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Home / Equine Research Bank / J Clin Microbiol / Isolation of a mucoid alginate-producing Pseudomonas aerugin...
Journal of clinical microbiology1992; 30(3); 595-599; doi: 10.1128/jcm.30.3.595-599.1992

Isolation of a mucoid alginate-producing Pseudomonas aeruginosa strain from the equine guttural pouch.

Abstract: The isolation and characterization of a mucoid, alginate-producing strain of Pseudomonas aeruginosa from a nonhuman host, namely, in chondroids from an equine guttural pouch, is reported for the first time. Pure cultures of P. aeruginosa 12534 were isolated from a 17-month-old pony mare with a history of chronic bilateral mucopurulent nasal discharge from the right guttural pouch. Transmission electron microscopy of chondroids showed mucoid P. aeruginosa growing as microcolonies within a matrix of extracellular material. On the basis of expression of the mucoid phenotype under different growth conditions, P. aeruginosa 12534 belongs to group 1 and resembles other isolates carrying the muc-23 mutation. The bulk of the extracellular material was characterized as being alginate by chemical and 1H nuclear magnetic resonance analyses, which showed that it had a composition similar to that produced by isolates of P. aeruginosa from human patients with cystic fibrosis.
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Publication Date: 1992-03-01 PubMed ID: 1551975PubMed Central: PMC265116DOI: 10.1128/jcm.30.3.595-599.1992Google Scholar: Lookup
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Summary

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The research article outlines how scientists isolated and characterized an alginate-producing strain of Pseudomonas aeruginosa from the equine guttural pouch, marking the first time this bacterium has been identified in a non-human host.

Identification of Pseudomonas aeruginosa In Non-Human Host

  • The study represents the first reported case of isolating and characterizing a strain of Pseudomonas aeruginosa, specifically one that produces alginate, from a non-human host.
  • The host in question was equine, or horse. The bacterium was identified in chondroids from the horse’s guttural pouch, an air-filled sac found near the animal’s throat.
  • P. aeruginosa was isolated from a 17-month old female pony that had a chronic mucopurulent nasal discharge from the right guttural pouch.

Analysis of Bacterial Strain

  • Through transmission electron microscopy, the research team identified the newly isolated strain of P. aeruginosa growing as microcolonies in a matrix of extracellular material.
  • Based on the phenotypic expression under a variety of growth conditions, the isolated P. aeruginosa strain termed P. aeruginosa 12534, was categorized under group 1. This suggested that it closely resembled other isolates carrying the muc-23 mutation.

Nature of Extracellular Material

  • The principal component of the extracellular material was characterized as alginate, identified through chemical analysis and 1H nuclear magnetic resonance analysis.
  • Interestingly, the composition of the alginate was found to be similar to that produced by strains of P. aeruginosa isolated from human patients suffering from cystic fibrosis. This similarity may have implications for understanding the pathophysiology of P. aeruginosa infections and developing potential approaches to treatment.

Cite This Article

APA
Govan JR, Sarasola P, Taylor DJ, Tatnell PJ, Russell NJ, Gacesa P. (1992). Isolation of a mucoid alginate-producing Pseudomonas aeruginosa strain from the equine guttural pouch. J Clin Microbiol, 30(3), 595-599. https://doi.org/10.1128/jcm.30.3.595-599.1992

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 30
Issue: 3
Pages: 595-599

Researcher Affiliations

Govan, J R
  • Department of Medical Microbiology, University of Edinburgh Medical School, Scotland, United Kingdom.
Sarasola, P
    Taylor, D J
      Tatnell, P J
        Russell, N J
          Gacesa, P

            MeSH Terms

            • Alginates / metabolism
            • Animals
            • Eustachian Tube / microbiology
            • Female
            • Glucuronic Acid
            • Hexuronic Acids
            • Horse Diseases / microbiology
            • Horses / microbiology
            • Microscopy, Electron
            • Pseudomonas Infections / microbiology
            • Pseudomonas Infections / veterinary
            • Pseudomonas aeruginosa / growth & development
            • Pseudomonas aeruginosa / isolation & purification
            • Pseudomonas aeruginosa / metabolism

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            Citations

            This article has been cited 3 times.
            1. Pottier M, Castagnet S, Gravey F, Leduc G, Sévin C, Petry S, Giard JC, Le Hello S, Léon A. Antimicrobial Resistance and Genetic Diversity of Pseudomonas aeruginosa Strains Isolated from Equine and Other Veterinary Samples.. Pathogens 2022 Dec 30;12(1).
              doi: 10.3390/pathogens12010064pubmed: 36678412google scholar: lookup
            2. Pandey S, Delgado C, Kumari H, Florez L, Mathee K. Outer-membrane protein LptD (PA0595) plays a role in the regulation of alginate synthesis in Pseudomonas aeruginosa.. J Med Microbiol 2018 Aug;67(8):1139-1156.
              doi: 10.1099/jmm.0.000752pubmed: 29923820google scholar: lookup
            3. Muhammadi, Ahmed N. Genetics of bacterial alginate: alginate genes distribution, organization and biosynthesis in bacteria.. Curr Genomics 2007 May;8(3):191-202.
              doi: 10.2174/138920207780833810pubmed: 18645604google scholar: lookup
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