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Home / Equine Research Bank / Can J Comp Med / Isolation of herpesvirus from equine leukocytes: comparison ...
Canadian journal of comparative medicine : Revue canadienne de medecine comparee1970; 34(1); 59-65;

Isolation of herpesvirus from equine leukocytes: comparison with equine rhinopneumonitis virus.

Abstract: An agent which possessed the properties of herpesviruses was isolated from the leukocytes of 71 out of 80 (88.7%) apparently normal Iowa horses. It was ether- and heat-sensitive, DNA type, and produced type-A intranuclear inclusion bodies in cell cultures. Electron micrographs revealed a virion of typical herpesvirus structure. Leukocyte isolate virus could be differentiated from equine rhinopneumonitis virus (ERV) by serum neutralization, by growth differences in rabbit kidney cells, and by fluorescent antibody staining. Specific neutralizing antibody against this agent was found in a pooled serum sample from normal horses and in the serums of herpesvirus carrier horses. Serum from a mare inhibited growth of both ERV and leukocyte viral isolates. Normal sheep, calf, and rabbit serum did not neutralize either virus.
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Publication Date: 1970-01-01 PubMed ID: 4246005PubMed Central: PMC1319423
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates a type of herpesvirus, which was successfully isolated from horse white blood cells. The identified herpesvirus showed distinct characteristics that differentiate it from equine rhinopneumonitis virus.

Isolation of Herpesvirus from Equine Leukocytes

  • The researchers successfully isolated a herpesvirus from horse white blood cells (leukocytes). This virus was found in a total of 71 out of 80 horses (88.7%) that appeared to be healthy and belonged to the Iowa region.
  • This isolated herpesvirus had some unique properties – it was sensitive to ether and heat, was DNA type, and it produced type-A intranuclear inclusion bodies in cell cultures. The term “DNA type” means that its genetic material was DNA, not RNA. “Type-A intranuclear inclusion bodies” refers to small structures within a cell’s nucleus that can be indicative of certain viruses.
  • Upon examining through an electron microscope, it was confirmed that the virus had the typical structure of a herpesvirus.

Differentiation from Equine Rhinopneumonitis Virus

  • The researchers also differentiated the newly isolated herpesvirus from the equine rhinopneumonitis virus (ERV) by means of several tests. They observed different growth characteristics in rabbit kidney cells and immune responses (serum neutralization) to the two viruses. Furthermore, they used fluorescent antibody staining, a common method of identifying viruses under a microscope, to differentiate between them.
  • In terms of immunology, the researchers noticed the presence of specific neutralizing antibodies against the isolated herpesvirus in horse serum samples. These antibodies (proteins produced by the immune system to neutralize harmful pathogens) were found in both normal horses and horses that were carriers of this specific herpesvirus.
  • Interestingly, horse serum from a mare could suppress the growth of both viruses—ERV and the newly isolated herpesvirus. However, serum from other animals (normal sheep, calf, and rabbit) proved ineffective in neutralizing either virus.

Cite This Article

APA
Kemeny L, Pearson JE. (1970). Isolation of herpesvirus from equine leukocytes: comparison with equine rhinopneumonitis virus. Can J Comp Med, 34(1), 59-65.

Publication

Canadian journal of comparative medicine : Revue canadienne de medecine comparee
ISSN: 0008-4050
NlmUniqueID: 0151747
Country: Canada
Language: English
Volume: 34
Issue: 1
Pages: 59-65

Researcher Affiliations

Kemeny, L
    Pearson, J E

      MeSH Terms

      • Animals
      • Antibodies / isolation & purification
      • Cattle
      • Cell Line
      • Ethyl Ethers / pharmacology
      • Fluorescent Antibody Technique
      • Herpesviridae / drug effects
      • Herpesviridae / growth & development
      • Herpesviridae / isolation & purification
      • Horses / immunology
      • Immune Sera / pharmacology
      • Kidney
      • Leukocytes / microbiology
      • Microscopy, Electron
      • Neutralization Tests
      • Protein Denaturation
      • Rabbits
      • Sheep

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