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Home / Equine Research Bank / J Parasit Dis / Isolation of Toxoplasma gondii from the masseter muscles of ...
Journal of parasitic diseases : official organ of the Indian Society for Parasitology2024; 49(1); 121-129; doi: 10.1007/s12639-024-01744-5

Isolation of Toxoplasma gondii from the masseter muscles of equines destined for human consumption in a slaughterhouse in southern Brazil.

Abstract: The aim of this study was to isolate from equids destined for slaughter in a Brazilian slaughterhouse. A total of 354 equids were analyzed, with blood samples collected from all the animals and samples of masseter muscle and brain tissue collected from 319 animals. A serological test was conducted to detect equids with specific antibodies for . Molecular detection of by nested PCR was performed on the tissue samples collected. Tissue samples were tested by murine bioassay in an attempt to isolate either the parasite or the parasite DNA. Real-time PCR was performed on the brain samples from 11 mice which seroconverted after inoculation, to quantify the parasitic DNA. Genotyping was performed in masseter tissues samples from positive horses or from tissues of mice inoculated with masseter. The seroprevalence of infection was 19.2%. Nested PCR showed that 5.3% of the equines and 28.8% of the mice from the bioassays were positive for . Nine masseter muscle samples were positive (either on nested-PCR or in bioassay). Genotyping by PCR-RFLP was attempeted on all 12 isolates and was successful in seven, revealing six recombinant and one atypical genotype. The detection of DNA in masseter muscle tissue of horses destined for consumption clearly indicates a risk to human health.
© Indian Society for Parasitology 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
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Publication Date: 2024-09-25 PubMed ID: 39975622PubMed Central: PMC11832872DOI: 10.1007/s12639-024-01744-5Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study aimed to identify the presence of the parasite Toxoplasma gondii in the muscles of horses intended for human consumption in a Brazilian slaughterhouse.

Research Methods and Procedures

  • To achieve their objective, the researchers conducted tests on 354 horses that were headed for a slaughterhouse. They collected blood from each of these animals, while also obtaining samples of masseter muscle and brain tissue from 319 of them.
  • With these body fluid and tissue samples, the team performed serological tests to identify horses with specific antibodies for Toxoplasma gondii. This allowed them to determine whether the horses had been exposed to the parasite.
  • Molecular detection of Toxoplasma gondii was done via nested PCR (polymerase chain reaction) on the tissue samples. This is a highly accurate and sensitive technique used for amplifying and detecting specific DNA sequences.
  • Tissue samples were also tested using a murine bioassay, a method where samples are injected into mice to observe for any disease symptoms or seroconversion (development of specific antibodies to the injected antigen).
  • The team performed real-time PCR on brain samples from 11 mice that experienced seroconversion. This was done to quantify the amount of the parasite’s genetic material present.
  • The last stage involved genotyping of the masseter tissues from positive horses or the tissues from inoculated mice. Genotyping was performed using PCR-RFLP (polymerase chain reaction – restriction fragment length polymorphism), a technique that differentiates organisms based on the patterns their DNA fragments produce.

Findings

  • The results showed that 19.2% of the equines were seropositive for Toxoplasma gondii infection, indicating that they had been exposed to the parasite.
  • Through nested PCR, it was found out that 5.3% of the horses and a whopping 28.8% of the mice from the bioassays were positive for Toxoplasma gondii.
  • Of all the tests done, nine masseter muscle samples showed positive results, either via nested-PCR or through the bioassay.
  • When genotyping was attempted on all these 12 samples, it was successful in seven cases. Of these, six samples were recombinant and one displayed an atypical genotype.

Final Conclusion

  • This study successfully identified the presence of Toxoplasma gondii in masseter muscle tissue of horses meant for consumption, thereby suggesting a health risk for humans consuming the infected meat. Thus, the study recommends better screening procedures in slaughterhouses to alleviate such risks.

Cite This Article

APA
de Oliveira UV, Varjão JL, de Jesus Deiró AG, Maciel BM, Silva FL, Pinheiro AM, Gondim LFP, Munhoz AD. (2024). Isolation of Toxoplasma gondii from the masseter muscles of equines destined for human consumption in a slaughterhouse in southern Brazil. J Parasit Dis, 49(1), 121-129. https://doi.org/10.1007/s12639-024-01744-5

Publication

Journal of parasitic diseases : official organ of the Indian Society for Parasitology
ISSN: 0971-7196
NlmUniqueID: 9713059
Country: India
Language: English
Volume: 49
Issue: 1
Pages: 121-129

Researcher Affiliations

de Oliveira, Uillians Volkart
  • Faculdade de Ciências Sociais aplicadas, Itamaraju, Bahia 45,836-000 Brazil.
Varjão, José Luís
  • Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, Ilhéus, Bahia Brazil.
de Jesus Deiró, Ana Graziela
  • Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, Ilhéus, Bahia Brazil.
Maciel, Bianca Mendes
  • Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, Bahia Brazil.
Silva, Fabiana Lessa
  • Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, Ilhéus, Bahia Brazil.
Pinheiro, Alexandre Moraes
  • Centro de Ciências Agrárias, Biológicas e Ambientais, Universidade Federal do Recôncavo Baiano, Cruz das Almas, Bahia Brazil.
Gondim, Luis Fernando Pita
  • Departamento de Anatomia, Patologia e Clínicas, Universidade Federal da Bahia, Salvador, Bahia Brazil.
Munhoz, Alexandre Dias
  • Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, Ilhéus, Bahia Brazil.

Conflict of Interest Statement

Conflict of interestThe authors have no relevant financial or non-financial interests to disclose.

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