Kumiss Supplementation Reduces Oxidative Stress and Activates Sirtuin Deacetylases by Regulating Antioxidant System.

The research investigates the potential of kumiss, a fermented horse milk beverage, in reducing oxidative stress and inducing sirtuin deacetylases via the regulation of antioxidant systems.

Objective and Methodology

• The experiment was conducted to examine the impacts of kumiss on sirtuin deacetylases amid oxidative stress triggered by 1,2-dimethyl hydrazine (DMH).
• For the research, forty male mice of BALB/C species were categorized into four groups namely: control, kumiss, DMH, and kumiss + DMH. The dosage for each group varied, with kumiss receiving 2 x 10 cfu/mL, DMH receiving 20 mg/kg, and the final group receiving a combination of the two.
• Following a 20-week regimen, the researchers examined SIRT2 and SIRT3 protein expressions in the liver, colon, and kidney tissues of the mice subjects through western blotting, immunolocalizations, and inhibitory anti-oxidant activity analysis.

Findings

• The results showed that the DMH group had a decrease in SIRT2 and SIRT3 expressions in the liver, colon, and kidney tissues, which was further stimulated by kumiss reinforcement.
• More so, SIRT3 (a mitochondrial protein) immunostaining in the cell nuclei of tissues increased in response to treatments with kumiss.
• The researchers found out that the oxidative stress that was induced by DMH led to an increase in plasma 8-OH-2-deoxyguanosine, tissue oxidative stress index, and total oxidant capacity levels.
• Fascinatingly, kumiss supplementation was discovered to reduce these elevated levels while raising tissue total antioxidant capacity and reduced glutathione levels.

Conclusion

• In their research, the scientists concluded that making clear the molecular relationship between intracellular changes in the locations of SIRT2 and SIRT3 and oxidative stress could become crucial in enabling the development of new medical treatments in the future.
• In addition, they also concluded from their findings that kumiss might have a protective effect against DMH-induced damage by regulating the expression of sirtuin proteins and by safeguarding the antioxidant system.