Isolation of RNA from equine peripheral blood cells: comparison of methods.
Abstract: Gene expression studies in equine research involve the use of whole blood samples as a vital source of RNA. To determine the optimal method for RNA isolation from equine whole blood, we compared three RNA isolation strategies using different commercially available kits to evaluate the yield and quality of equine RNA. All 3 methods produced RNA with high quality. Though it did not produce the highest yield, combining the quality, yield and the need for the downstream application in our project, LeukoLOCK™ total RNA isolation system was the best RNA extraction method.
Publication Date: 2013-09-22 PubMed ID: 24133642PubMed Central: PMC3797321DOI: 10.1186/2193-1801-2-478Google Scholar: Lookup
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- Journal Article
Summary
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This study set out to identify the best method for extracting high-quality RNA from equine whole blood and determined the LeukoLOCK™ total RNA isolation system to be the most effective option.
Objective of the Study
- The research primarily aimed at ascertaining the optimal procedure for isolating RNA from the whole blood samples of horses.
- The main goal was to find a method that would result in high-quality RNA suitable for gene expression studies in equine research.
Research Methods
- The researchers compared three different methods of RNA isolation. These techniques were all based on commercially available RNA extraction kits.
- The criteria for comparison were the quantity of RNA produced (yield) and the quality of the isolated RNA.
Results of the Study
- All three extraction methods produced high-quality RNA, proving their effectiveness.
- In terms of yield, one method did not produce the highest amount of RNA, but this factor wasn’t the sole determinant of efficacy.
Conclusions of the Study
- While evaluating the yield, quality, and the requirement for downstream application in the project, the LeukoLOCK™ total RNA isolation system emerged as the best RNA extraction method.
- This system was therefore identified as the optimal method for isolating RNA from equine whole blood for gene expression studies in equine research.
Cite This Article
APA
Jiang Z, Uboh CE, Chen J, Soma LR.
(2013).
Isolation of RNA from equine peripheral blood cells: comparison of methods.
Springerplus, 2(1), 478.
https://doi.org/10.1186/2193-1801-2-478 Publication
Researcher Affiliations
- Department of Clinical Studies, University of Pennsylvania School of Veterinary Medicine, New Bolton Center Campus, 382 West Street Road, Kennett Square, Kragujevac, PA 19348 USA.
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Citations
This article has been cited 10 times.- Wang C, Liu H. Factors influencing degradation kinetics of mRNAs and half-lives of microRNAs, circRNAs, lncRNAs in blood in vitro using quantitative PCR. Sci Rep 2022 May 4;12(1):7259.
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