Laboratory evaluation of aqueous humor in the healthy dog, cat, horse, and cow.
Abstract: Using routinely available clinical laboratory methods, aqueous humor samples were evaluated from 12 healthy dogs, 15 healthy cats, 7 healthy horses, and 6 healthy cows. Aqueous humor was almost acellular; cells that were present had degenerated beyond recognition. Protein concentration was low; only albumin was detectable on electrophoresis. Creatine phosphokinase and lactate dehydrogenase isoenzymes were not detected. Artifacts induced by sampling were insignificant compared with alterations in aqueous humor composition that occur with ocular diseases.
Publication Date: 1985-03-01 PubMed ID: 3994129
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- Journal Article
Summary
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The research article focuses on examining the characteristics of aqueous humor from various healthy animals such as dogs, cats, horses, and cows using routine clinical laboratory methods.
Sample Collection
- The researchers collected aqueous humor samples from a range of healthy animals, notably 12 dogs, 15 cats, 7 horses, and 6 cows.
Aqueous Humor Characteristics
- The study found that the aqueous humor was almost devoid of cells. More so, the scarce cells found were so degenerated that they could not be recognized.
- The protein concentration within the aqueous humor samples was predominantly low, with albumin being the only detectable protein on electrophoresis. Creatine phosphokinase and lactate dehydrogenase isoenzymes could not be detected.
Implications from Sampling
- The research pointed out that the artifacts (changes or events) brought about by the sampling process are insignificant when compared to alterations in the composition of aqueous humor that occur due to ocular diseases.
Significance of the Study
- The findings suggest that the composition of the aqueous humor in healthy animals varies little, regardless of the species. Therefore, any significant alterations in the composition of this fluid could be indicative of ocular diseases. This makes the examination of aqueous humor a potential diagnostic tool for eye diseases in animals.
Cite This Article
APA
Hazel SJ, Thrall MA, Severin GA, Lauerman LH, Lavach JD.
(1985).
Laboratory evaluation of aqueous humor in the healthy dog, cat, horse, and cow.
Am J Vet Res, 46(3), 657-659.
Publication
Researcher Affiliations
MeSH Terms
- Albumins / analysis
- Animals
- Aqueous Humor / cytology
- Cats / anatomy & histology
- Cattle / anatomy & histology
- Cell Count / veterinary
- Creatine Kinase / analysis
- Dogs / anatomy & histology
- Electrophoresis
- Eye Proteins / analysis
- Female
- Horses / anatomy & histology
- Isoenzymes
- L-Lactate Dehydrogenase / analysis
- Male
- Species Specificity
Citations
This article has been cited 4 times.- Zhou R, Horai R, Mattapallil MJ, Caspi RR. A new look at immune privilege of the eye: dual role for the vision-related molecule retinoic acid. J Immunol 2011 Oct 15;187(8):4170-7.
- Fischer U, Ertle R, Abel P, Rebrin K, Brunstein E, Hahn von Dorsche H, Freyse EJ. Assessment of subcutaneous glucose concentration: validation of the wick technique as a reference for implanted electrochemical sensors in normal and diabetic dogs. Diabetologia 1987 Dec;30(12):940-5.
- Hanna PE, Bellamy JE, Donald A. Postmortem eyefluid analysis in dogs, cats and cattle as an estimate of antemortem serum chemistry profiles. Can J Vet Res 1990 Oct;54(4):487-94.
- Stranieri A, Lauzi S, Paltrinieri S. Clinicopathological and Molecular Analysis of Aqueous Humor for the Diagnosis of Feline Infectious Peritonitis. Vet Sci 2024 May 9;11(5).
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