Isolation of equine herpesvirus-2 from the lung of an aborted fetus.
Abstract: This study describes the isolation of equine herpesvirus-2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti-EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclonal antibody against EHV-2. Restriction endonuclease DNA fingerprinting with BamHI also confirmed the identity of the virus as EHV-2. Furthermore, viral nucleic acid was detected by polymerase chain reaction from the original lung sample and from the DNA obtained from cells infected with the virus isolate. This work constitutes the first reported isolation of EHV-2 from an aborted equine fetus. The presence of EHV-2 in the lung of the aborted fetus would indicate that this virus is capable of crossing the placental barrier. However, no cause-effect relationship was established between the EHV-2 isolate and the abortion.
Publication Date: 2005-11-30 PubMed ID: 16312249DOI: 10.1177/104063870501700520Google Scholar: Lookup
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Summary
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The research article discusses isolating equine herpesvirus-2 (EHV-2) from the lung of a horse fetus that was aborted in Argentina. Further tests confirmed the presence of EHV-2 in the fetus, revealing that this virus can cross the placental barrier. However, the study did not establish a direct causal link between EHV-2 and the abortion.
Identification and Isolation of EHV-2
- The scientists isolated a virus from the lung tissue of a horse fetus that had been aborted. Upon examination, the virus was confirmed to be the Equine Herpesvirus-2 (EHV-2) strain.
- This confirmation was performed using various methods including indirect immunofluorescence – a test using a specialized rabbit anti-EHV-2 polyclonal antiserum – and a virus-neutralization test using an equine polyclonal antibody specific to EHV-2. The findings were additionally validated by DNAs unique fingerprint derived from a restriction endonuclease fingerprinting technique using BamHI.
Detection of Viral Nucleic Acid
- Besides the identification and isolation of EHV-2, viral nucleic acid was also detected in the original lung sample, as well as in the DNA extracted from cells infected with the virus isolate.
- The team used a process called polymerase chain reaction (PCR) to achieve this. Polymerase chain reaction is an established laboratory method used for amplifying DNA sequences.
Implications of the Findings
- This study constitutes the first reported instance of EHV-2 isolated from an aborted equine fetus. The presence of this typically nonpathogenic virus in the lung of an aborted fetus could imply that EHV-2 is capable of crossing the placental barrier – which normally protects the fetus from most infections.
- However, the authors of the research article noted that they couldn’t establish a cause-effect relationship between the EHV-2 isolate and the abortion. Thus, while the virus was present in the fetus, it cannot be definitively stated that EHV-2 was the direct cause of the abortion.
Cite This Article
APA
Galosi CM, de la Paz VC, Fernández LC, Martinez JP, Craig MI, Barrandeguy M, Etcheverrrigaray ME.
(2005).
Isolation of equine herpesvirus-2 from the lung of an aborted fetus.
J Vet Diagn Invest, 17(5), 500-502.
https://doi.org/10.1177/104063870501700520 Publication
Researcher Affiliations
- Department of Virology, Faculty of Veterinary Sciences, National University of La Plata, Buenos Aires Province, Argentina.
MeSH Terms
- Aborted Fetus / virology
- Animals
- DNA Fingerprinting / veterinary
- DNA, Viral / chemistry
- DNA, Viral / genetics
- Deoxyribonuclease BamHI / metabolism
- Female
- Fluorescent Antibody Technique, Indirect / veterinary
- Herpesviridae Infections / veterinary
- Herpesviridae Infections / virology
- Horse Diseases / virology
- Horses
- Lung / embryology
- Lung / virology
- Neutralization Tests / veterinary
- Polymerase Chain Reaction / veterinary
- Pregnancy
- Rhadinovirus / genetics
- Rhadinovirus / isolation & purification
- Tumor Virus Infections / veterinary
- Tumor Virus Infections / virology
Citations
This article has been cited 8 times.- de Almeida Campos AC, Cicolo S, de Oliveira CM, Molina CV, Navas-Suárez PE, Poltronieri Dos Santos T, da Silveira VB, Barbosa CM, Baccarin RYA, Durigon EL, Góes LGB, Belli CB, Oliveira DBL. Potential outbreak by herpesvirus in equines: detection, clinical, and genetic analysis of equid gammaherpesvirus 2 (EHV-2).. Braz J Microbiol 2023 Jun;54(2):1137-1143.
- Tong P, Duan R, Palidan N, Deng H, Duan L, Ren M, Song X, Jia C, Tian S, Yang E, Kuang L, Xie J. Outbreak of neuropathogenic equid herpesvirus 1 causing abortions in Yili horses of Zhaosu, North Xinjiang, China.. BMC Vet Res 2022 Mar 1;18(1):83.
- Tsai MS, Newman C, Macdonald DW, Buesching CD. Stress-Related Herpesvirus Reactivation in Badgers Can Result in Clostridium Proliferation.. Ecohealth 2021 Dec;18(4):440-450.
- Thorsteinsdóttir L, Jónsdóttir S, Stefánsdóttir SB, Andrésdóttir V, Wagner B, Marti E, Torsteinsdóttir S, Svansson V. The effect of maternal immunity on the equine gammaherpesvirus type 2 and 5 viral load and antibody response.. PLoS One 2019;14(6):e0218576.
- Dall Agnol AM, Beuttemmuller EA, Pilz D, Leme RA, Saporiti V, Headley SA, Alfieri AF, Alfieri AA. Detection of Equid gammaherpesvirus 2 and 5 DNA in the upper respiratory tract of asymptomatic horses from Southern Brazil.. Braz J Microbiol 2019 Jul;50(3):875-878.
- Muscat KE, Padalino B, Hartley CA, Ficorilli N, Celi P, Knight P, Raidal S, Gilkerson JR, Muscatello G. Equine Transport and Changes in Equid Herpesvirus' Status.. Front Vet Sci 2018;5:224.
- Marenzoni ML, Stefanetti V, Danzetta ML, Timoney PJ. Gammaherpesvirus infections in equids: a review.. Vet Med (Auckl) 2015;6:91-101.
- Marenzoni ML, Bietta A, Lepri E, Casagrande Proietti P, Cordioli P, Canelli E, Stefanetti V, Coletti M, Timoney PJ, Passamonti F. Role of equine herpesviruses as co-infecting agents in cases of abortion, placental disease and neonatal foal mortality.. Vet Res Commun 2013 Dec;37(4):311-7.
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