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Home / Equine Research Bank / PLoS Negl Trop Dis / Isolation of saint louis encephalitis virus from a horse wit...
PLoS neglected tropical diseases2013; 7(11); e2537; doi: 10.1371/journal.pntd.0002537

Isolation of saint louis encephalitis virus from a horse with neurological disease in Brazil.

Abstract: St. Louis encephalitis virus (SLEV) is a causative agent of encephalitis in humans in the Western hemisphere. SLEV is a positive-sense RNA virus that belongs to the Flavivirus genus, which includes West Nile encephalitis virus, Japanese encephalitis virus, Dengue virus and other medically important viruses. Recently, we isolated a SLEV strain from the brain of a horse with neurological signs in the countryside of Minas Gerais, Brazil. The SLEV isolation was confirmed by reverse-transcription RT-PCR and sequencing of the E protein gene. Virus identity was also confirmed by indirect immunofluorescence using commercial antibodies against SLEV. To characterize this newly isolated strain in vivo, serial passages in newborn mice were performed and led to hemorrhagic manifestations associated with recruitment of inflammatory cells into the central nervous system of newborns. In summary this is the first isolation of SLEV from a horse with neurological signs in Brazil.
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Publication Date: 2013-11-21 PubMed ID: 24278489PubMed Central: PMC3836713DOI: 10.1371/journal.pntd.0002537Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research documents the first occasion of the St. Louis encephalitis virus (SLEV) being isolated from a horse with neurological symptoms in Minas Gerais, Brazil.

Research Objectives

  • The primary objective of the research was to identify the cause behind neurological signs exhibited by a horse in Minas Gerais, Brazil.
  • A secondary aim was to characterize the isolated virus strain to understand its behaviour.

Method and Findings

  • The researchers successfully isolated a strain of the St. Louis encephalitis virus (SLEV) from the brain of the affected horse. The SLEV is a positive-sense RNA virus and is part of the Flavivirus genus, which includes other viruses responsible for serious diseases like West Nile encephalitis, Japanese encephalitis, and Dengue.
  • The isolation of SLEV was validated by conducting reverse-transcription RT-PCR and sequencing the E protein gene. Furthermore, the virus’s identity was confirmed by utilizing indirect immunofluorescence and commercial antibodies against SLEV.

Virus Characterization

  • To better understand the isolated virus strain, researchers performed a series of tests on newborn mice.
  • The virus strain exhibited hemorrhagic manifestations in the experimented mice and led to the recruitment of inflammatory cells into their central nervous system.

Significance of the Research

  • This research emphasizes the zoonotic potential of SLEV, demonstrating that it can infect horses and cause neurological disease, not solely being a human pathogen.
  • The study was pioneering in documenting the first ever isolation of SLEV from a horse showing neurological signs in Brazil. This could prove instrumental in understanding and curbing the spread of this virus in the horse population and potentially to humans.

Cite This Article

APA
Rosa R, Costa EA, Marques RE, Oliveira TS, Furtini R, Bomfim MR, Teixeira MM, Paixão TA, Santos RL. (2013). Isolation of saint louis encephalitis virus from a horse with neurological disease in Brazil. PLoS Negl Trop Dis, 7(11), e2537. https://doi.org/10.1371/journal.pntd.0002537

Publication

PLoS neglected tropical diseases
ISSN: 1935-2735
NlmUniqueID: 101291488
Country: United States
Language: English
Volume: 7
Issue: 11
Pages: e2537
PII: e2537

Researcher Affiliations

Rosa, Roberta
  • Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Costa, Erica Azevedo
    Marques, Rafael Elias
      Oliveira, Taismara Simas
        Furtini, Ronaldo
          Bomfim, Maria Rosa Quaresma
            Teixeira, Mauro Martins
              Paixão, Tatiane Alves
                Santos, Renato Lima

                  MeSH Terms

                  • Animals
                  • Brain / virology
                  • Brazil
                  • Encephalitis Virus, St. Louis / genetics
                  • Encephalitis Virus, St. Louis / isolation & purification
                  • Encephalitis, St. Louis / veterinary
                  • Encephalitis, St. Louis / virology
                  • Fluorescent Antibody Technique, Indirect
                  • Horse Diseases / virology
                  • Horses
                  • Mice
                  • Molecular Sequence Data
                  • RNA, Viral / genetics
                  • Reverse Transcriptase Polymerase Chain Reaction
                  • Sequence Analysis, DNA

                  Conflict of Interest Statement

                  The authors have declared that no competing interests exist.

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