phylogenetic analysis demonstrated that the gene sequence of the isolated strain was identical to T. asinigenitalis found in a Californian asinine.
- The study recorded and identified T. asinigenitalis as the cause for a natural infection within a stallion’s genital tract for the first time ever.
- T. asinigenitalis demonstrated a low MIC of gentamicin, but a high MIC of streptomycin.
Implications and future directions
- The results of the study highlight the importance of conducting further experimental studies to understand the pathogenicity of T. asinigenitalis.
- It confirms that sequence analysis of 16S rRNA genes is an effective tool for differentiating T. asinigenitalis from T. equigenitalis and for identifying these species.
Cite This Article
APA
Båverud V, Nyström C, Johansson KE.
(2006).
Isolation and identification of Taylorella asinigenitalis from the genital tract of a stallion, first case of a natural infection.
Vet Microbiol, 116(4), 294-300.
https://doi.org/10.1016/j.vetmic.2006.04.027 Publication
Researcher Affiliations
- Department of Bacteriology, National Veterinary Institute, SE-751 89 Uppsala, Sweden. viveca.baverud@sva.se
MeSH Terms
- Animals
- Anti-Bacterial Agents / pharmacology
- Anti-Bacterial Agents / therapeutic use
- Base Sequence
- DNA, Ribosomal / chemistry
- Diagnosis, Differential
- Gram-Negative Bacterial Infections / diagnosis
- Gram-Negative Bacterial Infections / drug therapy
- Gram-Negative Bacterial Infections / microbiology
- Gram-Negative Bacterial Infections / veterinary
- Horse Diseases / diagnosis
- Horse Diseases / drug therapy
- Horse Diseases / microbiology
- Horses
- Male
- Microbial Sensitivity Tests / veterinary
- Molecular Sequence Data
- Phylogeny
- RNA, Bacterial / genetics
- RNA, Ribosomal, 16S / genetics
- Species Specificity
- Taylorella / classification
- Taylorella / drug effects
- Taylorella / genetics
- Taylorella / isolation & purification
- Taylorella equigenitalis / classification
- Taylorella equigenitalis / drug effects
- Taylorella equigenitalis / isolation & purification
Citations
This article has been cited 7 times.- Wang Z, Zeng S, Wang Y, Wang T, Qu H, Zhu Y, Li J. Reproductive Disorders in Donkeys: Current Evidence and Update. Animals (Basel) 2024 Aug 28;14(17).
- Tyrnenopoulou P, Fthenakis GC. Clinical Aspects of Bacterial Distribution and Antibiotic Resistance in the Reproductive System of Equids. Antibiotics (Basel) 2023 Mar 28;12(4).
- Nadin-Davis S, Knowles MK, Burke T, Böse R, Devenish J. Comparison of culture versus quantitative real-time polymerase chain reaction for the detection of Taylorella equigenitalis in field samples from naturally infected horses in Canada and Germany. Can J Vet Res 2015 Jul;79(3):161-9.
- Hara Y, Hayashi K, Nakajima T, Kagawa S, Tazumi A, Moore JE, Matsuda M. Molecular identification and characterization of clustered regularly interspaced short palindromic repeat (CRISPR) gene cluster in Taylorella equigenitalis. Folia Microbiol (Praha) 2013 Sep;58(5):375-84.
- Brooks BW, Lutze-Wallace CL, Maclean LL, Vinogradov E, Perry MB. Identification and differentiation of Taylorella equigenitalis and Taylorella asinigenitalis by lipopolysaccharide O-antigen serology using monoclonal antibodies. Can J Vet Res 2010 Jan;74(1):18-24.
- Tazumi A, Sekizuka T, Moore JE, Millar BC, Taneike I, Matsuda M. Molecular characterization of intervening sequences in 23S rRNA genes and 23S rRNA fragmentation in Taylorella equigenitalis. Folia Microbiol (Praha) 2008;53(6):486-92.
- Hansson I, Persson M, Svensson L, Engvall EO, Johansson KE. Identification of nine sequence types of the 16S rRNA genes of Campylobacter jejuni subsp. jejuni isolated from broilers. Acta Vet Scand 2008 May 21;50(1):10.
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