Joint dependent concentrations of bone alkaline phosphatase in serum and synovial fluids of horses with osteochondral injury: an analytical and clinical validation.
Abstract: Validate use of a commercially available immunoassay for measurement of bone alkaline phosphatase (BAP) in equine serum and synovial fluid (SF), and investigate the effects of osteochondral (OC) injury in horses on BAP concentrations in serum and SF. Methods: SF was collected from 37 joints of 34 Thoroughbred (TB) racehorses undergoing arthroscopic surgery for the removal of OC fragments from either the carpal joints (n=18) or the metacarpo-/metatarsophalangeal (MP) joints (n=19). SF was also obtained from 52 joints of 16 normal TB horses, collected bilaterally from carpal joints of 10 horses (n=40), and MP joints of six horses (n=12). Blood was obtained from all 50 horses. A commercially available immunoassay was validated and subsequently used to determine equine serum and SF BAP concentrations. Correlations to radiographic and arthroscopic scores were assessed. Results: BAP concentrations were significantly lower in serum from horses with OC injury in their carpal or MP joints than in serum from normal horses. SF BAP concentrations in normal and OC injured carpal joints were significantly higher than MP joints. BAP concentrations were significantly higher in SF from OC injured carpal joints than normal. BAP concentrations were affected by joint sampled, with age having a significant interaction. Concentrations of BAP in the serum (22U/L) and a ratio of SF to serum > or = 0.5 were predictive of OC injury. Radiographic and arthroscopic scores significantly correlated with serum BAP concentrations, and SF:serum BAP correlated with arthroscopic scores. Conclusions: Determination of serum and SF BAP concentrations may be beneficial in the investigation of early joint injury. Joint and injury dependent differences in BAP concentrations allowed the estimation of predictive value for identifying OC injury.
Publication Date: 2007-12-26 PubMed ID: 18162418DOI: 10.1016/j.joca.2007.11.008Google Scholar: Lookup
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- Journal Article
- Validation Study
Summary
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The research validates the use of a certain immunoassay for measuring bone alkaline phosphatase in horse serum and synovial fluid, and explores its implications in horse osteochondral injuries. The investigation reveals significant differences in BAP concentrations, based on joint location and injury status, which can help in early injury detection.
Background and Purpose of the Research
- This research aims to validate a commercially available immunoassay for the measurement of bone alkaline phosphatase (BAP) in horse serum and synovial fluid (SF).
- It examines the effects of osteochondral (OC) injury in horses on BAP concentrations in the serum and SF.
- The overall goal is to investigate the implications of these BAP concentrations, potentially aiding in early detection of joint injuries in horses.
Methods
- SF was collected from both injured and normal joints of Thoroughbred racehorses.
- The study used arthroscopic surgery for the collection of OC fragments from the carpal joints or the metacarpo-/metatarsophalangeal (MP) joints.
- It also used normal SF obtained from carpal joints and MP joints of healthy horses.
- Blood samples were taken from all the horses in the study, injured or non-injured.
- The validated immunoassay was used to determine BAP concentrations in the obtained serum and SF samples. These concentrations were then compared with radiographic and arthroscopic scores.
Results
- BAP concentrations were significantly lower in serum from horses with OC injury in their carpal or MP joints than in serum from normal horses.
- Conversely, BAP concentrations were significantly higher in SF from OC injured carpal joints than that from normal horses.
- The study also found that the joint sampled and the age of the horse significantly interacted with the BAP concentrations.
- identified BAP concentration thresholds in serum, SF, and the SF to serum ratio that were predictive of OC injury.
Conclusions
- Determining BAP concentrations in serum and SF might help in early detection of joint injuries.
- The study successively identified the dependency of BAP concentration on joint location and injury status, illustrating its predictive value for OC injuries.
Cite This Article
APA
Trumble TN, Brown MP, Merritt KA, Billinghurst RC.
(2007).
Joint dependent concentrations of bone alkaline phosphatase in serum and synovial fluids of horses with osteochondral injury: an analytical and clinical validation.
Osteoarthritis Cartilage, 16(7), 779-786.
https://doi.org/10.1016/j.joca.2007.11.008 Publication
Researcher Affiliations
- Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610-1432, USA. tumb016@umn.edu
MeSH Terms
- Alkaline Phosphatase / analysis
- Alkaline Phosphatase / blood
- Animals
- Arthroscopy
- Biomarkers / analysis
- Biomarkers / blood
- Carpus, Animal / injuries
- Cartilage, Articular / injuries
- Clinical Enzyme Tests / methods
- Fractures, Bone / diagnosis
- Fractures, Bone / diagnostic imaging
- Fractures, Bone / veterinary
- Fractures, Cartilage / diagnosis
- Fractures, Cartilage / diagnostic imaging
- Fractures, Cartilage / veterinary
- Horse Diseases / diagnosis
- Horse Diseases / diagnostic imaging
- Horses
- Immunoenzyme Techniques
- Male
- Radiography
- Reproducibility of Results
- Synovial Fluid / chemistry
Citations
This article has been cited 9 times.- Soroko-Dubrovina M, Górniak W, Zielińska P, Górniak A, Čebulj-Kadunc N, Korczyński M. Evaluation of Shiitake Mushroom (Lentinula edodes) Supplementation on the Blood Parameters of Young Thoroughbred Racehorses.. Animals (Basel) 2022 Nov 19;12(22).
- Soroko M, Górniak W, Zielińska P, Górniak A, Śniegucka K, Nawrot K, Korczyński M. Effect of Lentinula edodes on Morphological and Biochemical Blood Parameters of Horses.. Animals (Basel) 2022 Apr 25;12(9).
- McCoy AM, Secor EJ, Roady PJ, Gray SM, Klein J, Gutierrez-Nibeyro SD. Plantar osteochondral fragments in young Standardbreds are associated with minimal joint inflammation at the time of surgical removal.. Equine Vet J 2023 Jan;55(1):33-41.
- Mobasheri A, Trumble TN, Byron CR. Editorial: One Step at a Time: Advances in Osteoarthritis.. Front Vet Sci 2021;8:727477.
- Bertoni L, Jacquet-Guibon S, Branly T, Desancé M, Legendre F, Melin M, Rivory P, Hartmann DJ, Schmutz A, Denoix JM, Demoor M, Audigié F, Galéra P. Evaluation of Allogeneic Bone-Marrow-Derived and Umbilical Cord Blood-Derived Mesenchymal Stem Cells to Prevent the Development of Osteoarthritis in An Equine Model.. Int J Mol Sci 2021 Mar 2;22(5).
- Bertoni L, Jacquet-Guibon S, Branly T, Legendre F, Desancé M, Mespoulhes C, Melin M, Hartmann DJ, Schmutz A, Denoix JM, Galéra P, Demoor M, Audigié F. An experimentally induced osteoarthritis model in horses performed on both metacarpophalangeal and metatarsophalangeal joints: Technical, clinical, imaging, biochemical, macroscopic and microscopic characterization.. PLoS One 2020;15(6):e0235251.
- Fradinho MJ, Mateus L, Bernardes N, Bessa RJB, Caldeira RM, Ferreira-Dias G. Growth patterns, metabolic indicators and osteoarticular status in the Lusitano horse: A longitudinal study.. PLoS One 2019;14(7):e0219900.
- Byron CR, Trahan RA. Comparison of the Effects of Interleukin-1 on Equine Articular Cartilage Explants and Cocultures of Osteochondral and Synovial Explants.. Front Vet Sci 2017;4:152.
- Boyce MK, Trumble TN, Carlson CS, Groschen DM, Merritt KA, Brown MP. Non-terminal animal model of post-traumatic osteoarthritis induced by acute joint injury.. Osteoarthritis Cartilage 2013 May;21(5):746-55.
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