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Home / Equine Research Bank / J Virol / Kinetics of viral deoxyribonucleic acid, protein, and infect...
Journal of virology1968; 2(8); 793-804; doi: 10.1128/JVI.2.8.793-804.1968

Kinetics of viral deoxyribonucleic acid, protein, and infectious particle production and alterations in host macromolecular syntheses in equine abortion (herpes) virus-infected cells.

Abstract: Infection of exponential-phase suspension cultures of mouse fibroblast cells (L-M) with equine abortion virus (EAV) resulted in inhibition of cell growth and marked alterations in host metabolic processes. The synthesis of deoxyribonucleic acid (DNA) and ribonucleic acid was inhibited within 4 hr after infection and was suppressed by more than 90% by the time of maximal virus replication (14 to 18 hr). The overall rate of protein synthesis, however, was similar in uninfected and virus-producing cells as determined by measurements of net protein and isotope incorporation. The time course of viral DNA and protein synthesis and assembly into mature virus was determined with the inhibitors 5-fluorodeoxyuridine (FUdR) and cycloheximide, respectively. Thus, viral DNA synthesis was essentially completed at 14 hr, and viral protein and infectious virus synthesis was completed at 18 hr. Although the number of plaque-forming units (PFU) produced by FUdR-treated cells (10(3) to 10(4) PFU/ml) was at least 3 logs less than that produced by untreated cells, the yield of physical particles (as determined by electron microscopy) was approximately the same at 30 hr after infection. Besides being relatively non-infective, the particles produced in FUdR-treated cells appeared morphologically incomplete as they contained little or no nucleoid material.
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Publication Date: 1968-08-01 PubMed ID: 4302745PubMed Central: PMC375693DOI: 10.1128/JVI.2.8.793-804.1968Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the impact of the Equine Abortion Virus (EAV) on the growth and metabolic processes of mouse fibroblast cells. The study reveals that EAV inhibits cell growth and significantly suppresses DNA and RNA synthesis, but it does not change the overall rate of protein synthesis.

Viral Infection and Impact on Cell Growth and Metabolism

  • The researchers infected mouse fibroblast cells in exponential growth phase with EAV and observed the outcomes.
  • As a result of infection, cell growth inhibited and considerable changes emerged in the host metabolic processes.
  • The synthesis of both DNA and RNA in cells halted within 4 hours after infection and was suppressed to over 90% when the virus replication peaked (14 to 18 hours).

Overall Rate of Protein Synthesis

  • Despite the inhibition of cell growth and the reduction in DNA and RNA synthesis, the overall protein synthesis rate remained similar in both uninfected and virus-producing cells.
  • The consistency in protein synthesis was determined by measuring net protein and isotope incorporation.

Viral DNA and Protein Synthesis Timeline

  • The study also investigated the progression of viral DNA and protein synthesis and their assembly into mature virus particles using inhibitors FUdR and cycloheximide, respectively.
  • Most of the viral DNA synthesis was completed by the 14-hour mark, and viral protein synthesis and formation of infectious viruses were completed by the 18-hour mark.

Comparison of Virus Production in FUdR-Treated and Untreated Cells

  • Cells treated with FUdR produced significantly fewer plaque-forming units (a measure of virus quantity), at least three logs less than untreated cells though the quantity of physical viral particles observed under electron microscopy was consistent.
  • However, the viral particles produced in FUdR-treated cells were relatively non-infective and appeared morphologically incomplete, as they contained minimal or no nucleoid material, which is crucial for viral replication.

Cite This Article

APA
O'Callaghan DJ, Hyde JM, Gentry GA, Randall CC. (1968). Kinetics of viral deoxyribonucleic acid, protein, and infectious particle production and alterations in host macromolecular syntheses in equine abortion (herpes) virus-infected cells. J Virol, 2(8), 793-804. https://doi.org/10.1128/JVI.2.8.793-804.1968

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 2
Issue: 8
Pages: 793-804

Researcher Affiliations

O'Callaghan, D J
    Hyde, J M
      Gentry, G A
        Randall, C C

          MeSH Terms

          • Abortion, Veterinary
          • Animals
          • Carbon Isotopes
          • Culture Techniques
          • Cycloheximide / pharmacology
          • Cytopathogenic Effect, Viral
          • DNA, Viral / biosynthesis
          • Female
          • Fibroblasts
          • Floxuridine / pharmacology
          • Growth
          • Herpesviridae
          • Herpesviridae Infections / metabolism
          • Horse Diseases
          • Horses
          • Kinetics
          • Leucine / metabolism
          • Microscopy, Electron
          • Pregnancy
          • Protein Biosynthesis
          • RNA / biosynthesis
          • Uridine / metabolism
          • Viral Proteins / biosynthesis
          • Virus Replication

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          This article has been cited 22 times.
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